Recombinant Canine CD4 Protein, CF

Name: Recombinant Canine CD4 Protein, CF
Brand: R&D Systems
SKU: 9646-CD
Price: 731 USD
Availability: InStock

R&D Systems | Catalog # 9646-CD

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Product Documents

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

R&D Systems NS0-derived Recombinant Canine CD4 Protein (9646-CD)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P33705

View all CD4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived canine CD4 protein
Val25-Lys401, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val25

Predicted Molecular Mass

43 kDa

SDS-PAGE

42-61 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

9646-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS. Loading...
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes (1). CD4 is a standard phenotype marker for the identification of T cell populations (1). CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes (2, 3). CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV (4). Mature canine CD4 consists of a 377 aa extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain (5). Within the extracellular region, canine CD4 shares 57%, 46%, and 42% amino acid sequence identity with human, mouse, and rat CD4, respectively.