Recombinant Human BMP-7 Biotinylated Protein: R&D Systems

Recombinant Human BMP-7 Biotinylated Protein

BT354 has been discontinued and is replaced by BT354B.
  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 100-600 ng/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived Ser293-His431
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser293
  • Structure / Form
    Disulfide-linked homodimer; Biotinylated protein via sugars
  • Predicted Molecular Mass
    16 kDa (unlabeled)
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
BT354
 
BT354/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Both Biotinylated Recombinant Human BMP-7 (Catalog # BT354) and unlabeled Recombinant Human BMP-7 (Catalog # 354-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 100-600 ng/mL. The similarity in activity highlights that the biotinylated protein is fully functional.
Background: BMP-7
Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), is a widely expressed TGF-beta superfamily member with important functions during embryogenesis, in the adult, and in disease (1, 2). Human BMP-7 is synthesized with a 29 amino acid (aa) signal sequence, a 263 aa propeptide, and a 139 aa growth factor domain (3, 4). The growth factor domain of human BMP-7 shares 98% aa sequence identity with mouse and rat BMP-7. The BMP-7 propeptide is cleaved intracellularly but remains in association with the growth factor domain. BMP-7 is subsequently secreted as a tetramer that consists of two propeptides and two disulfide-linked growth factor domains (5, 6). Mature BMP-7 can also form disulfide-linked heterodimers with BMP-2 or BMP-4, complexes that show increased potency and range of activity compared to BMP-7 homodimers (7-9). The presence of the propeptides in the BMP-7 tetramer does not diminish the bioactivity of the growth factor domains (6). Secreted BMP-7 is immobilized in the extracellular matrix as a result of interactions between the propeptide and matrix Fibrillin (5). BMP-7 exerts its biological effects through the type 2 receptors Activin RIIA, Activin RIIB, and BMPR-II and the type 1 receptors Activin RIA, BMPR-IA, and BMPR-IB (2, 6). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (10, 11), inhibits the branching of prostate epithelium (12), and antagonizes epithelial-mesenchymal transition (EMT) (13-15). In pathological conditions, BMP-7 inhibits tumor growth and metastasis (14), ameliorates fibrotic damage in nephritis (13), and promotes neuroregeneration following brain ischemia (16).
  • References:
    1. Carreira, A.C. et al. (2015) J. Dent. Res. 93:335.
    2. Weiskirchen, R. and S.K. Meurer (2013) Front. Biosci. (Landmark Ed.) 18:1407.
    3. Ozkaynak, E. et al. (1990) EMBO J. 9:2085.
    4. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9843.
    5. Gregory, K.E. et al. (2005) J. Biol. Chem. 280:27970.
    6. Sengle, G. et al. (2008) J. Mol. Biol. 381:1025.
    7. Israel, D.I. et al. (1996) Growth Factors 13:291.
    8. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
    9. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
    10. Sampath, T.K. et al. (1992) J. Biol. Chem. 267:20352.
    11. Kazama, I. et al. (2008) J. Am. Soc. Nephrol. 19:2181.
    12. Grishina, I.B. et al. (2005) Dev. Biol. 288:334.
    13. Zeisberg, M. et al. (2003) Nat. Med. 9:964.
    14. Buijs, J.T. et al. (2007) Am. J. Pathol. 171:1047.
    15. Yu, M.-A. et al. (2009) J. Am. Soc. Nephrol. 20:567.
    16. Chou, J. et al. (2006) J. Neurol. Sci. 240:21.
  • Long Name:
    Bone Morphogenetic Protein 7
  • Entrez Gene IDs:
    655 (Human); 12162 (Mouse); 85272 (Rat)
  • Alternate Names:
    BMP7; BMP-7; bone morphogenetic protein 7; Eptotermin alfa; OP-1; OP-1OP1; Osteogenic protein 1
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    BT354 has been replaced by
    BT354B

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