RBM5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83304

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RBM5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DKRVSRTERSGRYGSIIDRDDRDERESRSRRRDSDYKRSSDDRRGDRYDDYRDYDSPERERERRNSDRSEDGYHSDGDYGEHDYRHDISDERESKTIMLRGLPITITESDIREMMESFEGPQPADVRLMKRKTGVSRGFAFVEFYHL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RBM5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RBM5 Antibody [NBP1-83304]

Immunocytochemistry/ Immunofluorescence: RBM5 Antibody [NBP1-83304]

Immunocytochemistry/Immunofluorescence: RBM5 Antibody [NBP1-83304] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
RBM5 Antibody - BSA Free

RBM5 Antibody [NBP1-83304] -

Staining of human skin shows strong nuclear positivity in squamous epithelial cells.
RBM5 Antibody - BSA Free Western Blot: RBM5 Antibody - BSA Free [NBP1-83304]

Western Blot: RBM5 Antibody - BSA Free [NBP1-83304]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
RBM5 Antibody - BSA Free

RBM5 Antibody [NBP1-83304] -

Staining of human lymph node shows strong nuclear positivity in germinal center cells.
RBM5 Antibody - BSA Free

RBM5 Antibody [NBP1-83304] -

Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
RBM5 Antibody - BSA Free

RBM5 Antibody [NBP1-83304] -

Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.

Applications for RBM5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBM5

RNA-binding motif protein 5 (RBM5) is also known as LUCA-15. The RBM5 gene is located within a region highly susceptible to deletions in lung cancer and is implicated to be a tumor suppressor. RBM5 bears two RRM domains, one RanBP2-type zinc finger, one C2H2-type zinc finger, and a G-patch domain. It has been found to be a component of the spliceosome, a complex that regulates alternative splicing and is involved in the regulation of proliferation and apoptosis.

Alternate Names

G15, H37, LUCA15FLJ39876, Protein G15, Putative tumor suppressor LUCA15, Renal carcinoma antigen NY-REN-9, RMB5, RNA binding motif protein 5, RNA-binding motif protein 5, RNA-binding protein 5

Gene Symbol

RBM5

Additional RBM5 Products

Product Documents for RBM5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RBM5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RBM5 Antibody - BSA Free

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Protocols

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FAQs

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