Recombinant Human Cadherin-7 (CAD-7) Protein, CF

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Recombinant Human Cadherin-7 (CAD-7) Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of U‑87 MG human glioblastoma/astrocytoma cells.

The ED50 for this effect is 1.0-4.0 μg/mL.

Optimal dilutions should be determined by each laboratory for each application.

Chinese Hamster Ovary cell line, CHO-derived human Cadherin-7 protein
Met1-Thr607, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
62.2 kDa
80 - 90 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: Cadherin-7

Cadherin-7 is an approximately 115 kDa type I transmembrane protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1). Human Cadherin-7 is synthesized with a 27 amino acid (aa) signal peptide and a 20 aa N-terminal propeptide. The mature cell surface-expressed protein consists of a 738 amino acid (aa) extracellular domain (ECD) that contains five Cadherin repeats, a 21 aa transmembrane segment, and a 157 aa cytoplasmic domain (2, 3). Within the ECD, human Cadherin-7 shares 99% and 97% aa sequence identity with mouse and rat Cadherin-7, respectively. Cadherin-7 interacts homotypically and heterotypically with Cadherin-14 and more weakly with Cadherins-6, -9, and -12 (3, 4). Cellular adhesion mediated by Cadherin-7 is more weak than that mediated by E- or N-Cadherin, although it can be strengthed by Fibronectin binding to Integrins on the same cell (5, 6). Cadherin-7 is localized to discrete regions of the developing nervous system. In chick and mouse, it is expressed in the basal plate of the neural tube, migrating cranial motoneurons, and migrating neural crest cells (4, 7, 8), lateral regions of the hindbrain and migrating Purkinje cell precursors (8, 9), striatum, parahippocampal areas, and somatosensory cortex of the forebrain (10 - 12), neural retina and cochlea (13, 14). Cadherin-7 interactions promote motor axon growth and inhibit axonal branching, whereas Cadherin-6B promotes branching during cranial motorneuron development (7). Cadherin-7 performs a similar function during limb bud development during which it participates in the migration and condensation of mesenchymal cells (15). Cadherin-7 is overexpressed on primary melanoma cells where it binds melanoma inhibitory activity (MIA), a secreted melanoma cell protein that promotes tumor progression (16).

  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Kools, P. et al. (2000) Genomics 68:283.
  3. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
  4. Nakagawa, S. et al. (1995) Development 121:1321.
  5. Chu, Y.-S. et al. (2006) J. Biol. Chem. 281:2901.
  6. Martinez-Rico, C. et al. (2009) J. Cell Sci. 123:712.
  7. Barnes, S.H. et al. (2010) Development 137:805.
  8. Ju, M.J. et al. (2004) Neuroscience 128:785.
  9. Luo, J. et al. (2004) Mol. Cell. Neurosci. 25:138.
  10. Redies, C. et al. (2002) Brain Res. Bull. 57:489.
  11. Kovjanic, D. and C. Redies (2003) J. Comp. Neurol. 456:95.
  12. Krishna-K, K. et al. (2011) Neuroscience 175:37.
  13. Faulkner-Jones, B.E. et al. (1999) Mol. Cell. Neurosci. 14:1.
  14. Luo, J. et al. (2007) Dev. Dyn. 236:2331.
  15. Kim, D. et al. (2009) J. Cell. Physiol. 221:161.
  16. Winklmeier, A. et al. (2009) Cancer Sci. 100:261.
Entrez Gene IDs
1005 (Human); 241201 (Mouse); 29162 (Rat)
Alternate Names
CAD7; cadherin 7, type 2; Cadherin7; Cadherin-7; CDH7; CDH7L1


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