Recombinant Human CTGF/CCN2 Protein, CF
Recombinant Human CTGF/CCN2 Protein, CF Summary
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Gln27-Ala349
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Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9190-CC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Immobilized Recombinant Human CTGF (Catalog # 9190-CC) supports the adhesion of Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 1.5-7.5 μg/mL.
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Background: CTGF/CCN2
Connective Tissue Growth Factor (CTGF), also known as CCN2, is a 36-38 kDa member of the CCN (CYR61/CTGF/NOV) family of secreted matricellular proteins (1). Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain (2). Human CTGF shares 94% amino acid (aa) sequence identity with mouse and rat CTGF. Alternative splicing generates an additional isoform with a 27 aa deletion within the TSP-1 domain. CTGF promotes cell adhesion through interactions with a range of cell surface molecules including heparan sulfate proteoglycans (HSPG), LRPAP, and Integrins alpha M, alpha V beta 3, alpha 6 beta 1, alpha IIb beta 3 (3-7). It also binds to and regulates signaling through the Wnt receptors LRP6 and Frizzled-8 and the NGF receptors TrkA and NGF R (8, 9). In addition, CTGF binds directly to BMP-4, TGF-beta 1, TGF-beta 2, and VEGF 165 (10, 11). It blocks BMP-4 and VEGF induced responses but enhances TGF-beta induced responses (10-13). Within VEGF complexes, CTGF can be degraded by a variety of proteases, resulting in restoration of angiogenic activity (11). CTGF promotes fibroblast differentiation from mesenchymal stem cells and their production of type I Collagen and Tenascin C (5, 14). It promotes chondrocyte proliferation and cartilage matrix synthesis (15-17). CTGF is expressed in vascular mural and endothelial cells (EC) during development and promotes pericyte-EC association and angiogenesis (2, 17, 18, 19). It is expressed in the cerebral cortex and olfactory bulb and plays an important role in nervous system development (8, 12).
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- Wahab, N.A. et al. (2005) J. Am. Soc. Nephrol. 16:340.
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- Hall-Glenn, F. et al. (2012) PLoS One 7:e30562.
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Citations for Recombinant Human CTGF/CCN2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Vaccination against connective tissue growth factor attenuates the development of renal fibrosis
Authors: T Nakayama, T Azegami, K Hayashi, A Hishikawa, N Yoshimoto, R Nakamichi, E Sugita, H Itoh
Scientific Reports, 2022;12(1):10933.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot Control -
mTOR inhibition prevents angiotensin II-induced aortic rupture and pseudoaneurysm but promotes dissection in Apoe-deficient mice
Authors: C He, B Jiang, M Wang, P Ren, SI Murtada, AW Caulk, G Li, L Qin, R Assi, CJ Lovoulos, MA Schwartz, JD Humphrey, G Tellides
JCI Insight, 2022;7(3):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Cathepsin K Regulates Intraocular Pressure by Modulating Extracellular Matrix Remodeling and Actin-Bundling in the Trabecular Meshwork Outflow Pathway
Authors: A Soundarara, SA Ghag, SS Vuda, T Wang, PP Pattabiram
Cells, 2021;10(11):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Identification of drivers of breast cancer invasion by secretome analysis: insight into CTGF signaling
Authors: JW Hellinger, F Schömel, JV Buse, C Lenz, G Bauerschmi, G Emons, C Gründker
Sci Rep, 2020;10(1):17889.
Species: Human
Sample Types: Organoid
Applications: Bioassay -
Complement C4A Regulates Autoreactive B Cells in Murine Lupus
Authors: L Simoni, J Presumey, CE van der Po, C Castrillon, SE Chang, PJ Utz, MC Carroll
Cell Rep, 2020;33(5):108330.
Species: Human
Sample Types: Protein
Applications: ELISA Standard -
IGF-1R deficiency in human keratinocytes disrupts epidermal homeostasis and stem cell maintenance
Authors: T Muraguchi, D Nanba, EK Nishimura, T Tashiro
J. Dermatol. Sci., 2019;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Identification of a peptide recognizing cerebrovascular changes in mouse models of Alzheimer's disease
Authors: AP Mann, P Scodeller, S Hussain, GB Braun, T Mölder, K Toome, R Ambasudhan, T Teesalu, SA Lipton, E Ruoslahti
Nat Commun, 2017;8(1):1403.
Applications: Bioassay
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