Recombinant Human DGCR2 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10161-DG-050
Recombinant Human DGCR2 His-tag Protein, CF SDS-PAGE
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Recombinant Human DGCR2 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human DGCR2, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth. 
Source
Chinese Hamster Ovary cell line, CHO-derived human DGCR2 protein
Glu22-Ala349, with an C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu22
Predicted Molecular Mass
38 kDa
SDS-PAGE
40-60 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10161-DG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human DGCR2 His-tag (Catalog # 10161-DG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-60 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DGCR2

DiGeorge Syndrome Critical Region 2 (DGCR2), also known as IDD, DGS-C, LAN, and SEZ-12, is an adhesion receptor protein located on the long arm of chromosome 22 (1). The DGCR2 gene encodes an integral membrane protein, consisting of an extracellular domain, a single transmembrane region and a cytoplasmic tail (1). The mature extracellular domain (ECD) of DGCR2 contains both a C-type lectin domain and a cysteine-rich region similar to that of the low density lipoprotein receptor (LDLR) (1, 3). The mature ECD of human DGCR2 shares 93% and 92% amino acid sequence identity with mouse and rat, respectively. DGCR2 is expressed during neurodevelopment in human brain tissues (3). Deletion of the 22q11.2 region results in an extremely variable disorder called 22q11.2 deletion syndrome, with a phenotype ranging from very mild symptoms to severe intellectual disability, facial dysmorphism, heart defects, and urogenital abnormalities (4). Recent studies suggest that DGCR2 regulates critical steps of early cortico-genesis possibly through a Reelin-dependent mechanism. Deletion of DGCR2 has a pathogenic impact on cortical formation by reducing protein expression level, and it plays a critical role in vulnerability to schizophrenia (5). Furthermore, expression of DGCR2 together with USP18 gene may serve as a progonostic marker for muscle invasive bladder cancer survival in patients (5).

References
  1. Augusseau, S. et al. (1986) Hum. Genet. 74:206.
  2. Mugikura, S. et al. (2016) Biochem Biophys Rep. 5:120.
  3. Kajiwara, K. et al. (1996) Biochem. Biophyis. Res. Commun. 222:144.
  4. Vaisvilas, M. et al. (2018) Balkan J Med Genet. 21:87.
  5. Molinard-Chenu A. and Dayer A. (2017) Biol. Psych. 83:692.
Long Name
DiGeorge Syndrome Critical Region Gene 2
Entrez Gene IDs
9993 (Human); 13356 (Mouse); 360742 (Rat)
Alternate Names
DGCR2; DGS-C; DiGeorge syndrome critical region gene 2; IDDDKFZp686I1730; integral membrane protein deleted in DiGeorge syndrome; integral membrane protein DGCR2/IDD; KIAA0163; KIAA0163DiGeorge syndrome critical region protein 2; LAN; SEZ-12

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