Recombinant Human Aldo-keto Reductase 1B10/AKR1B10, CF

R&D Systems | Catalog # 7529-DH

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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human Aldo-keto Reductase 1B10/AKR1B10 (7529-DH)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

O60218

Applications

Enzyme Activity
View all Aldo-keto Reductase 1B10/AKR1B10 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Aldo-keto Reductase 1B10/AKR1B10 protein
Ala2-Tyr316, with an N-terminal Met and 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments

Predicted Molecular Mass

37 kDa

SDS-PAGE

37-38 kDa, reducing conditions

Activity

Measured by the disappearance of NADPH during the reducation of 4-nitrobenzaldehyde.
The specific activity is >1,000 pmol/min/μg, as measured under the described conditions.

Reviewed Applications

Read 2 reviews rated 5 using 7529-DH in the following applications:

Formulation, Preparation, and Storage

7529-DH
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Aldo-keto Reductase 1B10/AKR1B10

Aldo-keto reductase family 1 member B10 (AKR1B10), also known as ARL-1 or aldose reductase related protein, is a cytosolic, NADPH-dependent oxidoreductase that reduces variety of aromatic and aliphatic aldehydes, dicarbonyl compounds, and some drug ketones (1). It is related to AKR1B1 in amino acid sequence and tertiary structure. However, its substrate specificity differs from AKR1B1 in its inability to reduce sugars and prostaglandin H2, and in its high catalytic efficiency for retinals, isoprenyl aldehydes, and cytotoxic aldehydes (2). It is primarily observed in the human colon, small intestine, and adrenal gland, with a low level in liver. Its up‑regulated expression is detected in hepatocellular carcinoma, cervical cancer, lung squamous cell carcinoma, and lung adenocarcinoma in smokers (3). Thus, it is considered as a potential diagnostic and/or prognostic marker in carcinomas and serum. The functional studies of this enzyme suggest that the up‑regulation of AKR1B10 is related to cell survival by reducing chemo-agents or metabolites in cells to less toxic reduced forms (4). Its inhibition may be a potential target for reducing the progression of cancers (5).

References

  1. Endo, S. et al. (2009) Arch. Biochem. Biophys. 487:1.
  2. Gallego, O. et al. (2007) Proc. Natl. Acad. Sci. USA 104:20764.
  3. Matsunaga, T. et al. (2012) Front. Pharmacol. 3:1.
  4. Wang, C. et al. (2009) J. Biol. Chem. 284:26742.
  5. Matsunaga, T. et al. (2011) Anticancer Drugs 22:402.

Long Name

Aldo-keto Reductase Family 1, Member B10 [Aldose Reductase]

Alternate Names

AKR1B10, AKR1B11, AKR1B12, AldoketoReductase 1B10, ARL1, HIS, HSI

Entrez Gene IDs

57016 (Human); 67861 (Mouse); 296972 (Rat)

Gene Symbol

AKR1B10

UniProt

O60218

Additional Aldo-keto Reductase 1B10/AKR1B10 Products

Product Documents for Recombinant Human Aldo-keto Reductase 1B10/AKR1B10, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human Aldo-keto Reductase 1B10/AKR1B10, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

For research use only

Related Research Areas

Redox Enzymes

Customer Reviews for Recombinant Human Aldo-keto Reductase 1B10/AKR1B10, CF (2)

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    Name: Anonymous
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    Verified Customer | Posted 11/16/2018
    Recombinant Human Aldo-keto Reductase 1B10/AKR1B10, CF 7529-DH
  • Recombinant Human Aldo-keto Reductase 1B10/AKR1B10, CF
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 10/10/2018
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Protocols

View specific protocols for Recombinant Human Aldo-keto Reductase 1B10/AKR1B10, CF (7529-DH):

Materials
  • Assay Buffer: 50 mM Sodium Acetate, 0.1 M NaCl, pH 5.0
  • Recombinant Human Aldo‑keto Reductase 1B10/AKR1B10 (rhAKR1B10) (Catalog # 7529-DH)
  • beta -Nicotinamide adenine dinucleotide phosphate reduced, tetrasodium salt ( beta -NADPH) (Sigma, Catalog # N7505), 10 mM in deionized water
  • 4-nitro-benzaldehyde (Fluka, Catalog # 72800), 200 mM in DMSO
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhAKR1B10 to 4 ng/μL in Assay Buffer.
  2. Prepare a Reaction Mixture containing 2 mM 4-nitro-benzaldehyde and 400 μM beta -NADPH in Assay Buffer.
  3. In a plate, load 50 μL of 4 ng/μL rhAKR1B10, and start the reaction by adding 50 μL of Reaction Mixture.
  4. Include a Substrate Blank containing 50 μL of Assay Buffer and 50 μL of Reaction Mixture.
  5. Read at an absorbance of 340 nm in kinetic mode for 5 minutes.
  6. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (OD/min) x -1 x well volume (L) x 1012 pmol/mol
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

     *Adjusted for Substrate Blank 
     **Using the extinction coefficient 6270 M-1cm-1 
     ***Using the path correction 0.32 cm
     Note: the output of many spectrophotometers is in mOD Per Well:
  • rhAKR1B10: 0.2 μg
  • 4-nitro-benzaldehyde: 1 mM
  • beta -NADPH: 200 μM

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