Recombinant Human CTGF/CCN2 Protein, CF

(1 Review)
Datasheet / CoA / SDS
Product Details
Supplemental Products
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Ball, D.K. et al. (2003) Reproduction 125:271. The ED50 for this effect is1.5-7.5 μg/mL. Also measured by its ability to induce C2C12 mouse myoblast cells proliferation.
  • Source
    Mouse myeloma cell line, NS0-derived human CTGF/CCN2 protein
  • Accession #
  • N-terminal Sequence
    No results obtained. Gln27 inferred from enzymatic pyroglutamate treatment revealing Asn28
  • Predicted Molecular Mass
    35 kDa
    35-45 kDa, reducing conditions
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Image
Immobilized Recombinant Human CTGF (Catalog # 9190-CC)supports the adhesion of Balb/3T3 mouse embryonic fibroblast cells. The ED50for this effect is
1.5-7.5 μg/mL.
Background: CTGF/CCN2
Connective Tissue Growth Factor (CTGF), also known as CCN2, is a 36-38 kDa member of the CCN (CYR61/CTGF/NOV) family of secreted matricellular proteins (1). Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain (2). Human CTGF shares 94% amino acid (aa) sequence identity with mouse and rat CTGF. Alternative splicing generates an additional isoform with a 27 aa deletion within the TSP-1 domain. CTGF promotes cell adhesion through interactions with a range of cell surface molecules including heparan sulfate proteoglycans (HSPG), LRPAP, and Integrins  alpha M, alpha V beta 3, alpha 6 beta 1, alpha IIb beta 3 (3-7). It also binds to and regulates signaling through the Wnt receptors LRP6 and Frizzled-8 and the NGF receptors TrkA and NGF R (8, 9). In addition, CTGF binds directly to BMP-4, TGF-beta 1, TGF-beta 2, and VEGF 165 (10, 11). It blocks BMP-4 and VEGF induced responses but enhances TGF-beta induced responses (10-13). Within VEGF complexes, CTGF can be degraded by a variety of proteases, resulting in restoration of angiogenic activity (11). CTGF promotes fibroblast differentiation from mesenchymal stem cells and their production of type I Collagen and Tenascin C (5, 14). It promotes chondrocyte proliferation and cartilage matrix synthesis (15-17). CTGF is expressed in vascular mural and endothelial cells (EC) during development and promotes pericyte-EC association and angiogenesis (2, 17, 18, 19). It is expressed in the cerebral cortex and olfactory bulb and plays an important role in nervous system development (8, 12).
  • References:
    1. Hall-Glenn, F. and K.M. Lyons (2011) Cell. Mol. Life Sci. 68:3209.
    2. Bradham, D.M. et al. (1991) J. Cell Biol. 114:1285.
    3. Gao, R. and D.R. Brigstock (2004) J. Biol. Chem. 279:8848.
    4. Schober, J.M. et al. (2002) Blood 99:4457.
    5. Heng, E.C.K. et al. (2006) J. Cell Biochem. 98:409.
    6. Jedsadayanmata, A. et al. (1999) J. Biol. Chem. 274:24321.
    7. Gao, R. et al. (2003) Hepatol. Res. 27:214.
    8. Mercurio, S. et al. (2004) Development 131:2137.
    9. Wahab, N.A. et al. (2005) J. Am. Soc. Nephrol. 16:340.
    10. Abreu, J.G. et al. (2002) Nat. Cell Biol. 4:599.
    11. Hashimoto, G. et al. (2002) J. Biol. Chem. 277:36288.
    12. Khodosevich, K. et al. (2013) Neuron 79:1136.
    13. Shi-Wen, X. et al. (2006) J. Biol. Chem. 281:10715.
    14. Lee, C.H. et al. (2010) J. Clin. Invest. 120:3340.
    15. Canalis, E. et al. (2010) Endocrinology 151:3490.
    16. Nakanishi, T. et al. (2000) Endocrinology 141:264.
    17. Ivkovic, S. et al. (2003) Development 130:2779.
    18. Hall-Glenn, F. et al. (2012) PLoS One 7:e30562.
    19. Shimo, T. et al. (1999) J. Biochem. 126:137.
  • Long Name:
    Connective Tissue Growth Factor
  • Entrez Gene IDs:
    1490 (Human); 14219 (Mouse); 64032 (Rat)
  • Alternate Names:
    CCN2; CCN2IGFBP-8; connective tissue growth factor; CTGF; CTGRP; Fisp12; HCS24; Hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP-8; IGFBP8CCN family member 2; Insulin-like growth factor-binding protein 8; MGC102839; NOV2
Related Research Areas


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 1 review)

Have you used Recombinant Human CTGF/CCN2 Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Recombinant Human CTGF/CCN2 Protein, CF.

We have 1 review tested in 1 application: Cell proliferation.

Filter by Ratings
All Ratings
Filter by Applications
Cell proliferation
All Applications
Filter by Species
All Species

Images Ratings Applications Species Reviewed By Date Details
Cell proliferation Recombinant Human CTGF/CCN2 Protein, CF 9190-CC Array 9190-CC
 Cell proliferation Anonymous 04/28/2018
Image Details
Cell proliferation Recombinant Human CTGF/CCN2 Protein, CF 9190-CC
Cell proliferation: Recombinant Human CTGF/CCN2 Protein, CF [9190-CC].


Application Cell proliferation

Please scroll the above reviews for more

Order Details

Contact Us to Order


Customer Information
You May Also Like

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

R&D Systems Guarantee