Recombinant Cynomolgus/Rhesus SLAM/CD150 Fc Protein, CF

Fc Chimera
Catalog # Availability Size / Price Qty
11170-SL-050
Recombinant Cynomolgus Monkey/Rhesus Macaque SLAM/CD150 Fc Chimera Protein SDS-PAGE.
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Recombinant Cynomolgus/Rhesus SLAM/CD150 Fc Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED50 for this effect is 0.200-2.00 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived SLAM/CD150 protein
Cynomolgus Monkey/Rhesus Macaque SLAM/CD150
(Tyr23-Lys236)
Accession # XP_005541298.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Tyr23 & Thr25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51 kDa
SDS-PAGE
60-80 kDa, under reducing conditions.

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11170-SL

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11170-SL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE Recombinant Cynomolgus Monkey/Rhesus Macaque SLAM/CD150 Fc Chimera Protein SDS-PAGE. View Larger

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque SLAM/CD150 Fc Chimera Protein (Catalog # 11170-SL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-80 kDa and 120-160 kDa, respectively.

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Background: SLAM/CD150

Signaling lymphocytic activation molecule (SLAM), also known as SLAMF1 and CD150, is the founding member of the SLAM subfamily of the CD2 protein family (1, 2). SLAM is a single-pass type I membrane glycoprotein that functions as an adhesion molecule and plays an active role in the regulation of innate and adaptive immunity (1, 2, 4). Mature SLAM consists of an extracellular domain (ECD) containing an Ig-like V-type domain and an Ig-like C2-type domain, a helical transmembrane domain, and a cytoplasmic tail containing 2 immunoreceptor tyrosine-based switch motifs (ITSM) (3, 4). The ECD of cynomolgus SLAM shares 97% amino acid identity with human SLAM. In human, several isoforms resulting from alternative splicing have been identified with functional diversity (4). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells, and it is up-regulated on activated B cells and CD4+ and CD8+ T cells (4-6). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 phenotype in CD8+ T cells that is characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity (7, 8). SLAM also plays a role in activation of the PI3K-Akt signaling pathway through its association with the adapter molecule SAP (9). In humans, SLAM functions as a cellular entry receptor for measles virus (10, 11). SLAM deregulation is associated with genomic complexity and independently predicts a worse outcome in chronic lymphocytic leukemia (CLL) (12).

References
  1. Yurchenko, M. et al. (2018) J. Cell. Biol. 217:1411.
  2. Pellegrini, J. et al. (2021) Autophagy. 17:2629.
  3. Wang, N. et al. (2015) Front. Immunol. 6:158.
  4. Gordiienko, I. (2019) Clinical Immunol. 204:14.
  5. Calpe, S. et al. (2008) Adv Immunol. 97:177.
  6. Wang, N. et al. (2004) J. Exp. Med. 199:1255.
  7. Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100.
  8. Mehrle, S. et al. (2008) Mol. Immunol. 45:796.
  9. Yurchenko, M.Y. et al. (2005) Exp Oncol. 27:24.
  10. Hsu, E.C. et al. (2001) Virology 279:9.
  11. Gonçalves-Carneiro, D. et al. (2017) J Virol. 91:e02255.
  12. Gian, M.R. et al. (2021) Br. J. Haematol. 192:1068.
Long Name
Signaling Lymphocytic Activation Molecule
Entrez Gene IDs
6504 (Human); 27218 (Mouse); 102135470 (Cynomolgus Monkey)
Alternate Names
CD 150; CD150; CD150IPO-3; CDw150; IPO-3; signaling lymphocytic activation molecule family member 1; signaling lymphocytic activation molecule; SLAM; SLAMCD150 antigen; SLAMF1

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