Recombinant Human CLEC-1 Protein, CF

R&D Systems | Catalog # 1704-CL

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human CLEC-1 Protein (1704-CL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q8NC01

Applications

Binding Activity
View all CLEC-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CLEC-1 protein
Gln77-Asp280, with an N-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

24.8 kDa

SDS-PAGE

35-36 kDa, reducing conditions

Activity

Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles.
The ED50 for this effect is 1-5 µg/mL.

Reviewed Applications

Read 1 review rated 4 using 1704-CL in the following applications:

Formulation, Preparation, and Storage

1704-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLEC-1

CLEC-1 (C-type lectin-like receptor-1) is a type 2 transmembrane protein that belongs to the Dectin-1 family of C-type lectins (1, 2). It consists of a 52 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 207 aa extracellular domain (ECD) (3). The ECD contains a carbohydrate recognition domain (CRD) that includes cysteine residues with conserved spacing (4). In other C-type lectins, these cysteines form intrachain disulfide bonds that stabilize the CRD protein fold (4). CLEC-1 is a nonclassical C-type lectin in that its CRD lacks consensus amino acids which mediate calcium dependent carbohydrate binding in other C-type lectins (2, 3). The cytoplasmic domain contains one tyrosine, multiple serine and threonine, and a cluster of aspartic acid residues. The CLEC-1, CLEC-2, Dectin-1, and LOX-1 genes are located within the NK gene complex that also encodes several receptors involved in regulating NK cell activation (3, 5). CLEC-1 is most highly expressed in activated dendritic cells and at lower levels in endothelial cells and monocytes (3, 5). Within the CRD, human CLEC-1 shares 65% aa sequence identity with mouse and rat CLEC-1 and 26% - 36% with the other human Dectin-1 family lectins CLEC-2, CLEC9A, CLEC12B, Dectin-1, LOX-1, and MICL.

References

  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Pyz, E. et al. (2006) Ann. Med. 38:242.
  3. Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
  4. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  5. Sobanov, Y. et al. (2001) Eur. J. Immunol. 31:3493.

Long Name

C-type Lectin-like Receptor-1

Alternate Names

CLEC1, CLEC1A

Entrez Gene IDs

51267 (Human); 243653 (Mouse)

Gene Symbol

CLEC1A

UniProt

Q8NC01

Additional CLEC-1 Products

Product Documents for Recombinant Human CLEC-1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human CLEC-1 Protein, CF

For research use only

Citations for Recombinant Human CLEC-1 Protein, CF

Customer Reviews for Recombinant Human CLEC-1 Protein, CF (1)

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  • Recombinant Human CLEC-1 Protein, CF
    Name: Philip Gorman
    Application: Depletion Reagent
    Verified Customer | Posted 05/15/2020
    Recombinant Human CLEC-1 Protein, CF 1704-CL

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