Recombinant Human BCMA/TNFRSF17 Fc Avi-tag Protein, CF

R&D Systems | Catalog # AVI193

Biotinylated
R&D Systems
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human BCMA/TNFRSF17 Fc Avi-tag Protein (AVI193)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q02223-1

Structure / Form

Disulfide-linked homodimer, biotinylated protein via Avi-tag

Conjugate

Biotin

Applications

Bioactivity
View all BCMA/TNFRSF17 Proteins and Enzymes »

Why choose R&D Systems Avi-tag BCMA Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human BCMA/TNFRSF17 protein
Human BCMA/TNFRSF17
(Met1-Ala54)
Accession # Q02223-1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

34 kDa

SDS-PAGE

36-48 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP) is immobilized at 0.1 µg/mL (100 µL/well), Recombinant Human BCMA/TNFRSF17 Fc Chimera Avi-tag (Catalog # AVI193) binds with an ED50 of 0.3-1.8 ng/mL.

Reviewed Applications

Read 1 review rated 5 using AVI193 in the following applications:

Scientific Data Images for Recombinant Human BCMA/TNFRSF17 Fc Avi-tag Protein, CF

Recombinant Human BCMA/TNFRSF17 Fc Avi-tag Protein Binding Activity

Recombinant Human BCMA/TNFRSF17 Fc Avi-tag Protein Binding Activity

When Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP) is immobilized at 0.1 µg/mL, Recombinant Human BCMA/TNFRSF17 Fc Chimera Avi-tag (Catalog # AVI193) binds with an ED50 of 0.3-1.8 ng/mL.
Recombinant Human BCMA/TNFRSF17 Fc Avi-tag Protein SDS-PAGE

Recombinant Human BCMA/TNFRSF17 Fc Avi-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human BCMA/TNFRSF17 Fc Chimera Avi-tag (Catalog # AVI193) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 36-48 kDa and 70-100 kDa, respectively.

Formulation, Preparation, and Storage

AVI193
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BCMA/TNFRSF17

BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

References

  1. Madry, C. et al. (1998) Int. Immunol. 10:1693.
  2. Gras, M. et al. (1995) Int. Immunol. 7:1093.
  3. Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
  4. Marsters, S. et al. (2000) Curr. Biol. 10:785.
  5. Thompson, J. et al. (2000) J. Exp. Med. 192:129.

Long Name

B Cell Maturation Factor

Alternate Names

CD269, TNFRSF13A, TNFRSF17

Entrez Gene IDs

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Gene Symbol

TNFRSF17

UniProt

Q02223-1

Additional BCMA/TNFRSF17 Products

Product Documents for Recombinant Human BCMA/TNFRSF17 Fc Avi-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human BCMA/TNFRSF17 Fc Avi-tag Protein, CF

For research use only

Customer Reviews for Recombinant Human BCMA/TNFRSF17 Fc Avi-tag Protein, CF (1)

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  • Very good protein
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 09/09/2024
    Blue color shows binding between AVI193 and 2149-BF
    This protein is bioactive and can be used for ELISA. I used it for ELISA with 5860-AP and 2149-BF (I have also provided their separate reviews). Initially, I didn't see any binding signal between AVI193 and 5860-AP, which made me to try AVI193 and 2149-BF. I eventually confirmed binding. In the attached image, I immobilized the protein (AVI193) on streptavidin-coated plate, and then added the ligands vs control. After watching with PBS, appropriate antibodies were used to detect binding.
    Recombinant Human BCMA/TNFRSF17 Fc Avi-tag Protein, CF AVI193

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