Recombinant Human Dystroglycan Protein, CF

  
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells.

    The ED50 for this effect is 1.5-6.0 μg/mL.

    Optimal dilutions should be determined by each laboratory for each application.

  • Source
    Mouse myeloma cell line, NS0-derived Met1-Val749, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Gln28 predicted ( alpha subunit): No results obtained, sequencing might be blocked & Ser654 ( beta subunit)
  • Structure / Form
    Noncovalently-linked heterodimer
  • Predicted Molecular Mass
    67.8 kDa ( alpha subunit) & 11.3 kDa ( beta subunit)
  • SDS-PAGE
    65-90 kDa & 20 kDa, reducing conditions
6868-DG
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dystroglycan

Dystroglycan, also called DAG‑1 (dystrophin‑associated glycoprotein 1) or DG, is a heterodimeric adhesion molecule that links the extracellular matrix (ECM) to the cell cytoskeleton (1 ‑ 4). Human preproDAG‑1 is an 895 amino acid (aa) type I transmembrane protein that contains a 27 aa signal sequence and an 868 aa proform. Autocatalysis of the proform produces two fragments that remain noncovalently‑linked. The first fragment (or alpha ‑chain) is 626 aa in length (aa 28 ‑ 653) and contains a mucin‑like region, while the second fragment (or beta ‑chain) is a 42 ‑ 44 kDa, 242 aa N‑glycosylated protein with an extracellular (aa 654 ‑ 749), transmembrane, and cytoplasmic domain (5). Over aa 28 ‑ 749, human DAG‑1 shares 93% aa sequence identity with mouse DAG‑1. It is widely expressed but differentially O‑glycosylated on skeletal muscle and epithelia (which contain a 160 kDa alpha ‑chain) as compared to cardiac muscle, smooth muscle, fibroblasts, keratinocytes, lymphocytes, and hematopoietic stem cells (which contain a 100 ‑ 140 kDa alpha ‑chain) (1 ‑ 3, 6 ‑ 9). DAG‑1 binding of ECM molecules is influenced by its alpha ‑chain O‑glycosylation (2, 6 ‑ 10). In addition to skeletal muscle and neuromuscular junctions in which DAG‑1 binds several ECM molecules, DAG‑1 is important for neuronal migration (through neurexin interactions), keratinocyte attachment to the ECM (through laminin), and adhesion at the immunological synapse and in the hematopoietic stem cell niche (through agrin) (3, 6 ‑ 11). In muscle, the beta ‑chain cytoplasmic domain connects with the cytoskeleton via formation of the dystrophin‑glycoprotein complex with isoforms of dystrophin, sarcoglycan, syntrophin, and sarcospan (3). This complex is critical for skeletal muscle viability and regeneration (3, 4, 10, 11). MMP9 cleavage of the 44 kDa beta ‑chain creates a 30 kDa transmembrane form that causes dissociation of the heterodimer and a down‑regulation of ECM interactions (6, 12). Dystroglycanopathies, a group of congenital muscular dystrophies affecting the brain, eye and skeletal muscle, are caused by either abnormalities in glycosyltransferases, or their accessory proteins, or rare DAG‑1 polymorphisms. All result in DAG‑1 hypoglycosylation, especially of O‑mannosyl forms, and affect DAG‑1 binding to ECM proteins (2, 3, 10, 13, 14).

  • References:
    1. Ibraghimov-Bedkrovnaya, O. et al. (1993) Hum. Mol. Genet. 2:1651.
    2. Godfrey, C. et al. (2011) Curr. Opin. Genet. Dev. 21:278.
    3. Barresi, R. and Campbell, K.P. (2006) J. Cell Sci. 119:199.
    4. Durbeej, M. and K.P. Campbell (1999) J. Biol. Chem. 274:26609.
    5. Akhavan, A. et al. (2008) FASEB J. 22:612.
    6. Herzog, C. et al. (2004) J. Invest. Dermatol. 122:1372.
    7. Leonoudakis, D. et al. (2010) J. Cell Sci. 123:3683.
    8. Zhang, J. et al. (2006) FASEB J. 20:50.
    9. Mazzon, C. et al. (2011) Blood 118:2733.
    10. Michele, D.E. et al. (2002) Nature 418:417.
    11. Cohn, R.D. et al. (2002) Cell 110:639.
    12. Bozzi, M. et al. (2009) IUBMB Life 61:1143.
    13. Yoshida-Moriguchi, T. et al. (2010) Science 327:88.
    14. Hara, Y. et al. (2011) N. Eng. J. Med. 364:939.
  • Long Name:
    Dystrophin-associated Glycoprotein 1
  • Entrez Gene IDs:
    1605 (Human); 13138 (Mouse); 114489 (Rat)
  • Alternate Names:
    A3a; AGRNR; DAG1; Dag-1; DAG156DAG; dystroglycan 1 (dystrophin-associated glycoprotein 1); Dystroglycan; Dystrophin-associated glycoprotein 1
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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