Recombinant Cynomolgus/Rhesus Macaque CD19 Fc Protein, CF

R&D Systems | Catalog # 9958-CD

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Cynomolgus/Rhesus Macaque CD19 Fc Protein (9958-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

XP_005591597

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CD19 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived CD19 protein
Cynomolgus Monkey CD19
(Pro20-Lys292)
Accession # XP_005591597		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro20

Predicted Molecular Mass

57 kDa

SDS-PAGE

70-100 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey CD19 Fc Chimera is immobilized at 2 µg/mL,100 µL/well, recombinant biotinylated human CD81 Fc Chimera binds with an ED50 of 10-50 μg/mL.

Reviewed Applications

Read 7 reviews rated 5 using 9958-CD in the following applications:

Scientific Data Images for Recombinant Cynomolgus/Rhesus Macaque CD19 Fc Protein, CF

Recombinant CynomolgusMonkey/Rhesus Macaque CD19Fc Chimera Protein SEC-MALS.

Recombinant Cynomolgus Monkey/Rhesus Macaque CD19 Fc Chimera Protein (Catalog # 9958-CD) has a molecular weight (MW) of 252-278 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Cynomolgus/Rhesus Macaque CD19 Fc Protein SDS-PAGE

Recombinant Cynomolgus/Rhesus Macaque CD19 Fc Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey CD19 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 86-99 kDa and 170-190 kDa, respectively.

Formulation, Preparation, and Storage

9958-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD19

CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses (1, 2). CD19 is expressed throughout B cell development from pre-B cells through mature B cells, and it is commonly used as a B cell lineage marker (1). It is required for the responsiveness of mature B cell to antigen stimulation, germinal center development, and antibody affinity maturation (4, 5). CD19 associates with the B cell antigen receptor (BCR), CD81, CD38, CD21, CD22, and IFITM1/CD225/Leu13, (6-9). These associations enable CD19 to amplify B cell signaling (7, 9-12) and reduce the threshold for antigen stimulation through the BCR (13). CD19 polymorphisms and up-regulation can lead to the development of autoimmunity by promoting autoantibody production (2). CD19 has emerged as promising therapeutic target for hematologic cancers and solid tumors. Immunotherapy using a chimeric antigen receptor (CAR) targeting CD19 has proven effective in many different cancers. The first CD19 CAR T cell therapies have been granted FDA approval for the treatment of B cell malignancies with several more in clinical trials. Mature human CD19 consists of a 272 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 22 aa transmembrane segment, and a 243 aa cytoplasmic domain (3). Within the ECD, human CD19 shares 57% amino acid sequence identity with mouse and rat CD19.

References

  1. Wang, K. et al. (2012) Exp. Hematol. Oncol. 1:36.
  2. Tedder, T.F. et al. (1997) Immunity 6:107.
  3. Tedder, T.F. and C.M. Isaacs (1989) J. Immunol. 143:712.
  4. Rickert, R.C. et al. (1995) Nature 376:352.
  5. Engel, P. et al. (1995) Immunity 3:39.
  6. Vences-Catalan, F. et al. (2012) J. Immunol. 137:48.
  7. Deaglio, S. et al. (2007) Blood 109:5390.
  8. Bradbury, L. et al. (1992) J. Immunol. 149:2841.
  9. Krop, I. et al. (1996) J. Immunol. 157:48.
  10. O'Rourke, L.M. et al. (1998) Immunity 8:635.
  11. Buhl, A.M. et al. (1997) J. Exp. Med. 186:1897.
  12. Sato, S. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11558.
  13. Carter, R.H. and D.T. Fearon (1992) Science 256:105.

Alternate Names

CD19, CVID3, Leu-12

Entrez Gene IDs

930 (Human); 12478 (Mouse); 365367 (Rat); 397669 (Porcine); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)

Gene Symbol

CD19

UniProt

XP_005591597

Additional CD19 Products

Product Documents for Recombinant Cynomolgus/Rhesus Macaque CD19 Fc Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Cynomolgus/Rhesus Macaque CD19 Fc Protein, CF

For research use only

Customer Reviews for Recombinant Cynomolgus/Rhesus Macaque CD19 Fc Protein, CF (7)

5 out of 5
7 Customer Ratings
5 Stars
100%
4 Stars
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3 Stars
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2 Stars
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1 Stars
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Showing  1 - 5 of 7 reviews Showing All
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  • Recombinant Cynomolgus/Rhesus Macaque CD19 Fc Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 07/22/2025
  • Recombinant Cynomolgus/Rhesus Macaque CD19 Fc Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 05/07/2025
  • Name: Jenna Nguyen
    Application: Cell migration/motility
    Verified Customer | Posted 02/08/2019
  • Name: Jenna Nguyen
    Application: Cell Adhesion
    Verified Customer | Posted 01/30/2019
  • Name: Jenna Nguyen
    Application: In vivo study
    Verified Customer | Posted 01/30/2019
  • Recombinant Cynomolgus/Rhesus Macaque CD19 Fc Protein, CF
    Name: Jenna Nguyen
    Application: In vivo study
    Verified Customer | Posted 12/06/2018
  • Recombinant Cynomolgus/Rhesus Macaque CD19 Fc Protein, CF
    Name: Jenna Nguyen
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 11/05/2018

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