RbAp46 Antibody

Western Blot: RbAp46 Antibody [NBP3-32897]

Western Blot: RbAp46 Antibody [NBP3-32897] - Western blot analysis of RbAp46 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP3-32897, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: Hela cell lysate
Lane 3: NIH/3T3 cell lysate

Immunohistochemistry: RbAp46 Antibody [NBP3-32897]

Immunohistochemistry: RbAp46 Antibody [NBP3-32897] - Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-RbAp46 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32897, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.