Rat MIS RII Antibody

Catalog # Availability Size / Price Qty
AF1618
AF1618-SP
Product Details
Citations (4)
FAQs
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Rat MIS RII Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat MIS RII in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TGF‑ beta  RII, rhTGF-beta RIII, recombinant mouse (rm) TGF-beta RI, and rmTGF-beta RII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat MIS RII
Pro19-Pro144
Accession # Q62893
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat MIS RII Fc Chimera (Catalog # 1618-MR)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of rat ovary
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.05-0.2 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Rat MIS RII Fc Chimera (Catalog # 1618-MR) to immobilized Recombinant Human MIS/AMH (Catalog # 1737-MS) coated at 3 µg/mL (100 µL/well). At 2 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIS RII

Müllerian inhibiting substance (MIS), also named anti-Müllerian hormone (AMH) is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to fetal testis, plus postnatal testis and ovary (1). MIS induces Mullerian duct (female reproductive tract) regression during sexual differentiation in the male embryo and has been shown to have a regulatory role in gonads postnatally (1). Like other TGF-beta superfamily members, MIS signals via a heteromeric receptor complex consisting of a type I and a type II receptor serine/threonine kinase. Depending on the cell context, different type I receptors (including Act RIA/ALK2, BMP RIA/ALK3, and BMP RIB/ALK6) that are shared by other TGF-beta superfamily members, can be utilized for MIS signaling (1). In contrast, the type II MIS receptor (MIS RII) is unique and does not bind other TGF-beta superfamily members (1, 2). Upon ligand binding, MIS RII recruits the non-ligand binding type I receptor into the complex, resulting in phosphorylation the BMP-like signaling pathway effector proteins Smad1, Smad5 and Smad8 (1).

The gene for rat MIS RII was isolated separately by two groups working from Sertoli cell and fetal ovary cDNA libraries (3, 4). MIS RII comprises a 557 amino acid (aa) residue type I transmembrane protein with a putative 17 aa signal peptide. Mature MIS RII has a 127 aa cysteine-rich extracellular domain containing 2 potential N-glycosylation sites, a 21 aa transmembrane domain, and a 392 aa cytoplasmic region with a serine/threonine kinase domain (3, 4). Rat MIS RII shares 95% and 82% aa sequence identity with the mouse and human homologues, respectively (5). MIS RII is expressed in the mesenchymal cells surrounding the Mullerian ducts during embryonic development. Postnatally, it is expressed in uterine tissues and rodent Leydig cells, and coexpressed with MIS in the testicular Sertoli and ovarian granulosa cells (1, 6). The expression of MIS RII in the Mullerian mesenchyme is regulated by Wnt7a signaling from nearby epithelium through the canonical Wnt pathway. Wnt7a mutant mice do not express MIS RII, and do not experience Mullerian duct regression (7).

References
  1. Josso, N and N. diClemente (2003) Trends Endo. Met. 14:91.
  2. Mishna, Y. et al. (1999) Endocrinology 140:2084.
  3. Baarends, W. et al. (1994) Development 120:189.
  4. di Clement, N. et al. (1994) Mol. Endocrinol. 8:1006.
  5. Mishina, Y. et al. (1997) Biochem. Biophys. Res. Comm. 237:741.
  6. Teixeira, J. et al. (1996) Endocrinology 137:160.
  7. Hossain, A. and G. Saunders (2003) J. Biol. Chem. 278:26511.
Long Name
Mullerian-Inhibiting Substance Type II Receptor
Entrez Gene IDs
269 (Human); 110542 (Mouse); 29530 (Rat)
Alternate Names
AMH type II receptor; AMHR2; AMHREC 2.7.11.30; AMHRII; C14; MIS RII; MISR2; MISRII; MISRIIMIS type II receptor; MRII; Muellerian hormone type II receptor; Muellerian hormone type-2 receptor; Mullerian hormone receptor, type II; Mullerian inhibiting substance type II receptor

Product Datasheets

Citations for Rat MIS RII Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. A role for orphan nuclear receptor liver receptor homolog-1 (LRH-1, NR5A2) in primordial follicle activation
    Authors: MC Meinsohn, CHK Hughes, A Estienne, HD Saatcioglu, D Pépin, R Duggavathi, BD Murphy
    Scientific Reports, 2021;11(1):1079.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Defective AMH signaling disrupts GnRH neuron development and function and contributes to hypogonadotropic hypogonadism
    Authors: SA Malone, GE Papadakis, A Messina, NEH Mimouni, S Trova, M Imbernon, C Allet, I Cimino, J Acierno, D Cassatella, C Xu, R Quinton, G Szinnai, P Pigny, L Alonso-Cot, L Masgrau, JD Maréchal, V Prevot, N Pitteloud, P Giacobini
    Elife, 2019;8(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. Anti-M�llerian hormone: a new actor of sexual dimorphism in pituitary gonadotrope activity before puberty
    Authors: G Garrel, C Racine, D L'Hôte, C Denoyelle, CJ Guigon, N di Clement, J Cohen-Tann
    Sci Rep, 2016;6(0):23790.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Mullerian inhibiting substance contributes to sex-linked biases in the brain and behavior.
    Authors: Wang PY, Protheroe A, Clarkson AN, Imhoff F, Koishi K, McLennan IS
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(17):7203-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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