Recombinant Human Angiopoietin-like 4 C-Terminal Frag, CF

Catalog # Availability Size / Price Qty
3485-AN-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Angiopoietin-like 4 C-Terminal Frag, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Angiopoietin‑like 4/ANGPTL4 C‑Terminal Fragment is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera  (Catalog # 2078-T4) binds with an ED50 of 70.0-350 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Angiopoietin-like Protein 4/ANGPTL4 protein
Leu165-Ser406, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu165
Structure / Form
Oligomer
Predicted Molecular Mass
28 kDa
SDS-PAGE
36 kDa, reducing conditions

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3485-AN

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3485-AN

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Angiopoietin-like Protein 4/ANGPTL4

ANGPT-L4, also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue that is structurally related to the angiopoietins. It contains an N‑terminal coiled coil domain and a C‑terminal fibrinogen-like domain which can be proteolytically separated in vivo (1, 2). Mature human ANGPT‑L4 shares 26%‑30% amino acid (aa) sequence identity with ANGPT-L1, 2, 3, 5, 6, and 7. It shares approximately 75% aa sequence identity with mouse and rat ANGPT‑L4. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers (3, 4). This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels (5-9). In human, the N-terminal fragment and full length ANGPT-L4 physically associate with HDL (9). In mouse, however, full length ANGPT-L4 associates with HDL, while the N-terminal fragment associates with LDL (9). Circulating ANGPT-L4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity (10). Its expression in adipose tissue is induced by fasting and suppressed by feeding (3, 11). Its expression in both liver and fat is up‑regulated by PPAR alpha, beta, gamma, and δ agonists and down‑regulated by insulin (3, 12, 13). ANGPT-L4 is induced in vascular endothelial cells by hypoxia and in hypoxic areas surrounding tumors (14-16). The full length molecule but not the C-terminal fragment is bound by heparan sulfate proteoglycans and functions as an angiogenesis inhibitor (14, 15).

References
  1. Li, C. (2006) Curr. Opin. Lipidol. 17:152.
  2. Kersten, S. (2005) Biochem. Soc. Transact. 33:1059.
  3. Ge, H. et al. (2005) J. Lipid Res. 46:1484.
  4. Ge, H. et al. (2004) J. Biol. Chem. 279:2038.
  5. Sukonina, V. et al. (2006) Proc. Natl. Acad. Sci. 103:17450.
  6. Koster, A. et al. (2005) Endocrinology 146:4943.
  7. Yoshida, K. et al. (2002) J. Lipid Res. 43:1770.
  8. Ge, H. et al. (2004) J. Lipid Res. 45:2071.
  9. Mandard, S. et al. (2006) J. Biol. Chem. 281:934.
  10. Xu, A. et al. (2005) Proc. Natl. Acad. Sci. 102:6086.
  11. Kersten, S. et al. (2000) J. Biol. Chem. 275:28488.
  12. Mandard, S. et al. (2004) J. Biol. Chem. 279:34411.
  13. Yamada, T. et al. (2006) Biochem. Biophys. Res. Commun. 347:1138.
  14. Cazes, A. et al. (2006) Circ. Res. 99:1207.
  15. Le Jan, S. et al. (2003) Am. J. Pathol. 162:1521.
  16. Ito, Y. et al. (2003) Cancer Res. 63:6651.
Entrez Gene IDs
51129 (Human); 57875 (Mouse); 362850 (Rat)
Alternate Names
Angiopoietin like Protein 4; angiopoietin-like 4; Angiopoietin-like Protein 4; ANGPTL4; ARP4fasting-induced adipose factor; FIAF; FIAFhepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; HFARPANGPTL2; NL2; peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein; PGAR; PGARangiopoietin-related protein 4; pp1158; PPARG angiopoietin related protein

Citations for Recombinant Human Angiopoietin-like 4 C-Terminal Frag, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Plasma angiopoietin-like 4 is related to phospholipid transfer protein activity in diabetic and non-diabetic subjects: role of enhanced low grade inflammation
    Authors: EG Gruppen, S Kersten, RPF Dullaart
    Lipids Health Dis, 2018;17(1):60.
    Applications: ELISA (Standard)
  2. Effect of diet-induced weight loss on angiopoietin-like protein 4 and adipose tissue lipid metabolism in overweight and obese humans
    Authors: BW van der Ko, RG Vink, JWE Jocken, NJT Roumans, GH Goossens, ECM Mariman, MA van Baak, EE Blaak
    Physiol Rep, 2018;6(13):e13735.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA (Standard)
  3. Hypoxia-induced ANGPTL4 sustains tumour growth and anoikis resistance through different mechanisms in scirrhous gastric cancer cell lines
    Authors: K Baba, Y Kitajima, S Miyake, J Nakamura, K Wakiyama, H Sato, K Okuyama, H Kitagawa, T Tanaka, M Hiraki, K Yanagihara, H Noshiro
    Sci Rep, 2017;7(1):11127.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Short-chain fatty acids stimulate angiopoietin-like 4 synthesis in human colon adenocarcinoma cells by activating peroxisome proliferator-activated receptor gamma.
    Authors: Alex S, Lange K, Amolo T, Grinstead J, Haakonsson A, Szalowska E, Koppen A, Mudde K, Haenen D, Al-Lahham S, Roelofsen H, Houtman R, van der Burg B, Mandrup S, Bonvin A, Kalkhoven E, Muller M, Hooiveld G, Kersten S
    Mol Cell Biol, 2013;33(7):1303-16.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA (Standard)
  5. Caloric restriction and exercise increase plasma ANGPTL4 levels in humans via elevated free fatty acids.
    Authors: Kersten S, Lichtenstein L, Steenbergen E, Mudde K, Hendriks HF, Hesselink MK, Schrauwen P, Muller M
    Arterioscler. Thromb. Vasc. Biol., 2009;29(6):969-74.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)

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