Rat/Xenopus Potassium Channel Kv2.2 Antibody

Voltage Gated
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Detection of Potassium Channel Kv2.2 by Western Blot
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Rat/Xenopus Potassium Channel Kv2.2 Antibody Summary

Species Reactivity
Rat, Xenopus
Rodent and Xenopus ~125 kDa Kv2.2 subunit of the Voltage Gated Potassium Channel protein.
Polyclonal Rabbit IgG
Antigen Affinity-purified
Peptide from the Kv2.2 subunit region of Xenopus and rodent, conjugated to keyhole limpet hemocyanin (KLH)
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.


Recommended Concentration
Western Blot
1:1000 dilution
See below
1:1000 dilution
Frozen sections; unpublished observations

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Potassium Channel Kv2.2 antibody by Western Blot View Larger

Detection of Potassium Channel Kv2.2 by Western Blot Western blot of rat brain homogenate. As shown in the autoradiograph, the antibody is specific for the ~125 kDa Voltage Gated Potassium Channel, Kv2.2 subunit. The labeling is blocked by preadsorption with the immunizing peptide.

Reconstitution Calculator

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: Potassium Channel Kv2.2

The rat potassium channel Kv2.2 subunit is a 95 - 125 kDa, six transmembrane glycoprotein that belongs to the B subfamily of the potassium channel family. It generates a voltage-dependent channel selective for potassium that helps determine the electrical property of smooth muscle cells, various epithelial cell types and neurons. The symbol K stands for potassium, v for the molecule’s ligand (i.e. voltage), 2 for the family type that is determined by similarity to fruit fly genes, and 2 for the gene number within the family. There are two genes within the Kv2 family (Kv2.1 and Kv2.2) that, although co-expressed, preferentially generate homotetramers rather than heterotetramers. Kv2 family members are associated with action potential repolarization. Kv2.2 is particularly noted for setting the resting membrane potential. Low Kv2.2 activity results in hypersensitive neurons.

  1. Trimmer, J.S. and K.J. Rhodes (2004) Annu. Rev. Physiol. 66:477.
  2. Malin, S.A. and J.M. Nerbonne (2002) J. Neurosci. 22:10094.
  3. Blaine, J.T. and A.B. Ribera (1998) J. Neurosci. 18:9585.
  4. Hwang, P.M. et al. (1992) Neuron 8:473.
  5. O’Grady, S.M. and S.Y. Lee (2005) Int. J. Biochem. Cell Biol. 37:1578.
Entrez Gene IDs
9312 (Human); 98741 (Mouse); 117105 (Rat)
Alternate Names
delayed rectifier potassium channel protein; KCNB2; KV2.2; Potassium Channel Kv2.2; potassium voltage-gated channel subfamily B member 2; potassium voltage-gated channel, Shab-related subfamily, member 2; voltage-gated potassium channel subunit Kv2.2

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