Rat IL-3 Biotinylated Antibody

Catalog # Availability Size / Price Qty
IL‑3 in Rat Splenocytes.
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Product Details
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Rat IL-3 Biotinylated Antibody Summary

Species Reactivity
Detects rat IL‑3 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse IL-3 is observed and less than 1% cross-reactivity with recombinant human IL-3 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant rat IL‑3
Accession # NP_113701
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Rat IL‑3 (Catalog # 2524-RL)
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IL-3 antibody in Rat Splenocytes by Immunocytochemistry (ICC). View Larger

IL‑3 in Rat Splenocytes. IL-3 was detected in immersion fixed rat splenocytes using Goat Anti-Rat IL-3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2524) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-3

Rat interleukin-3 (IL-3; also multi-CSF) is a 26 kDa, variably glycosylated monomeric polypeptide that belongs to the alpha -helix family of hematopoietic cytokines (1, 2). IL-3 has pleiotrophic activies on a number of hematopoietic-related cells (1, 3). The rat molecule has two alternate splice forms. The first is termed IL-3 beta and is synthesized as a 169 amino acid (aa) precursor that contains a 27 aa signal sequence and a 142 aa mature segment (1, 2). The second is called IL-3 alpha, and is identical to IL-3 beta, save for a three amino acid (Tyr-Pro-Gln) deletion at positions 56-58 (1). The beta form is considered the most common form. Each form has an alpha -helical structure with two intrachain disulfide bonds and two potential N-linked glycosylation sites. Rat IL-3 is generally considered to be species-specific in its activity. In the mature region, rat IL-3 shares 55%, 30%, and 24% aa sequence identity with mouse, human, and bovine IL-3, respectively. Cells known to express IL-3 include connective tissue mast cells, astrocytes, microglia, megakaryocytes, eosinophils, T cells, keratinocytes and thymic epithelium.

IL-3 exerts its biological activities by binding to a 70 kDa, low affinity, ligand-binding IL-3 R alpha subunit, (6) which then recruits a 120 kDa, common beta -chain, signal transducing subunit (7) to form a functional IL-3 receptor (1, 6, 7). Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils, and various myeloid leukemic cells. IL-3 can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors including those for neutrophils, macrophages, magakaryocytes, and erythroid cells. IL-3 can stimulate the growth of early B cells and mature macrophages, mast cells, eosinophils, basophils, and megakaryocytes. IL-3 augments the function activity of basophils, mast cells, eosinophils, and macrophages (1, 8). In combination with other molecules such as CD40L and or IL-4, IL-3 can stimulate production of dendritic cells (1, 2, 9, 10).

  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Mangi, M.H. and A.C. Newland (1999) Cytokines Cell. Mol. Ther. 5:87.
  3. Esandi, M. del C. et al. (1998) Gene 211:151.
  4. Cohen, D.R. et al. (1986) Nucleic Acids Res. 14:3641.
  5. Gebicke-Haerter, P.J. et al. (1994) J. Neuroimmunol. 50:203.
  6. Chritton, S.C. and M.H. Sheng (1997) GenBank Accession # NP_640353.
  7. Appel, K. et al. (1995) J. Neurosci. 15:5800.
  8. Schrader, J.W. (2001) in Cytokine Reference, Oppenhiem, J.J. and M. Feldmann (eds): Academic Press, p. 855. 
  9. Ebner, S. et al. (2002) J. Immunol. 168:6199.
  10. Buelens C. et. al. (2002) Blood 99:993.
Long Name
Interleukin 3
Entrez Gene IDs
3562 (Human); 16187 (Mouse); 24495 (Rat)
Alternate Names
Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor

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