Rat UNC5H1 Antibody

(1 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Detects rat UNC5H1 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant rat UNC5H2, recombinant human (rh) UNC5H3 and rhUNC5H4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat UNC5H1
    Gln26-Asp358
    Accession # O08721
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Rat UNC5H1 Fc Chimera (Catalog # 1405-UN)
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 2-6 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of Recombinant Chicken Netrin-1 (Catalog # 128-N1) to immobilized Recombinant Rat UNC5H1 Fc Chimera (Catalog # 1405-UN) coated at 2 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
UNC5H1 in Rat Cortical Stem Cells. UNC5H1 was detected in immersion fixed rat cortical stem cells differentiated by growth factor withdrawal for 7 days using Goat Anti-Rat UNC5H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1405) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: UNC5H1

Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues, UNC5H1-4, UNC5A-D, and rostral cerebellar malformation (RCM), are transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. All UNC5 family members have two Ig and two thrombospondin type 1 domains in their extracellular regions, as well as a conserved ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2). Rat UNC5H1 cDNA encodes a 898 amino acid (aa) residues type I membrane protein with a putative 25 aa signal peptide and 332 aa extracellular domain. The extracellular domain of rat UNC5H1 shares approximately 98% and 65% amino acid sequence identity with mouse UNC5H1and rat UNC5H2, respectively.

UNC5 family proteins are receptors for the netrin/UNC6 family of secreted axon guidance cues that are laminin-related proteins. Netrin family proteins can act as a chemoattractant for some axons and as a chemorepellent for others. Besides UNC5, netrin family proteins also bind to the DCC family of type I transmembrane receptors and to adenosine A2b receptor, a G protein-coupled seven-transmembrane receptor belonging to the adenosine receptor family (3, 4). In vitro, netrin binding to DCC family receptors in the absence of UNC5 is associated with axon attraction. However, the DCC-mediated attraction to netrin is converted to repulsion by binding of UNC5 to the DCC-netrin complex. In vivo, the mechanisms of netrin-dependent axon attraction and repulsion are more complex and may include UNC5‑mediated repulsion that is independent of DCC (1, 5). Besides their roles in axon guidance and neuronal migration, the UNC5 and DCC families also act as dependence receptors and exert pro-apoptotic effects in the absence of netrin (6).

  • References:
    1. Hong, K. et al. (1999) Cell 97:927. 
    2. Leonardo, E.D. et al. (1997) Nature 386:833.
    3. Culotti, J.B. and D.C. Merz (1998) Curr. Opin. Cell Biol. 10:609.
    4. Corset, V. (2000) Nature 407:747.
    5. Merz, D.C. (2001) Genetics 158:1071.
    6. Llambi, F. et al. (2001) EMBO Journal 20:2715.
  • Long Name:
    Netrin Receptor UNC5A
  • Entrez Gene IDs:
    90249 (Human); 60629 (Rat)
  • Alternate Names:
    KIAA1976netrin receptor UNC5A; netrin receptor Unc5h1; Protein unc-5 homolog 1; Protein unc-5 homolog A; unc5 (C.elegans homolog) a; unc-5 homolog 1; unc-5 homolog A (C. elegans); UNC5A; UNC5H1; UNC5H1FLJ16449
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
    Authors: Hoang S, Liauw J, Choi M
    J. Cereb. Blood Flow Metab., 2009;29(2):385-97.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
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