Recombinant Human Cerebellin-1 Protein, CF

R&D Systems | Catalog # 6934-CB

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems CHO-derived Recombinant Human Cerebellin-1 Protein (6934-CB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P23435

Applications

Bioactivity
View all Cerebellin-1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Cerebellin-1 protein
Glu22-Leu193, with an N-terminal HA (YPYDVPDYA) tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr

Predicted Molecular Mass

20 kDa (monomer)

SDS-PAGE

30-35 kDa (monomer) & Oligomer, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Cerebellin-1 is coated at 5 μg/mL, biotinylated recombinant rat NRXN-1 beta binds with an apparent KD < 0.5 nM.

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human Cerebellin-1, immobilized at 3-30 μg/mL, is able to significantly enhance neurite outgrowth.

Formulation, Preparation, and Storage

6934-CB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Cerebellin-1

Cerebellin-1 (CBLN1) is a 35 kDa secreted glycoprotein in the Cerebellin family of TNF superfamily molecules (1). Cerebellins contain an N‑terminal collagenous domain and a C-terminal TNF/C1q-like domain. Mature human Cerebellin-1 shares 100% aa sequence identity with mouse and rat Cerebellin-1. It is expressed in the cerebellum, the parafascicular nucleus of the thalamus, and in the adrenal cortex and pancreatic islets (2 - 5). Cerebellin-1 forms noncovalent homotrimers that are disulfide-linked into hexamers (6). It can also form hetero-oligomers with Cerebellins-2, -3, and -4 and is required for the secretion of Cerebellin-3 (7 - 9). Cerebellin-1 is subject to proteolysis which can liberate 15-mer or 16-mer bioactive peptides from the C1q domain or remove the regions necessary for trimer or hexamer formation (6). Formation of the hexamer is required for Cerebellin-1’s ability to bind to synaptic structures (9, 10). It binds to postsynaptic densities through direct interactions with the glutamate receptor subunit GluR delta 2 and to presynaptic membranes through direct interactions with alpha and beta Neurexins that contain the SS4 insert (11 - 14). The trans-synaptic trimolecular complex of GluR delta 2, Cerebellin-1, and Neurexin promotes both presynaptic and postsynaptic development (11, 12). Cerebellin-1 itself is required for synaptic development, maintenance, and function (10, 12 - 15). It can also be internalized by Purkinje cells following secretion by cerebellar granule cells (2, 8, 15). The 16-mer and 15-mer peptides derived from Cerebellin-1 exert multiple endocrine effects including promoting corticosteroid secretion by cortical adrenal cells, inhibiting glucose‑stimulated insulin secretion from pancreatic islets, promoting Neuropeptide Y secretion by the hypothalamus, and decreasing plasma thyroid stimulating hormone (TSH) levels (4, 5, 16).

References

  1. Yuzaki, M. (2011) Curr. Opin. Neurobiol. 21:215.
  2. Wei, P. et al. (2009) Mol. Cell. Neurosci. 41:258.
  3. Kusnoor, S.V. et al. (2010) J. Comp. Neurol. 518:2525.
  4. Rucinski, M. et al. (2009) Int. J. Mol. Med. 23:363.
  5. Strowski, M.Z. et al. (2009) Regul. Pept. 157:19.
  6. Bao, D. et al. (2005) J. Neurochem. 95:618.
  7. Iijima, T. et al. (2007) Eur. J. Neurosci. 25:1049.
  8. Bao, D. et al. (2006) Mol. Cell. Biol. 26:9327.
  9. Matsuda, K. et al. (2009) Eur. J. Neurosci. 29:707.
  10. Ito-Ishida, A. et al. (2008) J. Neurosci. 28:5920.
  11. Matsuda, K. et al. (2010) Science 328:363.
  12. Matsuda, K. and M. Yuzaki (2011) Eur. J. Neurosci. 33:1447.
  13. Uemura, T. et al. (2010) Cell 141:1068.
  14. Joo, J.-Y. et al. (2011) Biochem. Biophys. Res. Commun. 406:627.
  15. Hirai, H. et al. (2005) Nat. Neurosci. 8:1534.
  16. Gardiner, J.V. et al. (2010) Diabetes Obes. Metab. 12:883.

Alternate Names

CBLN1, Cerebellin1, CLN1, Precerebellin

Entrez Gene IDs

869 (Human); 12404 (Mouse); 498922 (Rat)

Gene Symbol

CBLN1

UniProt

P23435

Additional Cerebellin-1 Products

Product Documents for Recombinant Human Cerebellin-1 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Cerebellin-1 Protein, CF

For research use only

Citations for Recombinant Human Cerebellin-1 Protein, CF

Customer Reviews for Recombinant Human Cerebellin-1 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Cerebellin-1 Protein, CF and earn rewards!

Have you used Recombinant Human Cerebellin-1 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes