Recombinant Human B7-H6 Fc Chimera Avi-tag Protein, CF

R&D Systems | Catalog # AVI7144

Biotinylated
R&D Systems
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human B7-H6 Fc Chimera Avi-tag Protein (AVI7144)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q68D85.1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity
View all B7-H6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human B7-H6 protein
Human B7-H6
(Asp25-Ser262)
Accession # Q68D85.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp25

Predicted Molecular Mass

55 kDa

SDS-PAGE

70-78 kDa, under reducing conditions

Activity

Measured by its ability to induce IFN-gamma secretion by NK‑92 human natural killer lymphoma cells. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
The ED50 for this effect is 0.6-3 μg/mL.

Measured by its binding ability in a functional ELISA.
When Recombinant Human NKp30 Fc Chimera Protein  (Catalog # 1849-NK) is immobilized at 0.5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human B7‑H6 Fc Chimera Avi-tag that produces 50% of the optimal binding response is found to be approximately 2-12 ng/mL.

Scientific Data Images for Recombinant Human B7-H6 Fc Chimera Avi-tag Protein, CF

When Recombinant Human NKp30 Fc Chimera Protein (1849-NK) is immobilized at 0.5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human B7‑H6 Fc Chimera Avi-tag (Catalog # AVI7144) that produces 50% of the optimal binding response is found to be approximately 2-12 ng/mL.

2 μg/lane of Biotinylated Recombinant Human B7-H6 Fc Chimera Avi-tag (Catalog # AVI7144) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-78 kDa and 130-150 kDa, respectively.

Formulation, Preparation, and Storage

AVI7144
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: B7-H6

B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins (1, 2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53% - 56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7). Our Avi-tag Biotinylated human B7-H6 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
  4. Li, Y. et al. (2011) J. Exp. Med. 208:703.
  5. Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. 108:6223.
  6. Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
  7. Byrd, A. et al. (2007) PLoS ONE 2:e1339.

Long Name

B7 Homolog 6

Alternate Names

B7H6, DKFZp686I21167, DKFZp686O24166, NCR3LG1

Entrez Gene IDs

374383 (Human); 102121659 (Cynomolgus Monkey)

Gene Symbol

NCR3LG1

UniProt

Q68D85.1

Additional B7-H6 Products

Product Documents for Recombinant Human B7-H6 Fc Chimera Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human B7-H6 Fc Chimera Avi-tag Protein, CF

For research use only

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