Recombinant Human Cbl-B Protein, CF

Catalog # Availability Size / Price Qty
E3-306-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Cbl-B Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Typical concentration to support in vitro applications will depend on experimental design.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Cbl-B protein
Accession #
Predicted Molecular Mass
110 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-306

Formulation X mg/ml (X  μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 48 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: Cbl-B

Cbl-B (Casitas B-lineage lymphoma protooncogene b, aka RNF56) is a member of the Cbl family of RING-type E3 Ubiquitin ligases, and has been implicated in the regulation of pathways associated with cell differentiation, tissue development, skeletal muscle atrophy and adaptive immunity.  Along with its RING-type zinc finger domain, Cbl-B contains an N-terminal tyrosine kinase binding (TKB) domain and a C-terminal UBA (Ubiquitin Associated) domain.  This E3 ligase preferentially interacts with phosphotyrosine-modifed intracellular signaling molecules, targeting them for ubiquitination.  Reported substrates for Cbl-B include VAV1, PIKR1, SYK, SRC, EGFR, AXL and others.  Downregulation of Cbl-B has been shown to remove the requirement for CD28 co-stimulation during T-cell activation, suggesting Cbl-B inhibitors may be useful in various cancer immunotherapies.

References
  1. Kobashigawa Y. et al. (2011) Proc. Nat. Acad. Sci08: 20579
  2. Rorsman C. et al. (2016) J. Biol. Chem.  291: 11608
  3. Sitaram P. et al. (2019) Int. J. Mol. Sci.  20: 5821
Long Name
Cbl Proto-Oncogene B
Entrez Gene IDs
868 (Human)
Alternate Names
Cas-Br-M (murine) ecotropic retroviral transforming sequence b; Cas-Br-M (murine) ectropic retroviral transforming sequence b; Casitas B-lineage lymphoma proto-oncogene b; CBLB; Cbl-B; DKFZp686J10223; DKFZp779A0729; DKFZp779F1443; E3 ubiquitin-protein ligase CBL-B; EC 6.3.2; EC 6.3.2.-; FLJ41152; Nbla00127; RING finger protein 56; RNF56; RNF56FLJ36865; SH3-binding protein CBL-B; Signal transduction protein CBL-B

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