Rat TGN38 Antibody

Catalog # Availability Size / Price Qty
AF8059
AF8059-SP
Detection of Rat TGN38 by Western Blot.
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Rat TGN38 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat TGN38 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse TGN38 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant rat TGN38
Leu18-Ser303
Accession # P19814
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat TGN38 antibody by Western Blot. View Larger

Detection of Rat TGN38 by Western Blot. Western blot shows lysates of RBL-2H3 rat basophilic leukemia cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Rat TGN38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8059) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TGN38 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry TGN38 antibody in RBL-2H3 Rat Cell Line by Immunocytochemistry (ICC). View Larger

TGN38 in RBL-2H3 Rat Cell Line. TGN38 was detected in immersion fixed RBL-2H3 rat basophilic leukemia cell line using Sheep Anti-Rat TGN38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8059) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi vesicles in cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGN38

TGN38 (Trans-Golgi network integral membrane protein TGN38) is an 80-100 kDa integral membrane protein that is associated with intracellular trafficking. Its name derives from the fact that its predicted MW is 38 kDa. Although TGN38 cycles continuously between the Golgi and plasma membrane via endosomal vesicles, it is principally localized to the Golgi apparatus. Functionally, TGN38 is involved in both the formation of secretory vesicles, and in the process of clathrin-mediated endocytosis. This is accomplished through the creation of a 250 kDa cytoplasmic complex composed of a Tgoln1 dimer, Rab6, and other molecules that interact with the F-actin binding proteins neurabin-I and -II. This interaction likely contributes to directional vesicle trafficking. Mature rat Tgoln1 is a type I transmembrane glycoprotein that is 340 amino acids (aa) in length. It contains a 286 aa extracellular/luminal region (aa 18-303) plus a 33 aa cytoplasmic domain (aa 325-357). The luminal region appears to be heavily glycosylated with both O- and N-linked carbohydrate, some of which terminates in polysialylation; the cytoplasmic domain contains a cytosolic trafficking motif that encompases aa 348-353. There is one splice variant (TGN41) that contains a 26 aa substitution for the three C-terminal aa of TGN38. It has been suggested that this may heterodimerize with TGN38. Over aa 18-303, rat TGN38/Tgoln1 shares 67% aa sequence identity with the mouse ortholog to rat Tgoln1.

Long Name
Trans-golgi Network Protein
Entrez Gene IDs
10618 (Human); 22134 (Mouse); 192152 (Rat)
Alternate Names
TGN38; TGN46; TGN46TGN38 homolog; TGN48MGC14722; TGN51; TGN5148, 51kD isoforms); Tgoln1; Tgoln2; trans-golgi network protein 2; Trans-Golgi network protein TGN51; Ttgn1

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Rat TGN38 Antibody

There are currently no reviews for this product. Be the first to review Rat TGN38 Antibody and earn rewards!

Have you used Rat TGN38 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review