Recombinant Human CUL2/NEDD8/RBX1 Complex Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.|
Background: CUL2/NEDD8/RBX1 Complex
Cullin-2 is a core component of multiple cullin-RING based "ECS"
(Elongin B/C-CUL2-SOCS-box protein) E3 Ubiquitin ligase complexes that
mediate the ubiquitination of proteins involved in a variety of cellular
processes. In the ECS complex, Cullin-2 serves as a scaffold that
organizes the ELOB/C-associated recognition subunits with the RBX1
subunit and contributes to the catalysis through positioning of the
substrate and an E2 ubiquitin-conjugating enzyme. In vivo, the E3
ubiquitin ligase activity of the ECS complex is dependent on neddylation
of the cullin subunit, though neddylation may be dispensable for some
in vitro reactions. This complex consists of an N-terminal 10-His tagged
Cullin-2 (UniProt Q13617) and untagged RBX1 (P62877). CUL2 is neddylated in this complex.
- Baek K., et al. (2020) Nature 578: 461
- Duda D.M., et al. (2008) Cell 134: 995
- Duda D.M., et al. (2012) Mol. Cell 47: 371
- Goldenberg S.J., et al. (2004) Cell 119: 517
- Zheng N., et al. (2002) Nature 416: 703
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