Recombinant Human Dopamine beta-Hydroxylase Protein, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Reviews (1)

Recombinant Human Dopamine beta-Hydroxylase Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to convert tyramine to octopamine. The specific activity is >7,000 pmol/min/μg, as measured under the described conditions.
Chinese Hamster Ovary cell line, CHO-derived human Dopamine beta-Hydroxylase protein
Met1-Gly617, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Leu37 & Ser40
Predicted Molecular Mass
66 kDa
65-85 kDa, reducing conditions

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

  • Assay Buffer: 0.1 M NaOAc, 0.5 µM CuCl2, pH 5.0
  • Recombinant Human Dopamine beta ‑Hydroxylase (rhDBH) (Catalog # 7376-AO)
  • Substrate Component 1: Tyramine (Sigma, Catalog # T2879), 100 mM stock in deionized water
  • Substrate Component 2: N,N-Dimethyl-p-phenylenediamine (DMPD) (Sigma, Catalog # D4139), 500 mM stock in deionized water
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhDBH to 1 ng/µL in Assay Buffer.
  2. Dilute both substrate components to 40 mM respectively in Assay Buffer.
  3. Combine equivalent volumes of substrate components for substrate mixture.
  4. Load 50 µL of the diluted rhDBH into a clear plate, and start the reaction by adding 50 µL of the Substrate mixture to wells. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL Substrate Mixture without any rhDBH.
  5. Read in kinetic mode for 5 minutes at an absorbance of 515 nm.
  6. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/mol
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

     *Adjusted for Substrate Blank 
     **Using the extinction coefficient 5200 M-1cm-1 
     ***Using the path correction 0.32 cm
     Note: the output of many spectrophotometers is in mOD Per Well:
  • rhDBH: 0.050 μg
  • Tyramine: 10 mM
  • DMPD: 10 mM
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Dopamine beta-Hydroxylase

Dopamine beta -Hydroxylase (DBH), also known as dopamine beta -monooxygenase, belongs to the copper type II, ascorbate-dependent monooxygenase family. DBH is found within the neurosecretory vesicles of adrenal medullae and the large dense‑cored synaptic vesicles of the sympathetic nervous system as both membrane‑associated and soluble forms (1, 2). It catalyzes the conversion of dopamine to noradrenaline in sympathetic neurons, making it an important enzyme for catecholamine biosynthesis (3, 4). Mutations in the DBH gene that result in low DBH activity are a cause of noradrenaline deficiency (5). This recombinant human DBH is the secreted, soluble form.

  1. Dhawan, S. et al. (1987) J. Biol. Chem. 262:1869.
  2. Saxena, A. and P.J. Fleming (1983) J. Biol. Chem. 258:4147.
  3. Levin, E.Y. et al. (1960) J. Biol. Chem. 235:2080.
  4. Friedman, S. and S. Kaufman (1965) J. Biol. Chem. 240:4763.
  5. Kim, C.H. et al. (2002) Am. J. Med. Genet. 108:140.
Entrez Gene IDs
1621 (Human); 13166 (Mouse); 25699 (Rat)
Alternate Names
DBH; DBM; dopamine beta-hydroxylase (dopamine beta-monooxygenase); Dopamine betaHydroxylase; Dopamine beta-Hydroxylase; Dopamine beta-monooxygenase; DOPBHY; EC


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human Dopamine beta-Hydroxylase Protein, CF

Average Rating: 4 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Recombinant Human Dopamine beta-Hydroxylase Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Recombinant Human Dopamine beta-Hydroxylase Protein, CF
By Anonymous on 01/24/2017
Application: Enzymatic activity in vitro