Recombinant Human CL-P1/COLEC12 Protein, CF
Recombinant Human CL-P1/COLEC12 Protein, CF Summary
Leu57-Leu742, with an N-terminal 9-His tag
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Collectins are a family of Ca++-dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1 - 4). Human collectin placenta 1 (CL-P1; also known as collectin sub-family member 12 and SRCL type I [scavenger receptor with C-type lectin type I]) is a 110 kDa member of the collectin family of glycoproteins (5, 6). With two exceptions, all collectins are secreted. CL-P1 is the only collectin known to be membrane bound, while CL-L1 (collectin liver-1) is the only known cytoplasmic collectin (1). Human CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that contains an N-terminal 39 aa cytoplasmic domain, a 17 aa transmembrane segment, and a 686 aa C-terminal extracellular region (6). The short cytoplasmic domain contains an internalization motif (Y-K-R-F) while the extracellular region is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure and a C-type lectin/ carbohydrate recognition domain (CRD). Notably, this CRD recognizes galactose (and fucose) within the context of asialo-orosomucoids associated with the Lewisx epitope (7, 8). CL-P1 has a 300 kDa trimeric form due to its collagen-like and coiled-coil helical domains (1, 5). There is a 97 kDa, alternate splice form of CL-P1 (SRCL type II) that shows a 120 aa truncation at the C-terminus. This effectively removes the entire CRD found on full-length CL-P1 (6). Human CL-P1 is 93% aa identical to mouse CL-P1 over the entire extracellular region, and 87% aa identical within each species CRD (5, 9). Human CL-P1 is known to be expressed in vascular endothelial cells (5). CL-P1 may play a role in bacterial recognition or as a scavenger receptor for desialylated.
- van de Wetering, JK. et al. (2004) Eur. J. Biochem. 271:1229.
- Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
- Hoppe, H-J. and K. Reid (1994) Protein Sci. 3:1143.
- Hickling, T.P. et al. (2004) J. Leukoc. Biol. 75:27.
- Ohtani, K. et al. (2001) J. Biol. Chem. 276:44222.
- Nakamura,K. et al. (2001) Biochem. Biophys. Res. Commun. 280:1028.
- Coombs, P.J. et al. (2005) J. Biol. Chem. 280:22993.
- Yoshida, T. et al. (2003) J. Biochem. 133:271.
- Nakamura, K. et al. (2001) Biochim. Biophys. Acta 1522:53.
Citation for Recombinant Human CL-P1/COLEC12 Protein, CF
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1 Citation: Showing 1 - 1
Soluble Collectin-12 (CL-12) Is a Pattern Recognition Molecule Initiating Complement Activation via the Alternative Pathway.
Authors: Ma Y, Hein E, Munthe-Fog L, Skjoedt M, Bayarri-Olmos R, Romani L, Garred P
J Immunol, 2015;195(7):3365-73.
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