Recombinant Human Cbl-B Protein, CF

R&D Systems | Catalog # E3-306

Will be discontinued when existing inventory is gone.
R&D Systems

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

Q13191.2

Applications

Bioactivity
View all Cbl-B Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Cbl-B protein
Met1 - Leu982

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

110 kDa

Activity

Typical concentration to support in vitro applications will depend on experimental design.

Formulation, Preparation, and Storage

E3-306
Formulation Supplied as a solution in HEPES, NaCl, Glycerol and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Cbl-B

Cbl-B (Casitas B-lineage lymphoma protooncogene b, aka RNF56) is a member of the Cbl family of RING-type E3 Ubiquitin ligases, and has been implicated in the regulation of pathways associated with cell differentiation, tissue development, skeletal muscle atrophy and adaptive immunity.  Along with its RING-type zinc finger domain, Cbl-B contains an N-terminal tyrosine kinase binding (TKB) domain and a C-terminal UBA (Ubiquitin Associated) domain.  This E3 ligase preferentially interacts with phosphotyrosine-modifed intracellular signaling molecules, targeting them for ubiquitination.  Reported substrates for Cbl-B include VAV1, PIKR1, SYK, SRC, EGFR, AXL and others.  Downregulation of Cbl-B has been shown to remove the requirement for CD28 co-stimulation during T-cell activation, suggesting Cbl-B inhibitors may be useful in various cancer immunotherapies.

References

  1. Kobashigawa, Y. et al. (2011) Proc. Nat. Acad. Sci. 108: 20579.
  2. Rorsman, C. et al. (2016) J. Biol. Chem. 291: 11608.
  3. Sitaram, P. et al. (2019) Int. J. Mol. Sci. 20: 5821.

Long Name

Cbl Proto-Oncogene B

Alternate Names

CBLB, Nbla00127, RNF56

Entrez Gene IDs

868 (Human)

Gene Symbol

CBLB

UniProt

Q13191.2

Additional Cbl-B Products

Product Documents for Recombinant Human Cbl-B Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Cbl-B Protein, CF

For research use only

Related Research Areas

E3 Ubiquitin Ligases

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