Recombinant Cynomolgus/Rhesus SLAM/CD150 His-tag Protein, CF Summary
Tyr23-Lys236, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED50 for this effect is 0.500-2.50 μg/mL.
2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque SLAM/CD150 His-tag Protein (Catalog # 10971-SL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 42-55 kDa.
Signaling lymphocytic activation molecule family member 1 (SLAMF1), also known as SLAM and CD150, is the founding member of the SLAM subfamily of the CD2 protein family (1, 2). SLAM is a single-pass type I membrane glycoprotein that functions as an adhesion molecule and plays an active role in the regulation of innate and adaptive immunity (1, 2, 4). Mature SLAM consists of an extracellular domain (ECD) containing an Ig-like V-type domain and an Ig-like C2-type domain, a helical transmembrane, and a cytoplasmic tail containing an immunoreceptor tyrosine-based switch motif (ITSM 1), a SH2-binding motif and an ITSM 2 (3, 4). In human, several isoforms resulting from alternative splicing have been identified (4). The ECD of cynomolgus SLAM shares 97% amino acid identity with human SLAM. SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells, and it is up-regulated on activated B cells and CD4+ and CD8+ T cells (4-6). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 phenotype in CD8+ T cells that is characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity (7, 8). SLAM also plays a role in activation of the PI3K‑Akt signaling pathway through its association with the adapter molecule SAP (9). In humans, SLAM functions as a cellular entry receptor for measles virus (10, 11). SLAM deregulation is associated with genomic complexity and independently predicts a worse outcome in chronic lymphocytic leukaemia (CLL) (12).
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