Recombinant Human CD14 Protein

Formulations:
Catalog # Availability Size / Price Qty
383-CD-050
Product Details
Citations (17)
FAQs
Reviews (1)

Recombinant Human CD14 Protein Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP‑1 human acute monocytic leukemia cells. The ED50 for this effect is 0.75‑4.5 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human CD14 protein
Thr20-Cys352
Accession #
N-terminal Sequence
Analysis
Thr20
Predicted Molecular Mass
35.8 kDa
SDS-PAGE
50 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

383-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

383-CD/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD14

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

References
  1. Wright, S.D. et al. (1990) Science 249:1431.
  2. Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
  3. Beutler, B. (2000) Current Opinion in Immunology 12:20.
  4. Stelter, F. (2000) Chem. Immunol. 74:25.
Entrez Gene IDs
929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
Alternate Names
CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein

Citations for Recombinant Human CD14 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Tie2 protects the vasculature against thrombus formation in systemic inflammation
    Authors: SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh
    J. Clin. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Estradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4
    Authors: B S Lashkari, DO Anumba
    PLoS ONE, 2017;12(3):e0173646.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Extracellular Vesicle Proteins Associated with Systemic Vascular Events Correlate with Heart Failure: An Observational Study in a Dyspnoea Cohort.
    Authors: Zhang Y, Vernooij F, Ibrahim I, Ooi S, Gijsberts C, Schoneveld A, Sen K, den Ruijter H, Timmers L, Richards A, Jong C, Mazlan I, Wang J, Lam C, de Kleijn D
    PLoS ONE, 2016;11(1):e0148073.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Developmet
  4. CD14 Is a Co-Receptor for TLR4 in the S100A9-Induced Pro-Inflammatory Response in Monocytes
    PLoS ONE, 2016;11(5):e0156377.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  5. Granzymes A and K differentially potentiate LPS-induced cytokine response
    Authors: Annette C Wensink
    Cell Death Discov, 2016;2(0):16084.
    Applications: Bioassay
  6. Recombinant thrombomodulin inhibits lipopolysaccharide-induced inflammatory response by blocking the functions of CD14.
    Authors: Ma C, Chang W, Shi G, Chang B, Cheng S, Shih Y, Wu H
    J Immunol, 2015;194(4):1905-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Proteasome proteolysis supports stimulated platelet function and thrombosis.
    Authors: Gupta N, Li W, Willard B, Silverstein R, McIntyre T
    Arterioscler Thromb Vasc Biol, 2014;34(1):160-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Directed evolution of an LBP/CD14 inhibitory peptide and its anti-endotoxin activity.
    Authors: Fang L, Xu Z, Wang G, Ji F, Mei C, Liu J, Wu G
    PLoS ONE, 2014;9(7):e101406.
    Species: Human
    Sample Types: Virus
    Applications: Enzyme Assay
  9. Outer membrane vesicles derived from Escherichia coli up-regulate expression of endothelial cell adhesion molecules in vitro and in vivo.
    Authors: Kim J, Yoon Y, Lee J, Choi E, Yi N, Park K, Park J, Lotvall J, Kim Y, Gho Y
    PLoS ONE, 2013;8(3):e59276.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Sialyl residues modulate LPS-mediated signaling through the Toll-like receptor 4 complex.
    Authors: Feng C, Stamatos NM, Dragan AI, Medvedev A, Whitford M, Zhang L, Song C, Rallabhandi P, Cole L, Nhu QM, Vogel SN, Geddes CD, Cross AS
    PLoS ONE, 2012;7(4):e32359.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Iris pigment epithelial cells express a functional lipopolysaccharide receptor complex.
    Authors: Chui JJ, Li MW, Di Girolamo N, Chang JH, McCluskey PJ, Wakefield D
    Invest. Ophthalmol. Vis. Sci., 2010;51(5):2558-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. CD14-independent responses induced by a synthetic lipid A mimetic.
    Authors: Legat A, Thomas S, Hermand P, Van Mechelen M, Goldman M, De Wit D
    Eur J Immunol, 2010;40(3):797-802.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells.
    Authors: Zunt SL, Burton LV, Goldblatt LI, Dobbins EE, Srinivasan M
    Clin. Exp. Immunol., 2009;156(2):285-93.
    Species: N/A
    Sample Types: N/A
    Applications: Western Blot
  14. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  15. TLR4-NOX4-AP-1 signaling mediates lipopolysaccharide-induced CXCR6 expression in human aortic smooth muscle cells.
    Authors: Patel DN, Bailey SR, Gresham JK, Schuchman DB, Shelhamer JH, Goldstein BJ, Foxwell BM, Stemerman MB, Maranchie JK, Valente AJ, Mummidi S, Chandrasekar B
    Biochem. Biophys. Res. Commun., 2006;347(4):1113-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Killing the messenger in the nick of time: persistence of breast milk sCD14 in the neonatal gastrointestinal tract.
    Authors: Blais DR, Harrold J, Altosaar I
    Pediatr. Res., 2006;59(3):371-6.
    Species: N/A
    Sample Types: N/A
    Applications: Western Blot
  17. Induction of keratinocyte growth factor 1 Expression by lipopolysaccharide is regulated by CD-14 and toll-like receptors 2 and 4.
    Authors: Putnins EE, Sanaie AR, Wu Q
    Infect. Immun., 2002;70(12):6541-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human CD14 Protein

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Recombinant Human CD14 Protein
By Anonymous on 11/17/2017
Application: Immunoassay Standard

This recombinant protein was labeled and used as the competitor in a competitive ELISA to measure CD14 in human serum samples. The unlabeled protein was also used to build a calibration curve.