Recombinant Human BMP-15 Protein

Catalog # Availability Size / Price Qty
5096-BM-005
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (19)
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Reviews (2)

Recombinant Human BMP-15 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce Smad3 phosphorylation in P19 mouse embryonal carcinoma cells. Approximately 10 ng/mL can effectively induce Smad3 phosphorylation.
Source
Chinese Hamster Ovary cell line, CHO-derived human BMP-15/GDF-9B protein
Gln268-Arg392 (Thr266Arg)
Accession #
N-terminal Sequence
Analysis
Gln268
Predicted Molecular Mass
13.9 kDa
SDS-PAGE
15 kDa, reducing conditions

Product Datasheets

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5096-BM

5096-BM

Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4  mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: BMP-15/GDF-9B

Bone morphogenetic protein 15 (BMP-15), also known as GDF-9B, is a TGF-beta superfamily ligand that is expressed by oocytes throughout folliculogenesis, and plays an important role in oocyte development (1). BMP-15 promotes the FSH‑independent proliferation of ovarian granulosa cells (GC) and induces GC glycolysis and cholesterol synthesis (2‑4). It also induces GC production of stem cell factor which, in turn, negatively regulates BMP-15 expression in oocytes (5). BMP-15 blocks the FSH‑induced GC expression of FSH R and multiple steroidogenic molecules (6). BMP-15 is synthesized with a 249 amino acid (aa) N-terminal propeptide (7). The propeptide is cleaved intracellularly from the 50 kDa proBMP-15 but remains associated with mature BMP-15 (8). Mature BMP-15 exists in 16 kDa and 17 kDa forms which are distinguishable by the presence of O-linked glycosylation on the 17 kDa form (8). Mature BMP-15 is phosphorylated, a modification which is required for the stimulation of GC proliferation (9). BMP-15 exerts its effects through interactions with BMPR‑IB/ALK6 and BMPR‑II (9-11). Mature BMP-15 forms 34 kDa noncovalently-linked homodimers and 37 kDa heterodimers with mature GDF-9 (12). Both of these oocyte-expressed factors lack the cysteine that mediates disulfide-linked dimerization in most TGF-beta superfamily ligands (1). Although heterodimerization with GDF-9 may limit the secretion of active BMP‑15, these two factors synergize in promoting oocyte survival and folliculogenesis (12, 13). Mature human BMP-15 shares 70%, 68%, and 78% aa sequence identity with mouse, rat, and sheep BMP-15, respectively. It shares 27%-38% aa sequence with other BMPs.

References
  1. Moore, R.K. and S. Shimasaki (2005) Mol. Cell. Endocrinol. 234:67.
  2. Otsuka, F. et al. (2000) J. Biol. Chem. 275:39523.
  3. Sugiura, K. et al. (2007) Development 134:2593.
  4. Su, Y.-Q. et al. (2008) Development 135:111.
  5. Otsuka, F. and S. Shimasaki (2002) Proc. Natl. Acad. Sci. 99:8060.
  6. Otsuka, F. et al. (2001) J. Biol. Chem. 276:11387.
  7. Dube, J.L. et al. (1998) Mol. Endocrinol. 12:1809.
  8. Saito, S. et al. (2008) Prot. Sci. 17:362.
  9. McMahon, H.E. et al. (2008) Endocrinology 149:812.
  10. Moore, R.K. et al. (2003) J. Biol. Chem. 278:304.
  11. Edwards, S.J. et al. (2008) Endocrinology 149:1026.
  12. Liao, W.X. et al. (2003) J. Biol. Chem. 278:3713.
  13. Yan, C. et al. (2001) Mol. Endocrinol. 15:854.
Long Name
Bone Morphogenetic Protein 15
Entrez Gene IDs
9210 (Human); 12155 (Mouse); 59302 (Rat)
Alternate Names
BMP15; BMP-15; bone morphogenetic protein 15; GDF-9B; Growth/differentiation factor 9B; ODG2; POF4

Citations for Recombinant Human BMP-15 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Generation of functional oocytes from male mice in vitro
    Authors: K Murakami, N Hamazaki, N Hamada, G Nagamatsu, I Okamoto, H Ohta, Y Nosaka, Y Ishikura, TS Kitajima, Y Semba, Y Kunisaki, F Arai, K Akashi, M Saitou, K Kato, K Hayashi
    Nature, 2023-03-15;615(7954):900-906.
    Species: Mouse
    Sample Types: Transfected Whole Cells
    Applications: Bioassay
  2. Cooperative effects of oocytes and estrogen on the forkhead box L2 expression in mural granulosa cells in mice
    Authors: H Ito, C Emori, M Kobayashi, N Maruyama, W Fujii, K Naito, K Sugiura
    Scientific Reports, 2022-11-23;12(1):20158.
    Species: Mouse
    Sample Types:
    Applications: Bioassay
  3. Mutual Effects of Orexin and Bone Morphogenetic Proteins on Gonadotropin Expression by Mouse Gonadotrope Cells
    Authors: Y Soejima, N Iwata, N Nakayama, S Hirata, Y Nakano, K Yamamoto, A Suyama, K Oguni, T Nada, S Fujisawa, F Otsuka
    International Journal of Molecular Sciences, 2022-08-29;23(17):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Bone morphogenetic protein 15 intrafollicular injection inhibits ovulation in cattle
    Authors: CS Haas, FC Oliveira, MT Rovani, JG Ferst, SF Vargas, AD Vieira, RG Mondadori, LMC Pegoraro, PBD Gonçalves, V Bordignon, R Ferreira, BG Gasperin
    Theriogenology, 2022-02-10;182(0):148-154.
    Species: Bovine
    Sample Types: In Vivo
    Applications: In Vivo
  5. Activation-induced cytidine deaminase is a possible regulator of cross-talk between oocytes and granulosa cells through GDF-9 and SCF feedback system
    Authors: T Iizuka, K Wakae, M Ono, T Suzuki, Y Mizumoto, K Kitamura, SI Horike, M Muramatsu, H Fujiwara
    Scientific Reports, 2021-02-15;11(1):3833.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Cyclooxygenase 2 messenger RNA levels in canine follicular cells: interrelationship with GDF-9, BMP-15, and progesterone
    Authors: M De Los Rey, J Palomino, A Araujo, J Flores, G Ramirez, VH Parraguez, K Aspee
    Domest. Anim. Endocrinol., 2020-08-06;74(0):106529.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Cellular localization and seasonal variation in BMP15 expression in ovary of the catfish Clarias batrachus and its role in ovarian steroidogenesis
    Authors: H Yadav, B Lal
    Theriogenology, 2019-02-14;129(0):14-22.
    Species: Fish - Clarias batrachus (Freshwater Catfish)
    Sample Types: Tissue Explants
    Applications: Bioassay
  8. Effects of MiR-375-BMPR2 as a Key Factor Downstream of BMP15/GDF9 on the Smad1/5/8 and Smad2/3 Signaling Pathways
    Authors: C Liu, B Yuan, H Chen, M Xu, X Sun, J Xu, Y Gao, C Chen, H Jiang, J Zhang
    Cell. Physiol. Biochem., 2018-03-21;46(1):213-225.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  9. In vitro differentiation of human embryonic stem cells into ovarian follicle-like cells
    Authors: D Jung, J Xiong, M Ye, X Qin, L Li, S Cheng, M Luo, J Peng, J Dong, F Tang, W Shen, MM Matzuk, K Kee
    Nat Commun, 2017-06-12;8(0):15680.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Conserved miR-10 family represses proliferation and induces apoptosis in ovarian granulosa cells
    Authors: T Jiajie, Y Yanzhou, AC Hoi-Hung, C Zi-Jiang, C Wai-Yee
    Sci Rep, 2017-01-23;7(0):41304.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Natriuretic peptide type C induces sperm attraction for fertilization in mouse
    Authors: N Kong, X Xu, Y Zhang, Y Wang, X Hao, Y Zhao, J Qiao, G Xia, M Zhang
    Sci Rep, 2017-01-05;7(0):39711.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Cumulin, an Oocyte-secreted Heterodimer of the Transforming Growth Factor-beta Family, Is a Potent Activator of Granulosa Cells and Improves Oocyte Quality.
    Authors: Mottershead D, Sugimura S, Al-Musawi S, Li J, Richani D, White M, Martin G, Trotta A, Ritter L, Shi J, Mueller T, Harrison C, Gilchrist R
    J Biol Chem, 2015-08-08;290(39):24007-20.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  13. Bone morphogenetic protein 15 in the pro-mature complex form enhances bovine oocyte developmental competence.
    Authors: Sudiman J, Sutton-McDowall M, Ritter L, White M, Mottershead D, Thompson J, Gilchrist R
    PLoS ONE, 2014-07-24;9(7):e103563.
    Species: Human
    Sample Types: Protein, Whole Cells
    Applications: Bioassay, Western Blot
  14. BMP15 suppresses progesterone production by down-regulating StAR via ALK3 in human granulosa cells.
    Authors: Chang H, Cheng J, Klausen C, Leung P
    Mol Endocrinol, 2013-10-18;27(12):2093-104.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Genome-wide association studies identify two novel BMP15 mutations responsible for an atypical hyperprolificacy phenotype in sheep.
    Authors: Demars J, Fabre S, Sarry J, Rossetti R, Gilbert H, Persani L, Tosser-Klopp G, Mulsant P, Nowak Z, Drobik W, Martyniuk E, Bodin L
    PLoS Genet, 2013-04-25;9(4):e1003482.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  16. Signalling pathways mediating specific synergistic interactions between GDF9 and BMP15.
    Authors: Mottershead D, Ritter L, Gilchrist R
    Mol Hum Reprod, 2011-09-12;18(3):121-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011-07-07;286(34):30034-46.
    Species: Human, Mouse
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  18. Steady-state level of bone morphogenetic protein-15 in goat ovaries and its influence on in vitro development and survival of preantral follicles.
    Authors: Celestino JJ, Lima-Verde IB, Bruno JB, Matos MH, Chaves RN, Saraiva MV, Silva CM, Faustino LR, Rossetto R, Lopes CA, Donato MA, Peixoto CA, Campello CC, Silva JR, Figueiredo JR
    Mol. Cell. Endocrinol., 2011-02-17;338(1):1-9.
    Species: Goat
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Fibroblast growth factors and epidermal growth factor cooperate with oocyte-derived members of the TGFbeta superfamily to regulate Spry2 mRNA levels in mouse cumulus cells.
    Authors: Sugiura K, Su YQ, Li Q, Wigglesworth K, Matzuk MM, Eppig JJ
    Biol. Reprod., 2009-06-24;81(5):833-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What is the half-life of this BMP protein?

    • R&D Systems has not determined the half-life of this recombinant human BMP protein. Assay conditions for each protein will need to be optimized by each researcher.

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Reviews for Recombinant Human BMP-15 Protein

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Recombinant Human BMP-15 Protein
By Anonymous on 02/18/2021
Application: In vitro bioactivity in cell culture

Recombinant Human BMP-15 Protein
By Pablo Hurtado on 10/09/2020
Application: CellProlif