Recombinant Cynomolgus DNAM-1/CD226 Fc Chimera Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey DNAM-1/CD226 Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) that produces 50% of the optimal binding response is approximately
    0.2-1 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived
    Cynomolgus Monkey DNAM-1/CD226
    (Glu19-Asn247)
    Accession # XP_005586537
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Glu19
  • Predicted Molecular Mass
    53 kDa
  • SDS-PAGE
    74-87 kDa, reducing conditions
9276-DN
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Cynomolgus Monkey DNAM-1/CD226 Fc Chimera(Catalog # 9276-DN) is immobilized at 0.25 μg/mL, 100 μL/well, Recombinant Human CD155/PVR FcChimera (Catalog # 9174-CD) binds with a typical ED50 of
0.2-1 μg/mL.
Background: DNAM-1/CD226
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature cynomolgus DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, cynomolgus DNAM-1 shares 93%, 53% and 50% aa sequence identity with human, mouse, and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid, myeloid cells, and activated vascular endothelial cells and interacts with CD155/PVR and Nectin-2/CD112 (3-5). It competes with CD96 and TIGIT for binding to these proteins (6). It associates in cis with Integrin beta 2/CD18 on activated, but not resting, NK, T, and mast cells (5, 7, 8). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (2-4, 7, 9), Th17 polarization (10), dendritic cell maturation, megakaryocyte, and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it also inhibits the formation of osteoclasts (11-14). Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung (15). Downregulation of DNAM-1 on tumor cells can interfere with anti-tumor cellular immunity (16).
  • References:
    1. Ceboni, C. et al. (2013) Front. Immunol. 4:508.
    2. Shibuya, A. et al. (1996) Immunity 4:573.
    3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
    4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
    5. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
    6. Chan, C.J. et al. (2014) Nat. Immunol. 15:431.
    7. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
    8. Shibuya, K. et al. (1999) Immunity 11:615.
    9. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
    10. Lozano, E. et al. (2013) J. Immunol. 191:3673.
    11. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
    12. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
    13. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
    14. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
    15. Morimoto, K. et al. (2007) Oncogene 27:264.
    16. Carlsten, M. et al. (2009) J. Immunol. 183:4921.
  • Long Name:
    DNAX Accessory Molecule 1
  • Entrez Gene IDs:
    10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
  • Alternate Names:
    CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1/CD226; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1
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