Recombinant Human CMG-2 Protein, CF

Catalog # Availability Size / Price Qty
2940-CM-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human CMG-2 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind anthrax protective antigen (PA) in a functional ELISA. Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170.
Source
Mouse myeloma cell line, NS0-derived human CMG-2/ANTXR2 protein
Gln34-Asn317, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln34 predicted
Predicted Molecular Mass
31.4 kDa
SDS-PAGE
35-40 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2940-CM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CMG-2/ANTXR2

Capillary Morphogenesis Gene-2 (CMG-2) is a widely expressed anthrax toxin receptor (ATR) family protein (1 - 3). CMG-2 is a 55 kDa type I transmembrane (TM) protein that contains a 33 amino acid (aa) signal sequence, a 284 aa extracellular domain (ECD), a 24 aa TM segment, and a 147 aa cytoplasmic domain. There are three additional isoforms. Isoforms 4 shows a 12 aa insertion in the cytoplasmic region; isoform 2 shows a 103 aa deletion in the ECD; and isoform 3 is a truncated, 20 kDa, 289 aa soluble form. The main functional domain of CMG-2 is an extracellular integrin-like von Willebrand factor type A (VWA) domain with a metal ion dependent adhesion site (MIDAS). This domain adheres selectively to collagen type IV and laminin (1 - 5). CMG-2 isoform 2 is induced in HUVEC as they undergo capillary formation in collagen matrices in vitro (3). CMG-2 is mutated in juvenile hyaline fibromatosis and infantile systemic hyalinosis disorders, and several of these mutations result in loss of laminin binding (6). CMG-2 and the related protein ATR/TEM8 serve as receptors for the protective antigen (PA) of Bacillus Anthracis (1, 2). After binding the VWA domain, PA undergoes furin-type cleavage, forms a heptameric receptor/PA pre-pore and binds LF or EF toxin subunits (5, 7, 8). Transport to low pH endosomes, which requires CMG-2 ubiquitination and interaction with the LDL receptor related protein LRP6 (9, 10), allows PA pore formation and release of toxin to the cytoplasm (10, 11). Soluble CMG-2 VWA domain acts as a dummy receptor that can protect cultured cells from anthrax intoxication (2). Within the extracellular region, human CMG-2 shares 84%, 81%,89% and 93% amino acid sequence homology with mouse, rat, bovine, and canine CMG-2, respectively. CMG-2 VWA domain also shares 60% aa identity with ATR/TEM8.

References
  1. Scobie, H.M. and J.A.T. Young (2005) Curr. Opin. Microbiol. 8:106.
  2. Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170.
  3. Bell, S.E. et al. (2001) J. Cell Sci. 114:2755.
  4. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6367.
  5. Santelli, E. et al. (2004) Nature 430:905.
  6. Dowling, O. et al. (2003) Am. J. Hum. Genet. 73:957.
  7. Wigelsworth, D.J. et al. (2004) J. Biol. Chem. 279:23349.
  8. Go, M.Y. et al. (2006) J. Mol. Biol. 360:145.
  9. Abrami, L. et al. (2006) J. Cell Biol. 172:309.
  10. Wei, W. et al. (2006) Cell 124:1141.
  11. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13147.
Long Name
Capillary Morphogenesis Protein 2
Entrez Gene IDs
118429 (Human); 71914 (Mouse)
Alternate Names
anthrax toxin receptor 2; ANTXR2; Capillary morphogenesis gene 2 protein; capillary morphogenesis protein 2; cI-35; CMG2; CMG-2; CMG2MGC111533; CMG-2MGC45856; FLJ31074; ISH; JHF; JHS

Citation for Recombinant Human CMG-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Bacillus anthracis lethal toxin reduces human alveolar epithelial barrier function.
    Authors: Langer, Marybeth, Duggan, Elizabet, Booth, John Lel, Patel, Vineet I, Zander, Ryan A, Silasi-Mansat, Robert, Ramani, Vijay, Veres, Tibor Zo, Prenzler, Frauke, Sewald, Katherin, Williams, Daniel M, Coggeshall, Kenneth, Awasthi, Shanjana, Lupu, Florea, Burian, Dennis, Ballard, Jimmy Da, Braun, Armin, Metcalf, Jordan P
    Infect Immun, 2012;80(12):4374-87.
    Species: N/A
    Sample Types: N/A
    Applications: Western Blot

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