TGN38 (Trans-Golgi network integral membrane protein TGN38) is an 80-100 kDa integral membrane protein that is associated with intracellular trafficking. Its name derives from the fact that its predicted MW is 38 kDa. Although TGN38 cycles continuously between the Golgi and plasma membrane via endosomal vesicles, it is principally localized to the Golgi apparatus. Functionally, TGN38 is involved in both the formation of secretory vesicles, and in the process of clathrin-mediated endocytosis. This is accomplished through the creation of a 250 kDa cytoplasmic complex composed of a Tgoln1 dimer, Rab6, and other molecules that interact with the F-actin binding proteins neurabin-I and -II. This interaction likely contributes to directional vesicle trafficking. Mature rat Tgoln1 is a type I transmembrane glycoprotein that is 340 amino acids (aa) in length. It contains a 286 aa extracellular/luminal region (aa 18-303) plus a 33 aa cytoplasmic domain (aa 325-357). The luminal region appears to be heavily glycosylated with both O- and N-linked carbohydrate, some of which terminates in polysialylation; the cytoplasmic domain contains a cytosolic trafficking motif that encompases aa 348-353. There is one splice variant (TGN41) that contains a 26 aa substitution for the three C-terminal aa of TGN38. It has been suggested that this may heterodimerize with TGN38. Over aa 18-303, rat TGN38/Tgoln1 shares 67% aa sequence identity with the mouse ortholog to rat Tgoln1.
Rat TGN38 Alexa Fluor® 700‑conjugated Antibody
R&D Systems | Catalog # AF8059N
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Leu18-Ser303
Accession # P19814
Specificity
Clonality
Host
Isotype
Applications for Rat TGN38 Alexa Fluor® 700‑conjugated Antibody
Immunocytochemistry
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: TGN38
Long Name
Alternate Names
Gene Symbol
UniProt
Additional TGN38 Products
Product Documents for Rat TGN38 Alexa Fluor® 700‑conjugated Antibody
Certificate of Analysis
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Product Specific Notices for Rat TGN38 Alexa Fluor® 700‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars