RBFOX3/NeuN Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89821

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RBFOX3/NeuN Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PTQDYSGQTPVPTEHGMTLYTPAQTHPEQPGSEASTQPIAGTQTVPQTDEAAQTDSQPLHPSDPTEKQQ

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24747576)

Marker

Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RBFOX3/NeuN Antibody - BSA Free

RBFOX3/NeuN Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody - BSA Free [NBP1-89821]

Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody - BSA Free [NBP1-89821]

Staining of human cell line A549 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-89821]

Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-89821]

Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-89821] - Analysis in human cerebral cortex and tonsil tissues. Corresponding RBFOX3/NeuN RNA-seq data are presented for the same tissues.
RBFOX3/NeuN Antibody - BSA Free Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody - BSA Free [NBP1-89821]

Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody - BSA Free [NBP1-89821]

Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-89821]

Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-89821]

Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-89821] - Staining of human tonsil shows no positivity in germinal center cells as expected.
Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-89821]

Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-89821]

Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-89821] - Staining of human cerebellum shows strong nuclear positivity in cells in granular layer.
RBFOX3/NeuN Antibody - BSA Free Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody - BSA Free [NBP1-89821]

Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody - BSA Free [NBP1-89821]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-89821]

Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-89821]

Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-89821] - Staining of human hippocampus shows strong nuclear and cytoplasmic positivity in pyramidal cells.
RBFOX3/NeuN Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: RBFOX3/NeuN Antibody - BSA Free [NBP1-89821]

Chromatin Immunoprecipitation-exo-Seq: RBFOX3/NeuN Antibody - BSA Free [NBP1-89821]

ChIP-Exo-Seq composite graph for Anti-RBFOX3 (NBP1-89821) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for RBFOX3/NeuN Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBFOX3/NeuN

Rbfox3 (also known as Fox-3, Fox1 homolog C, Hrnbp3, and D11Bwg0517e) is one of three mammalian members of the RNA-binding protein Rbfox gene family, all of which are involved in regulating alternative RNA splicing. Originally identified as an epitope, NeuN is located within the N-terminal region (6-15 amino acids) of Rbfox3. Rbfox family members are highly conserved, having a single RNA recognition motif (RRM)-type RNA binding domain (RBD) near the center of the protein sequence. The RBD amino acid (aa) sequence is identical between members, Rbfox1 and Rbfox2, with 4 differences within the 77 aa domain of Rbfox3. The fox3 gene is comprised of 15 exons in humans, 11 exons in rat, with 3 variants in mouse containing 14 exons and another 3 variants containing 15 exons. The Rbfox3 protein is highly conserved across human, mouse, and rat with 98.9% sequence identity between mouse (isoform I) and rat and 83.9% sequence identity between mouse (isoform I) and human. The expression of Rbfox3/NeuN is restricted to the nervous system and has widely been used in stroke research. It is recognized as a marker of mature neuronal cell types in the spinal cord, cerebral cortex, hippocampus, dorsal thalamus, caudate/putamen, and cerebellum. NeuN has been shown to bind DNA and is predominantly nuclear. Differences in immunoreactivity have been reported between Rbfox3/NeuN subtypes in which the 46kDa is mainly found in the nucleus whereas the 48kDa form is primarily distributed in the cytoplasm (1,2).

Rbfox proteins participate in regulation of alternative splicing between family members as well as in autoregulation. While the breadth of brain and muscle specific targets for alternative splicing is well established for Rbfox proteins, those specific to Rbfox3/NeuN along with its alternative splicing mechanism are less understood. Rbfox3 has been shown to regulate neuronal differentiation by alternative splicing of Numb pre-mRNA and has a role in adult neurogenesis. Dysfunctional Rbfox3/NeuN has been associated with various neurological disorders such as neurodevelopmental delay, autism spectrum disorder, Benign rolandic epilepsy (BRE), and cognitive impairments (1,2).

References

1. Duan W, Zhang YP, Hou Z, Huang C, Zhu H, Zhang CQ, Yin Q. (2016) Novel Insights into NeuN: from Neuronal Marker to Splicing Regulator. Mol Neurobiol. 53(3):1637-1647. PMID: 25680637.

2. Su CH, D D, Tarn WY. (2018) Alternative Splicing in Neurogenesis and Brain Development. Front Mol Biosci. 5:12. PMID: 29484299

Long Name

RNA binding fox-1 homolog 3

Alternate Names

FOX-3, FOX3, HRNBP3, NEUN

Gene Symbol

RBFOX3

Additional RBFOX3/NeuN Products

Product Documents for RBFOX3/NeuN Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RBFOX3/NeuN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RBFOX3/NeuN Antibody - BSA Free

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Protocols

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