Recombinant Human Calsyntenin-1 Protein, CF
Recombinant Human Calsyntenin-1 Protein, CF Summary
Optimal dilutions should be determined by each laboratory for each application.
Arg30-Thr851, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 300 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Calsyntenin-1 (CST-1; gene name CLSTN1), also called Alcadein-alpha, is a 140‑150 kDa type I transmembrane glycoprotein that is member of the Alcadein family of cadherin domain-containing molecules (1, 2). The human Calsyntenin-1 cDNA encodes 981 amino acids (aa), including a 28 aa signal sequence and the 953 aa mature protein (1). Further cleavage by ADAM10 and ADAM17 generates an N-terminal 115 kDa portion containing the two cadherin domains (aa 38‑265), termed soluble Alc‑ alpha, and a C-terminal portion, termed CTF1-alpha (1, 3). CTF1-alpha contains 34 extracellular aa, 21 transmembrane aa, and 101 cytoplasmic aa that are highly acidic and bind calcium (1, 4). Isoforms of 962-994 aa contain a 78 aa substitution for aa 917‑981, with or without deletions of aa 72‑81 and 507‑525. Within the soluble Alc‑ alpha portion (aa 30‑851), human Calsyntenin-1 shares 92% aa sequence identity with mouse, 93% with equine, and 91% with rat, canine, bovine and porcine Calsyntentin-1. It is expressed in neuronal post-synaptic plasma membranes, neuronal APP-containing early endosomal vesicles (full-length CST-1) and APP-negative recycling‑endosomal vesicles (CST-1 cleaved form), plus the secretory granules of anterior pituitary basophilic gonadotrophs and pancreatic islet alpha -cells (1-6). Soluble Alc‑ alpha mediates adhesion and can be released from axons, while CTF1-alpha can be internalized and mediates trafficking of vesicles via interaction with Kinesin-1 (1, 6-9). This interaction facilitates intracellular transport of vesicles along microtubules in axonal growth cones (7-10). Calsyntenin regulates amyloid precursor protein (APP) cleavage and transport, and perturbation of axonal transport of APP by CST-1 containing carriers results in increased production of A beta. Finally, CST-1 may also play a regulatory role in post-synaptic signaling (1, 2, 4, 8, 10).
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- Steuble, M. et al. (2010) Proteomics 10:3775.
- Konecna, A. et al. (2006) Mol. Biol. Cell 17:3651.
- Araki, Y. et al. (2007) EMBO J. 26:1475.
- Vagnoni, A. et al. (2011) J. Cell Sci. 24:1032.
- Ludwig, A. et al. (2009) Traffic 10:572.
- Vagnoni, A. et al. (2012) Hum. Mol. Genet. Mar. 27 [Epub ahead of print].
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