Tissue inhibitors of metalloproteinases (TIMPs) are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family: TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 32‑34 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.
Key Product Details
Species Reactivity
Rat
Applications
Western Blot, ELISA Detection (Matched Antibody Pair)
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Loading...
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat TIMP-1
Cys24-Ala217
Accession # P30120
Cys24-Ala217
Accession # P30120
Specificity
Detects rat TIMP-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) TIMP-1, recombinant mouse (rm) TIMP-1, rhTIMP-2, rhTIMP-3, and rhTIMP-4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rat TIMP-1 Antibody [Biotin]
Rat TIMP-1 ELISA Standard Curve
Recombinant Rat TIMP‑1 (Catalog # 580-RT) was serially diluted and captured by Mouse Anti-Rat TIMP‑1 Monoclonal Antibody (Catalog # MAB5802) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF580) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Rat TIMP-1 Antibody [Biotin]
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Rat TIMP‑1 (Catalog # 580-RT)
Sample: Recombinant Rat TIMP‑1 (Catalog # 580-RT)
Rat TIMP-1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TIMP-1
Long Name
Tissue Inhibitors of Metalloproteinases 1
Alternate Names
TIMP1
Gene Symbol
TIMP1
UniProt
Additional TIMP-1 Products
Product Documents for Rat TIMP-1 Antibody [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Rat TIMP-1 Antibody [Biotin]
For research use only
Related Research Areas
Customer Reviews for Rat TIMP-1 Antibody [Biotin]
There are currently no reviews for this product. Be the first to review Rat TIMP-1 Antibody [Biotin] and earn rewards!
Have you used Rat TIMP-1 Antibody [Biotin]?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...