Recombinant HCoV-OC43 Spike His-tag Protein, CF

R&D Systems | Catalog # 10989-CV

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems HEK293-derived Recombinant HCoV-OC43 Spike His-tag Protein (10989-CV)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

YP_009555241.1

Applications

Bioactivity
View all Spike Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived hcov-oc43 Spike protein
Val15-Pro1297 (Arg754Ser, Arg757Ser), with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val15

Predicted Molecular Mass

144 kDa

SDS-PAGE

>150 kDa, under reducing conditions.

Activity

Measured by its ability to agglutinate mouse red blood cells. The ED50 for this effect is 0.10‑1.00 µg/mL.

Scientific Data Images for Recombinant HCoV-OC43 Spike His-tag Protein, CF

Recombinant HCoV-OC43 Spike His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant HCoV-OC43 Spike His-tag Protein (Catalog # 10989-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at >150 kDa.

Formulation, Preparation, and Storage

10989-CV
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Spike

HCoV-OC43, a virus first isolated in 1960's that accounts for ~ 20% of the common cold, belongs to a family of viruses known as coronaviruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1, 2). Other well-known human coronaviruses include three viruses that cause relatively mild respiratory disease: HCoV-229E, HCoV-HKU1 and HCov-NL63, plus three viruses that cause the Severe Acute Respiratory Syndrome (SARS-CoV), the Middle East Respirator Syndrome (MERS-CoV), and the global pandemic Covid-19 (SARS-CoV2). While the S, E and M proteins build up the viral envelop, the N protein is involved transcription, replication, and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (3, 4). The CoV-OC43 N protein is a ~50 kDa protein composed of two independent structural domains connected by a linker region. Both the N-terminal and the linker regions contain RNA binding domains, while the C-terminal region is responsible for the oligomerization of the N protein (5). The CoV‑OC43 N protein shares 64% amino acid sequence identity with CoV-HKU1 N protein. the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity. Cross activity of antibodies among different strains should be rigorously tested when designing serological diagnostic kits (6, 7).

References

  1. St-Jean, J.R. et al. (2004) J. Virol. 78:8824.
  2. Vabret, A. et al. (2003) Fr Clin Infect Dis. 36:985.
  3. Chang, C.K. et al. (2006) J. Biomed. Sci. 13:59.
  4. Hurst, K.R. et al. (2009) J. Virol. 83:7221.
  5. Huang, C.Y. et al. (2009) Protein Sci. 18:2209.
  6. Chan, K.H. et al. (2005) Clin Diagn Lab Immunol. 12:1317.
  7. Mourez, T. et al. (2007) J. Virol Methods. 139:175.

Long Name

Spike Protein

Alternate Names

S Protein

Entrez Gene IDs

918758 (HCoV-229E); 2943499 (HCoV-NL63); 39105218 (HCoV-OC43); 37616432 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)

Gene Symbol

S

UniProt

YP_009555241.1

Additional Spike Products

Product Documents for Recombinant HCoV-OC43 Spike His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant HCoV-OC43 Spike His-tag Protein, CF

For research use only

Related Research Areas

Infectious Diseases

Customer Reviews for Recombinant HCoV-OC43 Spike His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant HCoV-OC43 Spike His-tag Protein, CF and earn rewards!

Have you used Recombinant HCoV-OC43 Spike His-tag Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes