Rat IL-1 alpha/IL-1F1 Quantikine ELISA Kit

  (4 citations)     
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Product Details
Assay Procedure
Citations (4)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL)
  • Sensitivity
    4.12 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant rat IL-1 alpha
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Rat IL-1 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-1 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-1 alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat IL-1 alpha accurately. Results obtained using natural rat IL-1 alpha showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-1 alpha.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 54 134 417 54 130 449
Standard Deviation 3.6 6.3 16.5 5 9.5 29.7
CV% 6.7 4.7 4 9.3 7.3 6.6

Recovery

The recovery of rat IL-1 alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 104 100-111
EDTA Plasma (n=5) 98 89-112
Serum (n=4) 92 85-99
Linearity
To assess the linearity of the assay, samples spiked with rat IL-1 alpha in each matrix were diluted with Calibrator Diluent and then assayed.
 IL-1 alpha/IL-1F1 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1 alpha/IL-1F1
IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling.
  • Long Name:
    Interleukin 1 alpha
  • Entrez Gene IDs:
    3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
  • Alternate Names:
    Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Sample Type
  1. Age-, Gender-, andin VivoDifferent Doses of Isoproterenol Modifyin VitroAortic Vasoreactivity and Circulating VCAM-1
    Authors: B Nieto-Lima, A Cano-Martí, ME Rubio-Ruiz, I Pérez-Torr, V Guarner-La
    Front Physiol, 2018;9(0):20.
    Species: Mouse
    Sample Type: Plasma
  2. Intraarterial route increases the risk of cerebral lesions after mesenchymal cell administration in animal model of ischemia
    Authors: B Argibay, J Trekker, U Himmelreic, A Beiras, A Topete, P Taboada, M Pérez-Mato, A Vieites-Pr, R Iglesias-R, J Rivas, AM Planas, T Sobrino, J Castillo, F Campos
    Sci Rep, 2017;7(0):40758.
    Species: Rat
    Sample Type: Serum
  3. Membrane-Permeable Calpain Inhibitors Promote Rat Oral Mucosal Epithelial Cell Proliferation by Inhibiting IL-1alpha Signaling.
    Authors: Kondo M, Yamato M, Takagi R, Namiki H, Okano T
    PLoS ONE, 2015;10(7):e0134240.
    Species: Rat
    Sample Type: Cell Culture Supernates
  4. Regulation of interleukin 1alpha, activin and inhibin by lipopolysaccharide in Sertoli cells from prepubertal rats.
    Authors: Winnall WR, Okuma Y, Saito K
    Mol. Cell. Endocrinol., 2009;307(1):169-75.
    Species: Rat
    Sample Type: Cell Culture Supernates

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