Recombinant Human Desmoglein-4 Fc Chimera Protein, CF

R&D Systems | Catalog # 9230-DM

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems HEK293-derived Recombinant Human Desmoglein-4 Fc Chimera Protein (9230-DM)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q86SJ6

Structure / Form

Disulfide-liked homodimer

Applications

Bioactivity
View all Desmoglein-4 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Desmoglein-4 protein
Human Desmoglein-4
(Glu24-Ala631)
Accession # Q86SJ6		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu24

Predicted Molecular Mass

94 kDa

SDS-PAGE

95-115 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.
The ED50 for this effect is 0.4-2.4 μg/mL.

Scientific Data Images for Recombinant Human Desmoglein-4 Fc Chimera Protein, CF

Recombinant Human Desmoglein-4 Fc Chimera Protein Bioactivity

Recombinant Human Desmoglein-4 Fc Chimera Protein Bioactivity

Immobilized Recombinant Human Desmoglein‑4 Fc Chimera (Catalog # 9230-DM) supports the adhesion of A431 human epithelial carcinoma cells. The ED50 for this effect is 0.4-2.4 μg/mL.

Formulation, Preparation, and Storage

9230-DM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Desmoglein-4

Desmoglein-4 is an approximately 140 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules. Human Desmoglein-4 is synthesized with a 26 amino acid (aa) propeptide followed by a 582 aa extracellular domain (ECD) with four tandem cadherin-like domains, a 21 aa transmembrane segment, and a 388 aa cytoplasmic domain (1, 2). Within the ECD, human Desmoglein-4 shares 82% aa sequence identity with mouse and rat Desmoglein-4. Alternative splicing generates additional isoform with a substitution in the cytoplasmic domain. Desmoglein-4 is the principal cadherin of desmosomes which form adhesive contacts between epithelial cells (2, 3). It is expressed in hair follicles and differentiated keratinocytes in suprabasal layers of the skin and plays a role in keratinocyte differentiation and adhesion (2, 3). Mutations in human Desmoglein-4 are associated with the development of monilethrix autosomal recessive hypotrichosis (LAH) (2, 4-8). Autoantibodies directed against Desmoglein-3 in the blistering skin disorder pemphigus vulgaris can cross-react with Desmoglein-4 (2, 9, 10).

References

  1. Whittock, N.V. and C. Bower (2003) J. Invest. Dermatol. 120:523.
  2. Kljuic, A. et al. (2003) Cell 113:249.
  3. Bazzi, H. et al. (2006) Differentiation 74:129.
  4. Bazzi, H. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:222.
  5. Schaffer, J.V. et al. (2006) J. Invest. Dermatol. 126:1286.
  6. Shimomura, Y. et al. (2006) J. Invest. Dermatol. 126:1281.
  7. Zlotogorski, A. et al. (2006) J. Invest. Dermatol. 126:1292.
  8. Kato, M. et al. (2015) J. Invest. Dermatol. 135:1253.
  9. Nagasaka, T. et al. (2004) J. Clin. Invest. 114:1484.
  10. Calkins, C.C. et al. (2006) J. Biol. Chem. 281:7623.

Alternate Names

Cad13, CDGF13, CDHF13, Desmoglein4, DSG4

Entrez Gene IDs

147409 (Human); 16769 (Mouse); 291754 (Rat)

Gene Symbol

DSG4

UniProt

Q86SJ6 (Human)

Additional Desmoglein-4 Products

Product Documents for Recombinant Human Desmoglein-4 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Desmoglein-4 Fc Chimera Protein, CF

For research use only

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

Customer Reviews for Recombinant Human Desmoglein-4 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Desmoglein-4 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Human Desmoglein-4 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes