RBM14 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-2884
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 550 and 600 of human CoActivator Activator (RNA Binding Motif 14) using the numbering given in entry NP_006319.1 (GeneID 10432 ).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RBM14 Antibody - BSA Free
Western Blot: RBM14 Antibody [NB100-2884]
Western Blot: RBM14 Antibody [NB100-2884] - Detection of Human and Mouse CoAA by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), Jurkat (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-CoAA antibody NB100-2884 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Immunoprecipitation: RBM14 Antibody [NB100-2884]
Immunoprecipitation: RBM14 Antibody [NB100-2884] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CoAA antibody used for IP at 6 ug/mg lysate. CoAA was also Immunoprecipitated by a previous lot this antibody. For blotting immunoprecipitated CoAA, NB100-2884 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for RBM14 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:1000-1:5000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RBM14
Alternate Names
COAAMGC31756, Paraspeckle protein 2, PSP2DKFZp779J0927, RNA binding motif protein 14, RNA-binding motif protein 14, RNA-binding protein 14, RRM-containing coactivator activator/modulator, SIPMGC15912, Synaptotagmin-interacting protein, SYT-interacting protein, SYTIP1, TMEM137, transmembrane protein 137
Entrez Gene IDs
10432 (Human)
Gene Symbol
RBM14
UniProt
Additional RBM14 Products
Product Documents for RBM14 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RBM14 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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