Rat UNC5H1 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1405
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Rat UNC5H1 Biotinylated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat UNC5H1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant rat UNC5H2 and recombinant human UNC5H4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat UNC5H1
Gln26-Asp358
Accession # O08721
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat UNC5H1 Fc Chimera (Catalog # 1405-UN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UNC5H1

Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues, UNC5H1-4, UNC5A-D, and rostral cerebellar malformation (RCM), are transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. All UNC5 family members have two Ig and two thrombospondin type 1 domains in their extracellular regions, as well as a conserved ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2). Rat UNC5H1 cDNA encodes a 898 amino acid (aa) residues type I membrane protein with a putative 25 aa signal peptide and 332 aa extracellular domain. The extracellular domain of rat UNC5H1 shares approximately 98% and 65% amino acid sequence identity with mouse UNC5H1and rat UNC5H2, respectively.

UNC5 family proteins are receptors for the netrin/UNC6 family of secreted axon guidance cues that are laminin-related proteins. Netrin family proteins can act as a chemoattractant for some axons and as a chemorepellent for others. Besides UNC5, netrin family proteins also bind to the DCC family of type I transmembrane receptors and to adenosine A2b receptor, a G protein-coupled seven-transmembrane receptor belonging to the adenosine receptor family (3, 4). In vitro, netrin binding to DCC family receptors in the absence of UNC5 is associated with axon attraction. However, the DCC-mediated attraction to netrin is converted to repulsion by binding of UNC5 to the DCC-netrin complex. In vivo, the mechanisms of netrin-dependent axon attraction and repulsion are more complex and may include UNC5‑mediated repulsion that is independent of DCC (1, 5). Besides their roles in axon guidance and neuronal migration, the UNC5 and DCC families also act as dependence receptors and exert pro-apoptotic effects in the absence of netrin (6).

References
  1. Hong, K. et al. (1999) Cell 97:927. 
  2. Leonardo, E.D. et al. (1997) Nature 386:833.
  3. Culotti, J.B. and D.C. Merz (1998) Curr. Opin. Cell Biol. 10:609.
  4. Corset, V. (2000) Nature 407:747.
  5. Merz, D.C. (2001) Genetics 158:1071.
  6. Llambi, F. et al. (2001) EMBO Journal 20:2715.
Long Name
Netrin Receptor UNC5A
Entrez Gene IDs
90249 (Human); 60629 (Rat)
Alternate Names
KIAA1976netrin receptor UNC5A; netrin receptor Unc5h1; Protein unc-5 homolog 1; Protein unc-5 homolog A; unc5 (C.elegans homolog) a; unc-5 homolog 1; unc-5 homolog A (C. elegans); UNC5A; UNC5H1; UNC5H1FLJ16449

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