RCC2 Antibody

Novus Biologicals | Catalog # NB110-40619

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation (Negative)

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RCC2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 475 and the C-terminus (residue 522) of human Regulator of Chromosome Condensation 2 using the numbering given in entry NP_061185.1 (GeneID 55920).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RCC2 Antibody

Western Blot: RCC2 Antibody [NB110-40619]

Western Blot: RCC2 Antibody [NB110-40619]

Western Blot: RCC2 Antibody [NB110-40619] - Detection of both input and immunoprecipitated RCC2 on KYSE-30 and KYSE-450 cell lines. Western blot image submitted by a verified customer review.
Immunohistochemistry-Paraffin: RCC2 Antibody [NB110-40619]

Immunohistochemistry-Paraffin: RCC2 Antibody [NB110-40619]

Immunohistochemistry-Paraffin: RCC2 Antibody [NB110-40619] - RCC2 expression from esophageal cancer tissue from mice. IHC-P image submitted by a verified customer review.
Western Blot: RCC2 Antibody [NB110-40619]

Western Blot: RCC2 Antibody [NB110-40619]

Western Blot: RCC2 Antibody [NB110-40619] - Detection of Human and Mouse RCC2 on HeLa whole cell lysate using NB110-40619. RCC2 was immunoprecipitated by rabbit anti-RCC2 antibody NB110-40618.
Immunohistochemistry-Paraffin: RCC2 Antibody [NB110-40619]

Immunohistochemistry-Paraffin: RCC2 Antibody [NB110-40619]

Immunohistochemistry-Paraffin: RCC2 Antibody [NB110-40619] - Sections of human skin basal cell carcinoma (left) and mouse CT26 colon carcinoma (right). Affinity purified rabbit anti- RCC2 used at a dilution of 1:1000 (0.2 ug/mL) and 1:200 (1 ug/mL). Detection: DAB

Applications for RCC2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. RCC2 antibody validated for IHC-P, WB from verified customer reviews.

Reviewed Applications

Read 2 reviews rated 5 using NB110-40619 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RCC2

RCC2 (regulator of chromosome condensation 2) is a member of the RCC1 family of guanine nucleotide exchange factors. RCC2 has been identified as part of the chromosomal passenger complex (CPC) which coordinates the chromosomal and cytoskeletal events of mitosis. Chromosomal passengers are proteins present in complexes with aurora B, inner centromeric protein (INCENP), survivin, and borealin. RCC2 has been demonstrated to be required for the recruitment of survivin and aurora B and is essential for proper chromosome segregation and the completion of cell cleavage. Loss of RCC2 inhibits spindle assembly, activates the spindle assembly checkpoint, and blocks cells in prometaphase. RCC2 is also known as telophase disk protein of 60 kDa, RCC1-like protein TD-60, TD60, KIAA1470, and DKFZp762N0610.

Alternate Names

DKFZp762N0610, KIAA1470, protein RCC2, RCC1-like protein TD-60, regulator of chromosome condensation 2, TD60, TD-60, Telophase disk protein of 60 kDa

Entrez Gene IDs

55920 (Human); 108911 (Mouse)

Gene Symbol

RCC2

UniProt

NP_061185.1 (Human)

Additional RCC2 Products

Product Documents for RCC2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RCC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for RCC2 Antibody (2)

5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • RCC2 Antibody
    Name: Ali Calderon Aparicio
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human Esophagus tissue
    Species: Mouse
    Verified Customer | Posted 11/04/2020
    Figure legend: RCC2 expression from esophageal cancer tissue from mice.
    Antigen retrieval with citrate buffer pH 6
    RCC2 Antibody NB110-40619
  • RCC2 Antibody
    Name: Ali Calderon
    Application: Western Blot
    Sample Tested: 293T cell line and Cancer Cell lysate
    Species: Human
    Verified Customer | Posted 03/19/2019
    Detection of RCC2 (IP RCC2) and Input.
    RCC2 Antibody NB110-40619

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Protocols

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FAQs

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