Rat Lipocalin-2/NGAL Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse and Rat Lipocalin‑2/NGAL by Western Blot.
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Product Details
Citations (6)
Supplemental Products

Rat Lipocalin-2/NGAL Antibody Summary

Species Reactivity
Detects rat Lipocalin-2/NGAL in ELISAs and detects rat and mouse Lipocalin-2/NGAL in Western blots. In sandwich ELISAs, less than 0.4% cross-reactivity with recombinant mouse (rm) Lipocalin-2/NGAL-2 is observed and less than 0.2% cross-reactivity with recombinant human (rh) Lipocalin-1/NGAL-1, rhLipocalin-2/NGAL-2 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant rat Lipocalin-2/NGAL
Accession # P30152
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Rat Lipocalin‑2/NGAL (Catalog # 3508-LC), see our available Western blot detection antibodies

Rat Lipocalin-2 Sandwich Immunoassay

Recommended Concentration
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Rat Lipocalin‑2/NGAL Biotinylated Antibody (Catalog # BAF3508)

Standard: Recombinant Rat Lipocalin-2/NGAL Protein, CF (Catalog # 3508-LC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat Lipocalin‑2/NGAL antibody by Western Blot. View Larger

Detection of Mouse and Rat Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of rat ovary tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3508) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lipocalin-2/NGAL at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipocalin-2/NGAL

Lipocalin-2, also known as neutrophil gelatinase-associated Lipocalin and uterocalin (NGAL), has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (1‑3). It binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (K= 0.41 nM) (4). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9 nM) (4). Secretion of Lipocalin-2 in immune cells increases by stimulation of Toll-like receptor as a acute phase response to infection. As a result, it acts as a potent bacteriostatic reagents by sequestering iron (5). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (6). In the kidney, Lipocalin-2-mediated iron trafficking may be involved in protection from renal injury, and it has been implicated as a marker for early kidney failure (7, 8).

  1. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
  2. Devireddy, L.R. et al. (2001) Science 293:829.
  3. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
  4. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
  5. Flo, T.H. et al. (2004) Nature 432:917.
  6. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
  7. Mori, K. et al. (2005) J. Clin. Invest. 115:610.
  8. Mishra, J. et al. (2005) Lancet 365:1231.
Long Name
Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs
3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names
24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin

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Citations for Rat Lipocalin-2/NGAL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Nephrotoxicity evaluation and proteomic analysis in kidneys of rats exposed to thioacetamide
    Authors: JY Lim, WW Jung, W Kim, KS Moon, D Sul
    Scientific Reports, 2022;12(1):6837.
    Species: Rat
    Sample Types: Tissue Lysates
    Applications: Western Blot
  2. Isolation of Hepatocytes from Liver Tissue by a Novel, Semi-Automated Perfusion Technology
    Authors: C Poggel, T Adams, R Janzen, A Hofmann, O Hardt, E Roeb, SK Schröder, CG Tag, M Roderfeld, R Weiskirche
    Biomedicines, 2022;10(9):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Depletion of Lipocalin 2 (LCN2) in Mice Leads to Dysbiosis and Persistent Colonization with Segmented Filamentous Bacteria
    Authors: P Klüber, SK Meurer, J Lambertz, R Schwarz, S Zechel-Gra, T Braunschwe, S Hurka, E Domann, R Weiskirche
    International Journal of Molecular Sciences, 2021;22(23):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Data on Lipocalin 2 and phosphatidylinositol 3-kinase signaling in a methionine- and choline-deficient model of non-alcoholic steatohepatitis
    Authors: A Asimakopou, E Borkham-Ka, EV de Leur, R Weiskirche
    Data Brief, 2017;13(0):644-649.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Iron-related proteins: candidate urine biomarkers in childhood HIV-associated renal diseases.
    Authors: Soler-Garcia AA, Johnson D, Hathout Y, Ray PE
    Clin J Am Soc Nephrol, 2009;4(4):763-71.
    Species: Rat
    Sample Types: Urine
    Applications: ELISA Development
  6. Expression and localisation of brain-type organic cation transporter (BOCT/24p3R/LCN2R) in the normal rat hippocampus and after kainate-induced excitotoxicity.
    Authors: Chia W, Tan F, Ong W, Dawe G
    Neurochem Int, 0;87(0):43-59.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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