Recombinant Human Angiopoietin-like 2 Protein, CF Summary
Gln23-His493, with a C-terminal 9-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CHAPS with Trehalose.|
|Reconstitution||Reconstitute at 200 μg/mL in water.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Angiopoietin-like Protein 2/ANGPTL2
Angiopoietin-like Protein 2 (ANGPTL2), also known as ARP2, is a secreted 56 kDa glycoprotein that contains a coiled coil domain and a fibrinogen like domain (1). ANGPTL2 is one of the eight members of the Angiopoietin like family proteins, which are structurally similar to angiopoietins (2). Mature human ANGPTL2 shares 89% sequence identity with both mouse and rat homologs. ANGPTL2 is widely expressed in many tissues, and its expression is induced by chronic, but not acute hypoxia (3). Elevated level of ANGPTL2 in the serum is also correlated with inflammation and obesity (3). ANGPTL2 has both pro and anti-angiogenic functions (1, 4). It serves as tumor suppressor in ovarian cancer (5), and also promote metastasis in various cancer types, making it potential biomarker for tumor progression (6, 7). ANGPTL2 functions as growth factor to enhance the survival of hematopoietic progenitors, mediated by its coiled-coil domain (8). Receptor of ANGPTL2 is recently identified to be LILRB2, and both the coiled-coil domain and fibrinogen like domain are required for optimal binding (9).
- Kim, I. et al. (1999), J Biol Chem 274:26523.
- Santulli, G. et al. (2014) Front Endocrinol 5:4.
- Tabata, M. et al. (2009) Cell Metab 10:178.
- Kubota, Y. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:13502.
- Kikuchi, R. et al. (2008) Cancer Res 68:5067.
- Odagiri, H. et al. (2014) Sci Signal 7:ra7.
- Endo, M. et al. (2012) Cancer Res 72:1784.
- Broxmeyer, H.E. et al. (2012) Blood Cells Mol Dis 48:25.
- Deng, M. et al. (2014) Blood 124:924.
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Human Angiopoietin-like 2 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Angiopoietin-like 2 Protein, CF and earn rewards!
Have you used Recombinant Human Angiopoietin-like 2 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image