Recombinant Human BMP-6 Protein

Carrier Free

Catalog # Availability Size / Price Qty
507-BP-020/CF

With Carrier

Catalog # Availability Size / Price Qty
507-BP-020
Recombinant Human BMP-6 Protein Bioactivity
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Product Details
Citations (66)
FAQs
Reviews (2)

Recombinant Human BMP-6 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.02‑0.15 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human BMP-6 protein
Gln382-His513
Accession #
N-terminal Sequence
Analysis
Gln382
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
15 kDa (monomer)
SDS-PAGE
18 kDa, reducing conditions

Product Datasheets

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507-BP (with carrier)

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507-BP/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

507-BP

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

507-BP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl. 
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human BMP-6 Protein Bioactivity View Larger

Recombinant Human BMP-6 (Catalog # 507-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.02‑0.15 μg/mL.

SDS-PAGE Recombinant Human BMP-6 Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human BMP-6 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 18 kDa and 32 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-6

Bone Morphogenetic Protein 6 (BMP-6), also known as Vgr-1, is a member of the BMP subfamily of TGF-beta superfamily proteins. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis (1). Human BMP-6 is synthesized as a 513 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 18 kDa C-terminal mature protein. Biologically active BMP-6 consists of a disulfide-linked homodimer of the mature protein, although it can also form heterodimers with mature BMP-2 (2, 3). Mature human BMP-6 shares 96% and 98% aa sequence identity with mouse and rat BMP-6, respectively. Cellular responses to BMP-6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (4, 5). BMP-6 induces the expression of Noggin and is subsequently antagonized by Noggin (6). BMP-6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (7), chondrocyte maturation (8), Ang II-induced aldosterone production in the adrenal cortex (4), hormone production and responsiveness in ovarian granulosa cells (9), iNOS and TNF-alpha production in macrophages (5), the cell death of B cells (10), and neurite outgrowth (11). BMP-6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). It enhances tumor progression by promoting local angiogenesis and differentiation of immune tolerizing M2 macrophages (13-15). Through interactions with the BMP coreceptor RGM-C/Hemojuvelin, BMP-6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (16). Heterodimers of BMP-2 and BMP-6 show increased potency at inducing osteoblastic calcium deposition, chondrogenesis, and in vivo bone formation compared to either BMP-2 or BMP-6 homodimers (3).

References
  1. Bragdon, B. et al. (2010) Cell Signal. 23:609.
  2. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9843.
  3. Israel, D.I. et al. (1996) Growth Factors 13:291.
  4. Inagaki, K. et al. (2006) Endocrinology 147:2681.
  5. Hong, J.H. et al. (2008) Immunology 128:e442.
  6. Haudenschild, D.R. et al. (2004) Cancer Res. 64:8276.
  7. Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
  8. Grimsrud, C.D. et al. (1999) J. Bone Miner. Res. 14:475.
  9. Shi, J. et al. (2009) Fertil. Steril. 92:1794.
  10. Kersten, C. et al. (2005) BMC Immunol. 6:9.
  11. Yabe, T. et al. (2002) J. Neurosci. Res. 68:161.
  12. Zhang, Z. et al. (2006) Neuroscience 138:47.
  13. Dai, J. et al. (2005) Cancer Res. 65:8274.
  14. Kwon, S.J. et al. (2014) Prostate 74:121.
  15. Lee, J.-H. et al. (2013) Cancer Res. 73:3604.
  16. Andriopoulos, B. Jr. et al. (2009) Nat. Genet. 41:482.
Long Name
Bone Morphogenetic Protein 6
Entrez Gene IDs
654 (Human); 12161 (Mouse); 25644 (Rat)
Alternate Names
BMP6; BMP-6; bone morphogenetic protein 6; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine; VG-1-R; VG-1-related protein; Vg1-related sequence; VGR; VGR1; Vgr-1

Citations for Recombinant Human BMP-6 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

66 Citations: Showing 1 - 10
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  1. Mutual Effects of Orexin and Bone Morphogenetic Proteins on Gonadotropin Expression by Mouse Gonadotrope Cells
    Authors: Y Soejima, N Iwata, N Nakayama, S Hirata, Y Nakano, K Yamamoto, A Suyama, K Oguni, T Nada, S Fujisawa, F Otsuka
    International Journal of Molecular Sciences, 2022;23(17):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Aberrant astrocyte protein secretion contributes to altered neuronal development in multiple models of neurodevelopmental disorders
    Authors: ALM Caldwell, L Sancho, J Deng, A Bosworth, A Miglietta, JK Diedrich, MN Shokhirev, NJ Allen
    Nature Neuroscience, 2022;25(9):1163-1178.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. MDS cells impair osteolineage differentiation of MSCs via extracellular vesicles to suppress normal hematopoiesis
    Authors: Y Hayashi, KC Kawabata, Y Tanaka, Y Uehara, Y Mabuchi, K Murakami, A Nishiyama, S Kiryu, Y Yoshioka, Y Ota, T Sugiyama, K Mikami, M Tamura, T Fukushima, S Asada, R Takeda, Y Kunisaki, T Fukuyama, K Yokoyama, T Uchida, M Hagihara, N Ohno, K Usuki, A Tojo, Y Katayama, S Goyama, F Arai, T Tamura, T Nagasawa, T Ochiya, D Inoue, T Kitamura
    Cell Reports, 2022;39(6):110805.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Iron overload inhibits BMP/SMAD and IL-6/STAT3 signaling to hepcidin in cultured hepatocytes
    Authors: E Charlebois, K Pantopoulo
    PLoS ONE, 2021;16(6):e0253475.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. Functionally diverse heteromeric traps for ligands of the transforming growth factor-beta superfamily
    Authors: R Kumar, AV Grinberg, H Li, TH Kuo, D Sako, L Krishnan, K Liharska, J Li, R Grenha, MC Maguire, SD Briscoe, RS Pearsall, BR Herrin, RNVS Suragani, R Castonguay
    Scientific Reports, 2021;11(1):18341.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  6. Repopulation of decellularised articular cartilage by laser-based matrix engraving
    Authors: S Nürnberger, C Schneider, C Keibl, B Schädl, P Heimel, X Monforte, AH Teuschl, M Nalbach, PJ Thurner, J Grillari, H Redl, S Wolbank
    EBioMedicine, 2021;0(0):103196.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Systematic investigation of cytokine signaling activity at the tissue and single-cell levels
    Authors: P Jiang, Y Zhang, B Ru, Y Yang, T Vu, R Paul, A Mirza, G Altan-Bonn, L Liu, E Ruppin, L Wakefield, KW Wucherpfen
    Nature methods, 2021;18(10):1181-1191.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Comparative Proteomic Analysis of the Mesenchymal Stem Cells Secretome from Adipose, Bone Marrow, Placenta and Wharton's Jelly
    Authors: S Shin, J Lee, Y Kwon, KS Park, JH Jeong, SJ Choi, SI Bang, JW Chang, C Lee
    International Journal of Molecular Sciences, 2021;22(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Biphasic Roles of Clock Genes and Bone Morphogenetic Proteins in Gonadotropin Expression by Mouse Gonadotrope Cells
    Authors: Y Soejima, N Iwata, Y Nakano, K Yamamoto, A Suyama, T Nada, F Otsuka
    International Journal of Molecular Sciences, 2021;22(20):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  10. Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration
    Authors: A Krouwels, JD Iljas, AHM Kragten, WJA Dhert, FC Öner, MA Tryfonidou, LB Creemers
    Int J Mol Sci, 2020;21(8):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  11. A Comparison of Immune Responses Exerted Following Syngeneic, Allogeneic, and Xenogeneic Transplantation of Mesenchymal Stem Cells into the Mouse Brain
    Authors: JW Hwang, NK Lee, JH Yang, HJ Son, SI Bang, JW Chang, DL Na
    Int J Mol Sci, 2020;21(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  12. Gnpat does not play an essential role in systemic iron homeostasis in murine model
    Authors: P An, J Wang, H Wang, L Jiang, J Wang, J Min, F Wang
    J. Cell. Mol. Med., 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  13. Inhibition of bone morphogenetic protein 6 receptors ameliorates Sj�gren's syndrome in mice
    Authors: H Yin, L Kalra, Z Lai, MC Guimaro, L Aber, BM Warner, D Michael, N Zhang, J Cabrera-Pe, A Karim, WD Swaim, S Afione, A Voigt, CQ Nguyen, PB Yu, DB Bloch, JA Chiorini
    Sci Rep, 2020;10(1):2967.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Endothelial cell clonal expansion in the development of cerebral cavernous malformations
    Authors: M Malinverno, C Maderna, A Abu Taha, M Corada, F Orsenigo, M Valentino, F Pisati, C Fusco, P Graziano, M Giannotta, QC Yu, YA Zeng, MG Lampugnani, PU Magnusson, E Dejana
    Nat Commun, 2019;10(1):2761.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. The deubiquitinating enzyme PSMD14 facilitates tumor growth and chemoresistance through stabilizing the ALK2 receptor in the initiation of BMP6 signaling pathway
    Authors: D Seo, SM Jung, JS Park, J Lee, J Ha, M Kim, SH Park
    EBioMedicine, 2019;49(0):55-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  16. Transcriptomic profiling of the myeloma bone-lining niche reveals BMP signalling inhibition to improve bone disease
    Authors: S Gooding, SWZ Olechnowic, EV Morris, AE Armitage, J Arezes, J Frost, E Repapi, JR Edwards, N Ashley, C Waugh, N Gray, E Martinez-H, PJ Lim, SR Pasricha, H Knowles, AJ Mead, K Ramasamy, H Drakesmith, CM Edwards
    Nat Commun, 2019;10(1):4533.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Cancer-associated fibroblast-derived Gremlin 1 promotes breast cancer progression
    Authors: J Ren, M Smid, J Iaria, DCF Salvatori, H van Dam, HJ Zhu, JWM Martens, P Ten Dijke
    Breast Cancer Res., 2019;21(1):109.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. A Targeted Mass Spectrometric Assay for Reliable Sensitive Hepcidin Quantification
    Authors: A Moghieb, L Tesfay, S Nie, M Gritsenko, TL Fillmore, JM Jacobs, RD Smith, FM Torti, SV Torti, T Shi, C Ansong
    Sci Rep, 2019;9(1):7264.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Adipose-tissue-derived therapeutic cells in their natural environment as an autologous cell therapy strategy: the microtissue-stromal vascular fraction
    Authors: S Nürnberger, C Lindner, J Maier, K Strohmeier, C Wurzer, P Slezak, S Suessner, W Holnthoner, H Redl, S Wolbank, E Priglinger
    Eur Cell Mater, 2019;37(0):113-133.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Cerebrospinal fluid from Alzheimer's disease patients as an optimal formulation for therapeutic application of mesenchymal stem cells in Alzheimer's disease
    Authors: J Lee, SJ Kwon, JH Kim, H Jang, NK Lee, JW Hwang, JH Kim, JW Chang, DL Na
    Sci Rep, 2019;9(1):564.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Anterior cruciate ligament-derived mesenchymal stromal cells have a propensity to differentiate into the ligament lineage
    Authors: Y Ogata, Y Mabuchi, K Shinoda, Y Horiike, M Mizuno, K Otabe, EG Suto, N Suzuki, I Sekiya, C Akazawa
    Regen Ther, 2018;8(0):20-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. SMOC2 inhibits calcification of osteoprogenitor and endothelial cells
    Authors: T Peeters, S Monteagudo, P Tylzanowsk, FP Luyten, R Lories, F Cailotto
    PLoS ONE, 2018;13(6):e0198104.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Different characteristics of mesenchymal stem cells isolated from different layers of full term placenta
    Authors: YS Choi, YB Park, CW Ha, JA Kim, JC Heo, WJ Han, SY Oh, SJ Choi
    PLoS ONE, 2017;12(2):e0172642.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Thrombospondin-1 (TSP-1), a new bone morphogenetic protein-2 and -4 (BMP-2/4) antagonist identified in pituitary cells
    Authors: C Sallon, I Callebaut, I Boulay, J Fontaine, D Logeart-Av, C Henriquet, M Pugnière, X Cayla, P Monget, G Harichaux, V Labas, S Canepa, C Taragnat
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Adipose Stromal Cells from Visceral and Subcutaneous Fat Facilitate Migration of Ovarian Cancer Cells via IL-6/JAK2/STAT3 Pathway
    Authors: B Kim, HS Kim, S Kim, G Haegeman, BK Tsang, DN Dhanasekar, YS Song
    Cancer Res Treat, 2017;49(2):338-349.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-? receptor type I ALK5
    Authors: LK Bollum, K Huse, MP Oksvold, B Bai, VI Hilden, L Forfang, SO Yoon, S Wälchli, EB Smeland, JH Myklebust
    PLoS ONE, 2017;12(5):e0177188.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Bone morphogenetic protein (BMP) 9 and BMP10 enhance tumor necrosis factor-?-induced monocyte recruitment to the vascular endothelium mainly via activin receptor-like kinase 2
    Authors: CG Mitrofan, SL Appleby, GB Nash, Z Mallat, ER Chilvers, PD Upton, NW Morrell
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Hepcidin is regulated by promoter-associated histone acetylation and HDAC3
    Authors: SR Pasricha, PJ Lim, TL Duarte, C Casu, D Oosterhuis, K Mleczko-Sa, M Suciu, AR Da Silva, K Al-Hourani, J Arezes, K McHugh, S Gooding, JN Frost, K Wray, A Santos, G Porto, E Repapi, N Gray, SJ Draper, N Ashley, E Soilleux, P Olinga, MU Muckenthal, JR Hughes, S Rivella, TA Milne, AE Armitage, H Drakesmith
    Nat Commun, 2017;8(1):403.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Supraphysiological levels of GDF11 induce striated muscle atrophy
    Authors: DW Hammers, M Merscham-B, JY Hsiao, S Engst, JJ Hartman, HL Sweeney
    EMBO Mol Med, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. BMP-9 interferes with liver regeneration and promotes liver fibrosis
    Authors: K Breitkopf-, C Meyer, C König, H Gaitantzi, A Addante, M Thomas, E Wiercinska, C Cai, Q Li, F Wan, C Hellerbran, NA Valous, M Hahnel, C Ehlting, JG Bode, S Müller-Boh, U Klingmülle, J Altenöder, I Ilkavets, MJ Goumans, LJ Hawinkels, SJ Lee, M Wieland, C Mogler, MP Ebert, B Herrera, H Augustin, A Sánchez, S Dooley, P Ten Dijke
    Gut, 2017;0(0):.
    Species: Mouse
    Sample Types:
  31. Anatomically shaped tissue-engineered cartilage with tunable and inducible anticytokine delivery for biological joint resurfacing
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Tumor-Derived Factors and Reduced p53 Promote Endothelial Cell Centrosome Over-Duplication
    PLoS ONE, 2016;11(12):e0168334.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Aquaporin gene therapy corrects Sj�gren's syndrome phenotype in mice
    Proc Natl Acad Sci USA, 2016;113(20):5694-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Effects of Anti-repulsive Guidance Molecule C (RGMc/Hemojuvelin) Antibody on Hepcidin and Iron in Mouse Liver and Tumor Xenografts
    Authors: SV Torti, E Lemler, BK Mueller, A Popp, FM Torti
    Clin Exp Pharmacol, 2016;6(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Bone morphogenetic proteins are mediators of luteolysis in the human corpus luteum.
    Authors: Nio-Kobayashi J, Trendell J, Giakoumelou S, Boswell L, Nicol L, Kudo M, Sakuragi N, Iwanaga T, Duncan W
    Endocrinology, 2015;156(4):1494-503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Hepcidin regulation in prostate and its disruption in prostate cancer.
    Authors: Tesfay L, Clausen K, Kim J, Hegde P, Wang X, Miller L, Deng Z, Blanchette N, Arvedson T, Miranti C, Babitt J, Lin H, Peehl D, Torti F, Torti S
    Cancer Res, 2015;75(11):2254-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Analysis of the effects of five factors relevant to in vitro chondrogenesis of human mesenchymal stem cells using factorial design and high throughput mRNA-profiling.
    Authors: Jakobsen R, Ostrup E, Zhang X, Mikkelsen T, Brinchmann J
    PLoS ONE, 2014;9(5):e96615.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Bone morphogenetic protein-9 suppresses growth of myeloma cells by signaling through ALK2 but is inhibited by endoglin.
    Authors: Olsen O, Wader K, Misund K, Vatsveen T, Ro T, Mylin A, Turesson I, Stordal B, Moen S, Standal T, Waage A, Sundan A, Holien T
    Blood Cancer J, 2014;4(0):e196.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  39. Chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold.
    Authors: Ye K, Felimban R, Traianedes K, Moulton S, Wallace G, Chung J, Quigley A, Choong P, Myers D
    PLoS ONE, 2014;9(6):e99410.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis.
    Authors: Brady K, Dickinson S, Guillot P, Polak J, Blom A, Kafienah W, Hollander A
    Stem Cells Dev, 2014;23(5):541-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Unbiased RNAi screen for hepcidin regulators links hepcidin suppression to proliferative Ras/RAF and nutrient-dependent mTOR signaling.
    Authors: Mleczko-Sanecka K, Roche F, Da Silva A, Call D, D'Alessio F, Ragab A, Lapinski P, Ummanni R, Korf U, Oakes C, Damm G, D'Alessandro L, Klingmuller U, King P, Boutros M, Hentze M, Muckenthaler M
    Blood, 2014;123(10):1574-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  42. MicroRNA-130a is up-regulated in mouse liver by iron deficiency and targets the bone morphogenetic protein (BMP) receptor ALK2 to attenuate BMP signaling and hepcidin transcription.
    Authors: Zumbrennen-Bullough K, Wu Q, Core A, Canali S, Chen W, Theurl I, Meynard D, Babitt J
    J Biol Chem, 2014;289(34):23796-808.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. Reduced BMP6 expression by DNA methylation contributes to EMT and drug resistance in breast cancer cells.
    Authors: Liu G, Liu Y, Lian W, Zhao Z, Yi T, Zhou H
    Oncol Rep, 2014;32(2):581-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Brown fat determination and development from muscle precursor cells by novel action of bone morphogenetic protein 6.
    Authors: Sharma A, Huard C, Vernochet C, Ziemek D, Knowlton K, Tyminski E, Paradis T, Zhang Y, Jones J, von Schack D, Brown C, Milos P, Coyle A, Tremblay F, Martinez R
    PLoS ONE, 2014;9(3):e92608.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  45. Functional link between bone morphogenetic proteins and insulin-like peptide 3 signaling in modulating ovarian androgen production.
    Authors: Glister C, Satchell L, Bathgate R, Wade J, Dai Y, Ivell R, Anand-Ivell R, Rodgers R, Knight P
    Proc Natl Acad Sci U S A, 2013;110(15):E1426-35.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  46. MicroRNA-22 is a master regulator of bone morphogenetic protein-7/6 homeostasis in the kidney.
    Authors: Long, Jianyin, Badal, Shawn S, Wang, Yin, Chang, Benny H, Rodriguez, Antony, Danesh, Farhad R
    J Biol Chem, 2013;288(51):36202-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  47. Repulsive guidance molecule (RGM) family proteins exhibit differential binding kinetics for bone morphogenetic proteins (BMPs).
    Authors: Wu, Qifang, Sun, Chia Chi, Lin, Herbert, Babitt, Jodie L
    PLoS ONE, 2012;7(9):e46307.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  48. Derivation of cerebellar neurons from human pluripotent stem cells.
    Authors: Erceg S, Lukovic D, Moreno-Manzano V, Stojkovic M, Bhattacharya S
    Curr Protoc Stem Cell Biol, 2012;20(0):1H.51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Signalling pathways mediating specific synergistic interactions between GDF9 and BMP15.
    Authors: Mottershead D, Ritter L, Gilchrist R
    Mol Hum Reprod, 2012;18(3):121-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  50. Role of Smad proteins in resistance to BMP-induced growth inhibition in B-cell lymphoma.
    Authors: Huse K, Bakkebo M, Walchli S, Oksvold M, Hilden V, Forfang L, Bredahl M, Liestol K, Alizadeh A, Smeland E, Myklebust J
    PLoS ONE, 2012;7(10):e46117.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  51. Bone morphogenetic proteins inhibit CD40L/IL-21-induced Ig production in human B cells: differential effects of BMP-6 and BMP-7.
    Authors: Huse K, Bakkebo M, Oksvold MP, Forfang L, Hilden VI, Stokke T, Smeland EB, Myklebust JH
    Eur. J. Immunol., 2011;41(11):3135-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  52. Regulation of TMPRSS6 by BMP6 and iron in human cells and mice.
    Authors: Meynard D, Vaja V, Sun CC, Corradini E, Chen S, Lopez-Otin C, Grgurevic L, Hong CC, Stirnberg M, Gutschow M, Vukicevic S, Babitt JL, Lin HY
    Blood, 2011;118(3):747-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  53. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011;286(34):30034-46.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  54. Bone morphogenetic proteins and receptors are over-expressed in bone-marrow cells of multiple myeloma patients and support myeloma cells by inducing ID genes.
    Authors: Grcevic D, Kusec R, Kovacic N, Lukic A, Lukic IK, Ivcevic S, Nemet D, Seiwerth RS, Ostojic SK, Croucher PI, Marusic A
    Leuk. Res., 2010;34(6):742-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  55. The 12th-14th type III repeats of fibronectin function as a highly promiscuous growth factor-binding domain.
    Authors: Martino MM, Hubbell JA
    FASEB J., 2010;24(12):4711-21.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  56. Fibroblast growth factors and epidermal growth factor cooperate with oocyte-derived members of the TGFbeta superfamily to regulate Spry2 mRNA levels in mouse cumulus cells.
    Authors: Sugiura K, Su YQ, Li Q, Wigglesworth K, Matzuk MM, Eppig JJ
    Biol. Reprod., 2009;81(5):833-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  57. A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
    Authors: Herrera B, Inman GJ,
    BMC Cell Biol., 2009;10(0):20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  58. Effect of direct ovarian infusion of bone morphogenetic protein 6 (BMP6) on ovarian function in sheep.
    Authors: Campbell BK, Kendall NR, Baird DT
    Biol. Reprod., 2009;81(5):1016-23.
    Species: Ovine
    Sample Types: In Vivo
    Applications: In Vivo
  59. New role of bone morphogenetic protein 7 in brown adipogenesis and energy expenditure.
    Authors: Tseng YH, Kokkotou E, Schulz TJ, Huang TL, Winnay JN, Taniguchi CM, Tran TT, Suzuki R, Espinoza DO, Yamamoto Y, Ahrens MJ, Dudley AT, Norris AW, Kulkarni RN, Kahn CR
    Nature, 2008;454(7207):1000-4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  60. Targeting of bone morphogenetic protein growth factor complexes to fibrillin.
    Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY
    J. Biol. Chem., 2008;283(20):13874-88.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  61. Sequential roles for myosin-X in BMP6-dependent filopodial extension, migration, and activation of BMP receptors.
    Authors: Pi X, Ren R, Kelley R, Zhang C, Moser M, Bohil AB, Divito M, Cheney RE, Patterson C
    J. Cell Biol., 2007;179(7):1569-82.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  62. A rapid and sensitive bioassay to measure bone morphogenetic protein activity.
    Authors: Zilberberg L, ten Dijke P, Sakai L, Rifkin D
    BMC Cell Biol, 2007;8(0):41.
    Applications: Bioassay
  63. Differentiation of ES cells into cerebellar neurons.
    Authors: Salero E, Hatten ME
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(8):2997-3002.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  64. BMP-6 inhibits human bone marrow B lymphopoiesis--upregulation of Id1 and Id3.
    Authors: Kersten C, Dosen G, Myklebust JH, Sivertsen EA, Hystad ME, Smeland EB, Rian E
    Exp. Hematol., 2006;34(1):72-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  65. hCHL2, a novel chordin-related gene, displays differential expression and complex alternative splicing in human tissues and during myoblast and osteoblast maturation.
    Authors: Oren A, Toporik A, Biton S, Almogy N, Eshel D, Bernstein J, Savitsky K, Rotman G
    Gene, 2004;331(0):17-31.
    Species: Human
    Sample Types:
    Applications: Bioassay
  66. BMPER, a novel endothelial cell precursor-derived protein, antagonizes bone morphogenetic protein signaling and endothelial cell differentiation.
    Authors: Moser M, Binder O, Wu Y, Aitsebaomo J, Ren R, Bode C, Bautch VL, Conlon FL, Patterson C
    Mol. Cell. Biol., 2003;23(16):5664-79.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What is the half-life of a recombinant or natural protein in culture at 37 °C?

    • R&D Systems has not determined the half-life of recombinant or natural proteins in culture. For cell-based bioassays, the incubation period of the protein with the cells typically ranges from 48 to 96 hours. Assay conditions for each protein will need to be optimized by each researcher.

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Reviews for Recombinant Human BMP-6 Protein

Average Rating: 4.5 (Based on 2 Reviews)

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Recombinant Human BMP-6 Protein
By Justin Provencher on 10/09/2019
Application: CellProlif
Reason for Rating: This worked as expected in many experiments. Great product as usual from R&amp;D Systems.

Recombinant Human BMP-6 Protein
By lyndsay wylie on 06/14/2016
Application: In vitro bioactivity in cell culture
Reason for Rating: Works well for inducing BMP signaling