Rat Lipocalin-2/NGAL Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF3508
Product Details
Citations (1)
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Rat Lipocalin-2/NGAL Biotinylated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Lipocalin-2/NGAL in ELISAs and detects rat and mouse Lipocalin-2/NGAL in Western blots. In sandwich ELISAs, less than 0.4% cross-reactivity with recombinant mouse Lipocalin-2 is observed and less than 0.2% cross-reactivity with recombinant human (rh) Lipocalin-1 and rhLipocalin-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Lipocalin-2/NGAL
Gln21-Asn198
Accession # P30152
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat Lipocalin‑2/NGAL (Catalog # 3508-LC)

Rat Lipocalin-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Rat Lipocalin‑2/NGAL Antibody (Catalog # AF3508)

Standard: Recombinant Rat Lipocalin-2/NGAL Protein, CF (Catalog # 3508-LC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipocalin-2/NGAL

Lipocalin-2, also known as neutrophil gelatinase-associated Lipocalin and uterocalin (NGAL), has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (1‑3). It binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (K= 0.41 nM) (4). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9 nM) (4). Secretion of Lipocalin-2 in immune cells increases by stimulation of Toll-like receptor as a acute phase response to infection. As a result, it acts as a potent bacteriostatic reagents by sequestering iron (5). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (6). In the kidney, Lipocalin-2-mediated iron trafficking may be involved in protection from renal injury, and it has been implicated as a marker for early kidney failure (7, 8).

References
  1. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
  2. Devireddy, L.R. et al. (2001) Science 293:829.
  3. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
  4. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
  5. Flo, T.H. et al. (2004) Nature 432:917.
  6. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
  7. Mori, K. et al. (2005) J. Clin. Invest. 115:610.
  8. Mishra, J. et al. (2005) Lancet 365:1231.
Long Name
Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs
3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names
24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin

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Citation for Rat Lipocalin-2/NGAL Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Iron-related proteins: candidate urine biomarkers in childhood HIV-associated renal diseases.
    Authors: Soler-Garcia AA, Johnson D, Hathout Y, Ray PE
    Clin J Am Soc Nephrol, 2009;4(4):763-71.
    Species: Rat
    Sample Types: Urine
    Applications: ELISA Development

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