Recombinant Human CLEC-1 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human CLEC-1 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles. The ED50 for this effect is 1-5 µg/mL.
Mouse myeloma cell line, NS0-derived human CLEC-1 protein
Gln77-Asp280, with an N-terminal 10-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
24.8 kDa
35-36 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: CLEC-1

CLEC-1 (C-type lectin-like receptor-1) is a type 2 transmembrane protein that belongs to the Dectin-1 family of C-type lectins (1, 2). It consists of a 52 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 207 aa extracellular domain (ECD) (3). The ECD contains a carbohydrate recognition domain (CRD) that includes cysteine residues with conserved spacing (4). In other C-type lectins, these cysteines form intrachain disulfide bonds that stabilize the CRD protein fold (4). CLEC-1 is a nonclassical C-type lectin in that its CRD lacks consensus amino acids which mediate calcium dependent carbohydrate binding in other C-type lectins (2, 3). The cytoplasmic domain contains one tyrosine, multiple serine and threonine, and a cluster of aspartic acid residues. The CLEC-1, CLEC-2, Dectin-1, and LOX-1 genes are located within the NK gene complex that also encodes several receptors involved in regulating NK cell activation (3, 5). CLEC-1 is most highly expressed in activated dendritic cells and at lower levels in endothelial cells and monocytes (3, 5). Within the CRD, human CLEC-1 shares 65% aa sequence identity with mouse and rat CLEC-1 and 26% - 36% with the other human Dectin-1 family lectins CLEC-2, CLEC9A, CLEC12B, Dectin-1, LOX-1, and MICL.

  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Pyz, E. et al. (2006) Ann. Med. 38:242.
  3. Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
  4. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  5. Sobanov, Y. et al. (2001) Eur. J. Immunol. 31:3493.
Long Name
C-type Lectin-like Receptor-1
Entrez Gene IDs
51267 (Human); 243653 (Mouse)
Alternate Names
CLEC1; CLEC-1; CLEC1A; CLEC1C-type lectin domain family 1 member A; C-type lectin domain family 1, member A; C-type lectin-like receptor 1; C-type lectin-like receptor-1; MGC34328

Citations for Recombinant Human CLEC-1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CLEC-1 is a death sensor that limits antigen cross-presentation by dendritic cells and represents a target for cancer immunotherapy
    Authors: M Drouin, J Saenz, V Gauttier, B Evrard, G Teppaz, S Pengam, C Mary, A Desselle, V Thepenier, E Wilhelm, E Merieau, C Ligeron, I Girault, MD Lopez, C Fourgeux, D Sinha, I Baccelli, A Moreau, C Louvet, R Josien, J Poschmann, N Poirier, E Chiffoleau
    Science Advances, 2022;8(46):eabo7621.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Capture
  2. Cell-surface C-type lectin-like receptor CLEC-1 dampens dendritic cell activation and downstream Th17 responses
    Authors: MD Lopez Robl, A Pallier, V Huchet, L Le Texier, S Remy, C Braudeau, L Delbos, A Moreau, C Louvet, C Brosseau, PJ Royer, A Magnan, F Halary, R Josien, MC Cuturi, I Anegon, E Chiffoleau
    Blood Adv, 2017;1(9):557-568.
    Species: Human
    Sample Types:
    Applications: ELISA (Standard)


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Recombinant Human CLEC-1 Protein, CF
By Philip Gorman on 05/15/2020
Application: Depletion Reagent
Reason for Rating: Our request for a custom bottling (large amount) was fulfilled very quickly. When we had questions about the quality of the protein, R&amp;D was quick to share their own internal QC and explain their findings.