Rat Lipocalin‑2/NGAL Antibody

Detection of Mouse Lipocalin-2/NGAL by Immunohistochemistry

Increased LCN2 expression in Esr1-deficient ovaries. Ovaries from wild-type (WT, n = 8) and Esr1 null mice (n = 6) were dissected and either embedded in paraffin or prepared for RNA analysis. (A) Hematoxylin and eosin (H & E) staining showed characteristic histological changes in Esr1-deficient compared to WT ovaries. (B) Immunohistochemical staining for LCN2 and ER alpha was performed. Sections were counterstained with hematoxylin (LCN2) or methyl green (ER alpha ). Magnifications: 100×, 400×; scale bars: 100 µm, 50 µm. (C) Tissue samples were subjected to immunofluorescence double staining for LCN2 and STAR. Magnifications: 100×; scale bars: 100 µm. (D) Lcn2 and Star expression was analyzed in ovarian tissues using RT-qPCR. Data are displayed as means ± SD. In case of normal distribution, Student’s t-tests were chosen for statistical analysis, otherwise a Mann–Whitney test was performed. Significant differences between groups are marked with asterisks: *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37298232), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Lipocalin-2/NGAL by Western Blot

LCN2 and ER alpha expression in the lung and kidney. Tissues from wild-type (WT, female: n = 9, male: n = 8) and Esr1 knockout (female: n = 6, male: n = 8) mice were either embedded in paraffin for immunostaining or processed for protein analysis. Immunohistochemistry staining was performed to localize LCN2 expression in (A) lung and (C) kidney tissue. Isotope-specific negative control IgG staining was performed to document antibody specificity. Magnifications: 100×; scale bar: 100 µm. LCN2 and ER alpha protein expression was analyzed in (B) lung and (D) kidney tissues by Western blot analysis in WT and Esr1 null mice. The expression of GAPDH (lungs) and vinculin (kidneys) was used to demonstrate equal protein loading. Unspecific bands are marked with asterisks (*) and are separated from specific bands by dashed lines. Protein expression of LCN2 was quantified densitometrically and plotted relative to the expression of the loading control. Mann–Whitney test (lungs) or Student’s t-tests (kidney) was used for statistical analysis. Significant differences between groups are indicated with asterisks: * p < 0.05. Data are displayed as means ± SD and non-significant results are marked with ns. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37298232), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Lipocalin-2/NGAL by Western Blot

LCN2 and ER alpha expression in the lung and kidney. Tissues from wild-type (WT, female: n = 9, male: n = 8) and Esr1 knockout (female: n = 6, male: n = 8) mice were either embedded in paraffin for immunostaining or processed for protein analysis. Immunohistochemistry staining was performed to localize LCN2 expression in (A) lung and (C) kidney tissue. Isotope-specific negative control IgG staining was performed to document antibody specificity. Magnifications: 100×; scale bar: 100 µm. LCN2 and ER alpha protein expression was analyzed in (B) lung and (D) kidney tissues by Western blot analysis in WT and Esr1 null mice. The expression of GAPDH (lungs) and vinculin (kidneys) was used to demonstrate equal protein loading. Unspecific bands are marked with asterisks (*) and are separated from specific bands by dashed lines. Protein expression of LCN2 was quantified densitometrically and plotted relative to the expression of the loading control. Mann–Whitney test (lungs) or Student’s t-tests (kidney) was used for statistical analysis. Significant differences between groups are indicated with asterisks: * p < 0.05. Data are displayed as means ± SD and non-significant results are marked with ns. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37298232), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Lipocalin-2/NGAL by Immunohistochemistry

Increased LCN2 expression in Esr1-deficient ovaries. Ovaries from wild-type (WT, n = 8) and Esr1 null mice (n = 6) were dissected and either embedded in paraffin or prepared for RNA analysis. (A) Hematoxylin and eosin (H & E) staining showed characteristic histological changes in Esr1-deficient compared to WT ovaries. (B) Immunohistochemical staining for LCN2 and ER alpha was performed. Sections were counterstained with hematoxylin (LCN2) or methyl green (ER alpha ). Magnifications: 100×, 400×; scale bars: 100 µm, 50 µm. (C) Tissue samples were subjected to immunofluorescence double staining for LCN2 and STAR. Magnifications: 100×; scale bars: 100 µm. (D) Lcn2 and Star expression was analyzed in ovarian tissues using RT-qPCR. Data are displayed as means ± SD. In case of normal distribution, Student’s t-tests were chosen for statistical analysis, otherwise a Mann–Whitney test was performed. Significant differences between groups are marked with asterisks: *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37298232), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Lipocalin-2/NGAL by Immunohistochemistry-Paraffin

Increased LCN2 expression in Esr1-deficient ovaries. Ovaries from wild-type (WT, n = 8) and Esr1 null mice (n = 6) were dissected and either embedded in paraffin or prepared for RNA analysis. (A) Hematoxylin and eosin (H & E) staining showed characteristic histological changes in Esr1-deficient compared to WT ovaries. (B) Immunohistochemical staining for LCN2 and ER alpha was performed. Sections were counterstained with hematoxylin (LCN2) or methyl green (ER alpha ). Magnifications: 100×, 400×; scale bars: 100 µm, 50 µm. (C) Tissue samples were subjected to immunofluorescence double staining for LCN2 and STAR. Magnifications: 100×; scale bars: 100 µm. (D) Lcn2 and Star expression was analyzed in ovarian tissues using RT-qPCR. Data are displayed as means ± SD. In case of normal distribution, Student’s t-tests were chosen for statistical analysis, otherwise a Mann–Whitney test was performed. Significant differences between groups are marked with asterisks: *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37298232), licensed under a CC-BY license. Not internally tested by R&D Systems.