Recombinant Human Angiopoietin-like 2 Protein, CF

Catalog # Availability Size / Price Qty
9746-AN-025/CF
Recombinant Human Angiopoietin-like 2 Protein Binding Activity
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Recombinant Human Angiopoietin-like 2 Protein, CF Summary

Product Specifications

Purity
>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Angiopoietin‑like Protein 2/ANGPTL2 is coated at 2 μg/mL, Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078‑T4) binds with an ED50 of 0.02‑0.12 μg/mL Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED50 for this effect is 0.3-1.8 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Angiopoietin-like Protein 2/ANGPTL2 protein
Gln23-His493, with a C-terminal 9-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln23 inferred from enzymatic pyroglutamate treatment revealing Glu24
Structure / Form
Disulfide-linked homooligomer
Predicted Molecular Mass
56 kDa
SDS-PAGE
60-69 kDa, reducing conditions

Product Datasheets

9746-AN/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9746-AN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CHAPS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Human Angiopoietin-like 2 Protein Binding Activity View Larger

When Recombinant Angiopoietin-like Protein 2/ANGPTL2 (Catalog # 9746-AN) is coated at 2 µg/mL, Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4) binds with an ED50 of 0.02-0.12 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 2/ANGPTL2

Angiopoietin-like Protein 2 (ANGPTL2), also known as ARP2, is a secreted 56 kDa glycoprotein that contains a coiled coil domain and a fibrinogen like domain (1). ANGPTL2 is one of the eight members of the Angiopoietin like family proteins, which are structurally similar to angiopoietins (2). Mature human ANGPTL2 shares 89% sequence identity with both mouse and rat homologs. ANGPTL2 is widely expressed in many tissues, and its expression is induced by chronic, but not acute hypoxia (3). Elevated level of ANGPTL2 in the serum is also correlated with inflammation and obesity (3). ANGPTL2 has both pro and anti-angiogenic functions (1, 4). It serves as tumor suppressor in ovarian cancer (5), and also promote metastasis in various cancer types, making it potential biomarker for tumor progression (6, 7). ANGPTL2 functions as growth factor to enhance the survival of hematopoietic progenitors, mediated by its coiled-coil domain (8). Receptor of ANGPTL2 is recently identified to be LILRB2, and both the coiled-coil domain and fibrinogen like domain are required for optimal binding (9).

References
  1. Kim, I. et al. (1999), J Biol Chem 274:26523.
  2. Santulli, G. et al. (2014) Front Endocrinol 5:4.
  3. Tabata, M. et al. (2009) Cell Metab 10:178.
  4. Kubota, Y. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:13502.
  5. Kikuchi, R. et al. (2008) Cancer Res 68:5067.
  6. Odagiri, H. et al. (2014) Sci Signal 7:ra7.
  7. Endo, M. et al. (2012) Cancer Res 72:1784.
  8. Broxmeyer, H.E. et al. (2012) Blood Cells Mol Dis 48:25.
  9. Deng, M. et al. (2014) Blood 124:924.
Entrez Gene IDs
23452 (Human); 26360 (Mouse)
Alternate Names
angiopoietin-like 2; Angiopoietin-like Protein 2; angiopoietin-related protein 2; ANGPTL2; ANGRP2; ARP2HARP; MGC8889

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