Recombinant Cynomolgus/Rhesus Macaque B7-1/CD80 Protein, CF

Fc Chimera
Catalog # Availability Size / Price Qty
9695-B1-100
Recombinant Cynomolgus/Rhesus Macaque B7-1/CD80 Protein, CF SDS-PAGE
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Recombinant Cynomolgus/Rhesus Macaque B7-1/CD80 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED50 for this effect is 0.75-4.5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived B7-1/CD80 protein
Cynomolgus Monkey B7-1/CD80
(Val35-Asn242)
Accession # XP_005548122
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Val35
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51 kDa
SDS-PAGE
53-79 kDa, reducing conditions

Product Datasheets

9695-B1

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE Recombinant Cynomolgus/Rhesus Macaque B7-1/CD80 Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey B7‑1/CD80 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 53-79 kDa and 105-160 kDa, respectively.

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Background: B7-1/CD80

B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses (1). Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100-fold higher affinity than CD28 and is involved in the down-regulation of the immune response (2-5). B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells (2). Mature cynomolgus monkey B7-1 consists of a 213 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 22 aa transmembrane domain, and a 20 aa cytoplasmic domain. Within the ECD, cynomolgus monkey B7-1 shares 97% and 51% aa sequence identity with human and mouse B7-1, respectively. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas (6).

References
  1. Ville, S. et al. (2015) Front. Immunol. 6:411.
  2. Azuma, M. et al. (1993) Nature 366:76.
  3. Freeman, G.J. et al. (1993) Science 262:909.
  4. Chen, C. et al. (1994) J. Immunol. 152:4929.
  5. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
  6. Mir, M.A., et al. (2008) Expert Opin. Ther. Targets 12:969.
Entrez Gene IDs
941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
Alternate Names
Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80

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