Rat MIS RII Antibody
Rat MIS RII Antibody Summary
Pro19-Pro144
Accession # Q62893
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MIS RII
Müllerian inhibiting substance (MIS), also named anti-Müllerian hormone (AMH) is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to fetal testis, plus postnatal testis and ovary (1). MIS induces Mullerian duct (female reproductive tract) regression during sexual differentiation in the male embryo and has been shown to have a regulatory role in gonads postnatally (1). Like other TGF-beta superfamily members, MIS signals via a heteromeric receptor complex consisting of a type I and a type II receptor serine/threonine kinase. Depending on the cell context, different type I receptors (including Act RIA/ALK2, BMP RIA/ALK3, and BMP RIB/ALK6) that are shared by other TGF-beta superfamily members, can be utilized for MIS signaling (1). In contrast, the type II MIS receptor (MIS RII) is unique and does not bind other TGF-beta superfamily members (1, 2). Upon ligand binding, MIS RII recruits the non-ligand binding type I receptor into the complex, resulting in phosphorylation the BMP-like signaling pathway effector proteins Smad1, Smad5 and Smad8 (1).
The gene for rat MIS RII was isolated separately by two groups working from Sertoli cell and fetal ovary cDNA libraries (3, 4). MIS RII comprises a 557 amino acid (aa) residue type I transmembrane protein with a putative 17 aa signal peptide. Mature MIS RII has a 127 aa cysteine-rich extracellular domain containing 2 potential N-glycosylation sites, a 21 aa transmembrane domain, and a 392 aa cytoplasmic region with a serine/threonine kinase domain (3, 4). Rat MIS RII shares 95% and 82% aa sequence identity with the mouse and human homologues, respectively (5). MIS RII is expressed in the mesenchymal cells surrounding the Mullerian ducts during embryonic development. Postnatally, it is expressed in uterine tissues and rodent Leydig cells, and coexpressed with MIS in the testicular Sertoli and ovarian granulosa cells (1, 6). The expression of MIS RII in the Mullerian mesenchyme is regulated by Wnt7a signaling from nearby epithelium through the canonical Wnt pathway. Wnt7a mutant mice do not express MIS RII, and do not experience Mullerian duct regression (7).
- Josso, N and N. diClemente (2003) Trends Endo. Met. 14:91.
- Mishna, Y. et al. (1999) Endocrinology 140:2084.
- Baarends, W. et al. (1994) Development 120:189.
- di Clement, N. et al. (1994) Mol. Endocrinol. 8:1006.
- Mishina, Y. et al. (1997) Biochem. Biophys. Res. Comm. 237:741.
- Teixeira, J. et al. (1996) Endocrinology 137:160.
- Hossain, A. and G. Saunders (2003) J. Biol. Chem. 278:26511.
Product Datasheets
Citations for Rat MIS RII Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
4
Citations: Showing 1 - 4
Filter your results:
Filter by:
-
A role for orphan nuclear receptor liver receptor homolog-1 (LRH-1, NR5A2) in primordial follicle activation
Authors: MC Meinsohn, CHK Hughes, A Estienne, HD Saatcioglu, D Pépin, R Duggavathi, BD Murphy
Scientific Reports, 2021;11(1):1079.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Defective AMH signaling disrupts GnRH neuron development and function and contributes to hypogonadotropic hypogonadism
Authors: SA Malone, GE Papadakis, A Messina, NEH Mimouni, S Trova, M Imbernon, C Allet, I Cimino, J Acierno, D Cassatella, C Xu, R Quinton, G Szinnai, P Pigny, L Alonso-Cot, L Masgrau, JD Maréchal, V Prevot, N Pitteloud, P Giacobini
Elife, 2019;8(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Anti-M�llerian hormone: a new actor of sexual dimorphism in pituitary gonadotrope activity before puberty
Authors: G Garrel, C Racine, D L'Hôte, C Denoyelle, CJ Guigon, N di Clement, J Cohen-Tann
Sci Rep, 2016;6(0):23790.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Mullerian inhibiting substance contributes to sex-linked biases in the brain and behavior.
Authors: Wang PY, Protheroe A, Clarkson AN, Imhoff F, Koishi K, McLennan IS
Proc. Natl. Acad. Sci. U.S.A., 2009;106(17):7203-8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Rat MIS RII Antibody
There are currently no reviews for this product. Be the first to review Rat MIS RII Antibody and earn rewards!
Have you used Rat MIS RII Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
