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Recombinant Human BMP-5

Catalog #: 615-BM
4 Citations Datasheet / COA / SDS
Newer Version Available: 615-BMC
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Discontinued Product

615-BM has been discontinued and is replaced by 615-BMC.

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Citations (4)
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Recombinant Human BMP-5 Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.5-2 µg/mL. Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.5‑1.0 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human BMP-5 protein
Human BMP-2 propeptide
(Ser249 - Arg282)
Accession # P12643.1		 Human BMP-5
(Asn323 - His454)
Accession # P22003.1
N-terminus C-terminus
N-terminal Sequence
Analysis

Ser249 (BMP-2 propeptide) & Asn323 (mature BMP-5)

Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass

19 kDa (monomer of BMP-5 with extra 34 amino acid residues from BMP-2) & 16 kDa (monomer of mature BMP-5)

SDS-PAGE
20 kDa and 25 kDa, reducing conditions

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615-BM (with carrier)

Product Datasheet
COA

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615-BM/CF (carrier free)

Discontinued Product

615-BM

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: BMP-5

Human BMP-5 is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor  beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human and mouse BMP-5 are 97% identical. BMP-5 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. Biologically active BMP-5 is a disulfide-linked homodimer of the carboxy-terminal 132 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-5 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. One BMP type II and two BMP type I receptors have been identified.

References
  1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
  2. Ebendal, T. et al. (1998) J. Neurosci. Res. 51:139.
  3. Reddi, A.H. (1998) Nature Biotechnology 16:247.
Long Name
Bone Morphogenetic Protein 5
Entrez Gene IDs
653 (Human); 12160 (Mouse)
Alternate Names
BMP5; BMP-5; bone morphogenetic protein 5; MGC34244

Citations for Recombinant Human BMP-5

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. New role of bone morphogenetic protein 7 in brown adipogenesis and energy expenditure.
    Authors: Tseng YH, Kokkotou E, Schulz TJ, Huang TL, Winnay JN, Taniguchi CM, Tran TT, Suzuki R, Espinoza DO, Yamamoto Y, Ahrens MJ, Dudley AT, Norris AW, Kulkarni RN, Kahn CR
    Nature, 2008-08-21;454(7207):1000-4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Targeting of bone morphogenetic protein growth factor complexes to fibrillin.
    Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY
    J. Biol. Chem., 2008-03-13;283(20):13874-88.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay, Surface Plasmon Resonance
  3. Requirements for endoderm and BMP signaling in sensory neurogenesis in zebrafish.
    Authors: Holzschuh J, Wada N, Wada C, Schaffer A, Javidan Y, Tallafuss A, Bally-Cuif L, Schilling TF
    Development, 2005-08-01;132(16):3731-42.
    Species: Xenopus
    Sample Types: In Vivo
    Applications: In Vivo
  4. A new role for BMP5 during limb development acting through the synergic activation of Smad and MAPK pathways.
    Authors: Zuzarte-Luis V, Montero JA, Rodriguez-Leon J, Merino R, Rodriguez-Rey JC, Hurle JM
    Dev. Biol., 2004-08-01;272(1):39-52.
    Species: Chicken
    Sample Types: In Vivo
    Applications: In Vivo

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