Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II‑associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1‑16), a pro region (residues 17‑114), and the mature enzyme (residues 115‑331) (3‑5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages and other phagocytic cells.
Recombinant Human Cathepsin S Protein, CF
R&D Systems | Catalog # 1183-CY
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Key Product Details
- R&D Systems NS0-derived Recombinant Human Cathepsin S Protein (1183-CY)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Pro and mature forms
Applications
Enzyme Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Cathepsin S protein
Gln17-Ile331 (pro) & Ser109-Ile331 (mature), both with a C-terminal 10-His tag
Gln17-Ile331 (pro) & Ser109-Ile331 (mature), both with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gln17 predicted & Ser109
Predicted Molecular Mass
39 kDa (Pro) and 28 kDa (mature)
SDS-PAGE
37-41 kDa and 26-27 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >300 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 5 using 1183-CY in the following applications:
Formulation, Preparation, and Storage
1183-CY
| Formulation | Supplied as a 0.2 μm filtered solution in MES, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cathepsin S
References
- Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104 - 1107, Academic Press, San Diego.
- Turk, V. et al. (2001) EMBO J. 20:4629.
- Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
- Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
- Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.
Alternate Names
CTSS
Entrez Gene IDs
1520 (Human)
Gene Symbol
CTSS
UniProt
Additional Cathepsin S Products
Product Documents for Recombinant Human Cathepsin S Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Cathepsin S Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human Cathepsin S Protein, CF
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Protocols
View specific protocols for Recombinant Human Cathepsin S Protein, CF (1183-CY):
Materials
- Assay Buffer: 50 mM NaOAc, 5 mM DTT, 250 mM NaCl, pH 4.5
- Recombinant Human Cathepsin S (rhCathepsin S) (Catalog # 1183-CY)
- Substrate: MCA-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2 (Catalog # ES002)
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rhCathepsin S to 10 µg/mL in Assay Buffer.
- Incubate at room temperature for 2 hours.
- Dilute rhCathepsin S to 0.5 ng/µL in Assay Buffer.
- Dilute Substrate to 20 µM in Assay Buffer.
- Load 50 µL of the 0.5 ng/µL rhCathepsin S into a black well plate, and start the reaction by adding 50 µL of 20 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 20 µM Substrate without any rhCathepsin S.
- Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975)
Per Well:
- rhCathepsin S: 0.025 µg
- Substrate: 10 µM