Rat Lipocalin-2/NGAL Antibody AF3508: R&D Systems

Rat Lipocalin-2/NGAL Antibody

(2 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Detects rat Lipocalin-2/NGAL in ELISAs and detects rat and mouse Lipocalin-2/NGAL in Western blots. In sandwich ELISAs, less than 0.4% cross-reactivity with recombinant mouse (rm) Lipocalin-2/NGAL-2 is observed and less than 0.2% cross-reactivity with recombinant human (rh) Lipocalin-1/NGAL-1, rhLipocalin-2/NGAL-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat Lipocalin-2/NGAL
    Gln21-Asn198
    Accession # P30152
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Rat Lipocalin‑2/NGAL (Catalog # 3508-LC), see our available Western blot detection antibodies
    • Rat Lipocalin-2 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Rat Lipocalin‑2/NGAL Antibody (Catalog # AF3508)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Rat Lipocalin‑2/NGAL Biotinylated Antibody (Catalog # BAF3508)
  • ELISA Standard
     
    Recombinant Rat Lipocalin-2/NGAL Protein, CF (Catalog # 3508-LC)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse and Rat Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of rat ovary tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3508) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lipocalin‑2/NGAL at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lipocalin-2/NGAL

Lipocalin-2, also known as neutrophil gelatinase-associated Lipocalin and uterocalin (NGAL), has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (1‑3). It binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (K= 0.41 nM) (4). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9 nM) (4). Secretion of Lipocalin-2 in immune cells increases by stimulation of Toll-like receptor as a acute phase response to infection. As a result, it acts as a potent bacteriostatic reagents by sequestering iron (5). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (6). In the kidney, Lipocalin-2-mediated iron trafficking may be involved in protection from renal injury, and it has been implicated as a marker for early kidney failure (7, 8).

  • References:
    1. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
    2. Devireddy, L.R. et al. (2001) Science 293:829.
    3. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
    4. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
    5. Flo, T.H. et al. (2004) Nature 432:917.
    6. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
    7. Mori, K. et al. (2005) J. Clin. Invest. 115:610.
    8. Mishra, J. et al. (2005) Lancet 365:1231.
  • Long Name:
    Neutrophil Gelatinase-associated Lipocalin
  • Entrez Gene IDs:
    3934 (Human); 16819 (Mouse); 170496 (Rat)
  • Alternate Names:
    24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Data on Lipocalin 2 and phosphatidylinositol 3-kinase signaling in a methionine- and choline-deficient model of non-alcoholic steatohepatitis
    Authors: A Asimakopou, E Borkham-Ka, EV de Leur, R Weiskirche
    Data Brief, 2017;13(0):644-649.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Iron-related proteins: candidate urine biomarkers in childhood HIV-associated renal diseases.
    Authors: Soler-Garcia AA, Johnson D, Hathout Y, Ray PE
    Clin J Am Soc Nephrol, 2009;4(4):763-71.
    Species: Rat
    Sample Type: Urine
    Application: ELISA Development
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