Recombinant Human CUL4A/NEDD8/RBX1/DDB1/CRBN Protein, CF
Recombinant Human CUL4A/NEDD8/RBX1/DDB1/CRBN Protein, CF Summary
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Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E3-651
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: CUL4A/NEDD8/RBX1/DDB1/CRBN Complex
Cullin-4A is a core component of multiple cullin-RING type ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, DNA repair and other processes. In the DCX complex (DDB1-Cul4-x-box), Cullin-4A serves as a scaffold that organizes the DDB1-X-box recognition subunits with the Rbx1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme. In vivo, the E3 ubiquitin ligase activity of the SCF complex is dependent on the neddylation of the cullin subunit, though neddylation may be dispensable in some in vitro reactions. This complex consists of an N-terminal 10-His tagged, neddylated (UniProt Q15843) Cullin-4A (UniProt Q13619), untagged RBX1 (UniProt P62877), untagged DDB1 (UniProt Q16531) and untagged CRBN (UniProt Q96SW2).
- Fischer E.S., et al. (2014) Nature 512: 49
- He Y.J., et al. (2006) Genes Dev. 20: 2949
- Ito T., et al. (2010) Science 327: 1345
- Wang H., et al. (2006) Mol. Cell 22: 383
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