Recombinant Human CILP-1 Protein, CF

R&D Systems | Catalog # 5504-CP

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human CILP-1 Protein (5504-CP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_003604

Applications

Bioactivity
View all CILP-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CILP-1 protein
Met1-Arg720, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg22

Predicted Molecular Mass

78.8 kDa

SDS-PAGE

85-95 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.

The ED50 for this effect is 0.5-2.0 μg/mL.

Optimal dilutions should be determined by each laboratory for each application.

Also measured by its ability to bind Recombinant Human TGF‑ beta 1 (Catalog # 240-B).

Formulation, Preparation, and Storage

5504-CP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CILP-1

CILP‑1 (cartilage intermediate‑layer protein 1; CILP C1) is a 92 kDa secreted monomeric cartilage glycoprotein that is the N‑terminal portion of a proteolytically cleaved 138 kD precursor (1, 2). The 62 kDa C‑terminal fragment, called CILP C2, is also secreted; while thought to resemble a nucleoside triphosphate pyrophosphohydrolase (NTPPHase), it has no enzyme activity (2 ‑ 4). Mature human CILP‑1 (aa 22 ‑ 720) contains a TSP‑1 domain (aa 149 ‑ 201) and a C2‑type Ig‑like region (aa 309 ‑ 395) and shares 89% and 42% aa sequence identity with mouse CILP‑1 and human CILP‑2, respectively. CILP‑1 is produced by chondrocytes in both hyaline and fibrocartilage, and preferentially found in the inter‑territorial matrix of the interior layers of articular cartilage (1, 2). Its expression increases with age and correlates with osteoarthritis (1, 2, 5 ‑ 7). While CILP‑1 expression is induced by TGF‑ beta 1‑activated SMAD3, the secreted protein binds and inhibits TGF‑ beta 1 activities, such as induction of cartilage matrix genes (4 ‑ 6, 8). A polymorphism producing the aa substitution I395T enhances inhibition of TGF‑ beta 1 and is significantly associated with lumbar disc disease in Japanese populations, but not in Finnish or Chinese populations (4, 9). Additionally, chondrocyte CILP‑1 expression is inhibited by IGF‑1 in vitro, but CILP‑1 protein blocks IGF‑1 activities such as proliferation and down‑regulation of extracellular inorganic pyrophosphate (ePPi) production (3, 5, 6). In a model of aged cartilage where TGF‑ beta 1 is dominant, increased CILP‑1 inhibits both IGF‑1 and TGF‑ beta 1 activities, slowing cartilage repair due to reduced chondrocyte proliferation and matrix production. At the same time, increased production of ePPi promotes crystals that are a factor in osteoarthritis (3).

References

  1. Lorenzo, P. et al. (1998) J. Biol. Chem. 273:23469.
  2. Lorenzo, P. et al. (1998) J. Biol. Chem. 273:23463.
  3. Johnson, K. et al. (2003) Arthritis Rheum. 48:1302.
  4. Seki, S. et al. (2005) Nat. Genet. 37:607.
  5. Hirose, J. et al. (2002) Arthritis Rheum. 46:3218.
  6. Hirose, J. et al. (2000) Arthritis Rheum. 43:2703.
  7. Lorenzo, P. et al. (2004) Matrix Biol. 23:381.
  8. Mori, M. et al. (2006) Biochem. Biophys. Res. Commun. 341:121.
  9. Virtanen, I.M. et al. (2007) J. Med. Genet. 44:285.

Long Name

Cartilage Intermediate Layer Protein 1

Alternate Names

CILP, CILP1

Entrez Gene IDs

8483 (Human); 214425 (Mouse); 315761 (Rat)

Gene Symbol

CILP

UniProt

NP_003604

Additional CILP-1 Products

Product Documents for Recombinant Human CILP-1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human CILP-1 Protein, CF

For research use only

Citations for Recombinant Human CILP-1 Protein, CF

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