Bone morphogenetic protein 15 (BMP-15), also known as GDF-9B, is a TGF-beta superfamily ligand that is expressed by oocytes throughout folliculogenesis, and plays an important role in oocyte development (1). BMP-15 promotes the FSH‑independent proliferation of ovarian granulosa cells (GC) and induces GC glycolysis and cholesterol synthesis (2‑4). It also induces GC production of stem cell factor which, in turn, negatively regulates BMP-15 expression in oocytes (5). BMP-15 blocks the FSH‑induced GC expression of FSH R and multiple steroidogenic molecules (6). BMP-15 is synthesized with a 249 amino acid (aa) N-terminal propeptide (7). The propeptide is cleaved intracellularly from the 50 kDa proBMP-15 but remains associated with mature BMP-15 (8). Mature BMP-15 exists in 16 kDa and 17 kDa forms which are distinguishable by the presence of O-linked glycosylation on the 17 kDa form (8). Mature BMP-15 is phosphorylated, a modification which is required for the stimulation of GC proliferation (9). BMP-15 exerts its effects through interactions with BMPR‑IB/ALK6 and BMPR‑II (9-11). Mature BMP-15 forms 34 kDa noncovalently-linked homodimers and 37 kDa heterodimers with mature GDF-9 (12). Both of these oocyte-expressed factors lack the cysteine that mediates disulfide-linked dimerization in most TGF-beta superfamily ligands (1). Although heterodimerization with GDF-9 may limit the secretion of active BMP‑15, these two factors synergize in promoting oocyte survival and folliculogenesis (12, 13). Mature human BMP-15 shares 70%, 68%, and 78% aa sequence identity with mouse, rat, and sheep BMP-15, respectively. It shares 27%-38% aa sequence with other BMPs.
Recombinant Human BMP-15 Protein
R&D Systems | Catalog # 5096-BM
Key Product Details
- R&D Systems CHO-derived Recombinant Human BMP-15 Protein (5096-BM)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
Gln268-Arg392 (Thr266Arg)
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Approximately 10 ng/mL can effectively induce Smad3 phosphorylation.
Reviewed Applications
Read 2 reviews rated 4 using 5096-BM in the following applications:
Formulation, Preparation, and Storage
5096-BM
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: BMP-15/GDF-9B
References
- Moore, R.K. and S. Shimasaki (2005) Mol. Cell. Endocrinol. 234:67.
- Otsuka, F. et al. (2000) J. Biol. Chem. 275:39523.
- Sugiura, K. et al. (2007) Development 134:2593.
- Su, Y.-Q. et al. (2008) Development 135:111.
- Otsuka, F. and S. Shimasaki (2002) Proc. Natl. Acad. Sci. 99:8060.
- Otsuka, F. et al. (2001) J. Biol. Chem. 276:11387.
- Dube, J.L. et al. (1998) Mol. Endocrinol. 12:1809.
- Saito, S. et al. (2008) Prot. Sci. 17:362.
- McMahon, H.E. et al. (2008) Endocrinology 149:812.
- Moore, R.K. et al. (2003) J. Biol. Chem. 278:304.
- Edwards, S.J. et al. (2008) Endocrinology 149:1026.
- Liao, W.X. et al. (2003) J. Biol. Chem. 278:3713.
- Yan, C. et al. (2001) Mol. Endocrinol. 15:854.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional BMP-15/GDF-9B Products
Product Documents for Recombinant Human BMP-15 Protein
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human BMP-15 Protein
For research use only
Related Research Areas
Citations for Recombinant Human BMP-15 Protein
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 02/18/2021
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Application: Cell ProliferationVerified Customer | Posted 10/09/2020
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FAQs for Recombinant Human BMP-15 Protein
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Q: What is the half-life of this BMP protein?
A: R&D Systems has not determined the half-life of this recombinant human BMP protein. Assay conditions for each protein will need to be optimized by each researcher.