Recombinant Human Collectrin Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Supplemental Products

Recombinant Human Collectrin Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit proliferation of mIMCD‑3 mouse epithelial cells. The ED50 for this effect is 1.0-4.0 μg/mL.
Chinese Hamster Ovary cell line, CHO-derived human Collectrin protein
Human Collectrin
Accession # Q9HBJ8
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
41 kDa (monomer)
54-64 kDa, reducing conditions

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Collectrin

Collectrin, also known as TMEM27 and NX‑17, is a 35‑45 kDa transmembrane protein that shows structural similarity to the C‑terminal region of Angiotensin Converting Enzyme 2 (ACE‑2) but lacks peptidase activity (1, 2). Mature human Collectrin consists of a 127 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 60 aa cytoplasmic domain (3). Within the ECD, human Collectrin shares 87% and 89% aa sequence identity with mouse and rat Collectrin, respectively. Collectrin is expressed as a disulfide‑linked homodimer of variably glycosylated subunits (4‑6). It is highly expressed in the proximal convoluted tubules of renal collecting ducts and inhibits the proliferation of tubule epithelial cells (3, 6‑8). It associates with several amino acid transporters on the luminal surface of these cells, where it is required for the reabsorption of multiple amino acids (7‑9). Collectrin is also expressed in pancreatic islet beta cells and enhances glucose‑stimulated insulin secretion as well as insulin resistance (4, 5, 9, 10). In both the kidney and pancreas, cytoplasmic Collectrin interacts with multiple components of the SNARE complex and other vesicle trafficking proteins (5, 6). A 25 kDa glycosylated fragment of the ECD can be shed from both renal tubule cells and pancreatic beta cells (4, 10). In beta cells, shedding is mediated by BACE2, and the portion left in the membrane can be subsequently cleaved by gamma‑Secretase, releasing the intracellular domain into the cytosol (11). Inhibition of Collectrin shedding from beta cells results in increased beta cell mass and insulin secretion (11).

  1. Lambert, D.W. et al. (2010) Cell. Mol. Life Sci. 67:89.
  2. Zhang, Y. and J. Wada (2007) Biochem. Biophys. Res. Commun. 363:1.
  3. Zhang, H. et al. (2001) J. Biol. Chem. 276:17132.
  4. Akpinar, P. et al. (2005) Cell Metab. 2:385.
  5. Fukui, K. et al. (2005) Cell Metab. 2:373.
  6. Zhang, Y. et al. (2007) PLoS ONE 5:e414.
  7. Danilczyk, U. et al. (2006) Nature 444:1088.
  8. Malakauskas, S.M. et al. (2007) Am. J. Physiol. Renal Physiol. 292:F533.
  9. Malakauskas, S.M. et al. (2009) Mol. Endocrinol. 23:881.
  10. Altirriba, J. et al. (2010) Diabetologia 53:1406.
  11. Esterhazy, D. et al. (2011) Cell Metab. 14:365.
Entrez Gene IDs
57393 (Human); 57394 (Mouse); 57395 (Rat)
Alternate Names
Collectrin; kidney-specific membrane protein; NX17; NX-17; TMEM27; transmembrane protein 27NX170610008J07Rik


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human Collectrin Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Collectrin Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Human Collectrin Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review